Panax ginseng flexivirus 1
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346G7K3|A0A346G7K3_9VIRU Coat protein OS=Panax ginseng flexivirus 1 OX=2303411 PE=4 SV=1
MM1 pKa = 7.1 LQKK4 pKa = 10.84 DD5 pKa = 4.33 LLLGLLGAALVYY17 pKa = 10.34 LALVLVDD24 pKa = 3.75 NFKK27 pKa = 11.19 EE28 pKa = 4.38 SGCSIRR34 pKa = 11.84 LTGEE38 pKa = 3.8 SVSVINCQDD47 pKa = 3.38 LEE49 pKa = 4.29 AVAKK53 pKa = 10.13 LIRR56 pKa = 11.84 NSKK59 pKa = 9.35 PLWLSLGGRR68 pKa = 11.84 GDD70 pKa = 3.59 FEE72 pKa = 5.84 IIEE75 pKa = 4.27 EE76 pKa = 4.34 RR77 pKa = 11.84 CC78 pKa = 3.2
Molecular weight: 8.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.974
IPC2_protein 5.13
IPC_protein 4.851
Toseland 4.698
ProMoST 4.94
Dawson 4.774
Bjellqvist 4.927
Wikipedia 4.635
Rodwell 4.685
Grimsley 4.609
Solomon 4.774
Lehninger 4.724
Nozaki 4.889
DTASelect 5.003
Thurlkill 4.698
EMBOSS 4.647
Sillero 4.952
Patrickios 4.126
IPC_peptide 4.774
IPC2_peptide 4.94
IPC2.peptide.svr19 5.015
Protein with the highest isoelectric point:
>tr|A0A346G7K2|A0A346G7K2_9VIRU Movement protein TGBp3 OS=Panax ginseng flexivirus 1 OX=2303411 PE=3 SV=1
MM1 pKa = 7.71 SFQQPADD8 pKa = 2.97 WSKK11 pKa = 11.19 NLRR14 pKa = 11.84 PLLIGGGVALILFFFRR30 pKa = 11.84 QNNLPHH36 pKa = 6.57 TGDD39 pKa = 4.38 NIHH42 pKa = 6.49 SLPHH46 pKa = 5.99 GGQYY50 pKa = 10.37 QDD52 pKa = 2.8 GTKK55 pKa = 10.16 RR56 pKa = 11.84 INYY59 pKa = 7.75 CGPKK63 pKa = 10.08 KK64 pKa = 10.36 NFPGPGILSIGSSSTAFVILIVLVALIYY92 pKa = 10.53 ASEE95 pKa = 4.37 RR96 pKa = 11.84 FTARR100 pKa = 11.84 IVRR103 pKa = 11.84 RR104 pKa = 11.84 CPCVPGTCASRR115 pKa = 3.42
Molecular weight: 12.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.292
IPC2_protein 9.341
IPC_protein 9.575
Toseland 9.94
ProMoST 9.955
Dawson 10.189
Bjellqvist 9.955
Wikipedia 10.379
Rodwell 10.438
Grimsley 10.262
Solomon 10.248
Lehninger 10.218
Nozaki 10.116
DTASelect 9.897
Thurlkill 10.043
EMBOSS 10.365
Sillero 10.145
Patrickios 10.116
IPC_peptide 10.248
IPC2_peptide 9.268
IPC2.peptide.svr19 8.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2845
78
2139
569.0
64.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.975 ± 0.993
2.917 ± 0.465
5.8 ± 0.637
6.784 ± 0.894
6.432 ± 0.697
6.081 ± 0.669
2.601 ± 0.368
5.518 ± 0.564
6.995 ± 0.396
10.369 ± 0.791
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.25 ± 0.536
4.851 ± 0.25
3.409 ± 0.525
2.46 ± 0.309
5.835 ± 0.347
8.647 ± 0.35
3.339 ± 0.294
5.905 ± 0.688
1.09 ± 0.209
2.742 ± 0.523
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here