Tortoise microvirus 82
Average proteome isoelectric point is 7.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W6N1|A0A4P8W6N1_9VIRU Uncharacterized protein OS=Tortoise microvirus 82 OX=2583190 PE=4 SV=1
MM1 pKa = 6.99 QRR3 pKa = 11.84 VFNIDD8 pKa = 2.45 KK9 pKa = 7.42 WTLVEE14 pKa = 4.45 EE15 pKa = 4.77 GQSLGFLNDD24 pKa = 2.87 KK25 pKa = 9.47 PRR27 pKa = 11.84 NVRR30 pKa = 11.84 LEE32 pKa = 4.11 VNSPSEE38 pKa = 3.98 VEE40 pKa = 4.14 LWYY43 pKa = 10.51 ACQVSGEE50 pKa = 4.22 VHH52 pKa = 6.75 FLALVKK58 pKa = 10.74 GRR60 pKa = 11.84 DD61 pKa = 3.63 VVSFGSTGPFEE72 pKa = 4.1 ITVKK76 pKa = 10.83 NGFVMIATNDD86 pKa = 3.56 GDD88 pKa = 4.32 DD89 pKa = 3.74 VSMKK93 pKa = 9.04 PTAEE97 pKa = 4.04 KK98 pKa = 10.95 AFVKK102 pKa = 10.57 VMEE105 pKa = 4.23 RR106 pKa = 11.84 RR107 pKa = 11.84 ARR109 pKa = 11.84 DD110 pKa = 3.32 PHH112 pKa = 7.91 LEE114 pKa = 3.71 RR115 pKa = 11.84 MMQLMEE121 pKa = 3.9 VNMRR125 pKa = 11.84 RR126 pKa = 11.84 NQEE129 pKa = 3.66 QQAYY133 pKa = 8.28 EE134 pKa = 4.15 LEE136 pKa = 4.11 RR137 pKa = 11.84 LFARR141 pKa = 11.84 RR142 pKa = 11.84 VAALEE147 pKa = 3.98 AAASQGVGAGNVGDD161 pKa = 4.99 AGAEE165 pKa = 3.86 PAPQGTAGTAAKK177 pKa = 10.31 GDD179 pKa = 3.72 NSNGNDD185 pKa = 3.47 AGHH188 pKa = 6.76 DD189 pKa = 3.72 QSASAAGGAA198 pKa = 3.86
Molecular weight: 21.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.169
IPC2_protein 5.169
IPC_protein 5.054
Toseland 4.991
ProMoST 5.181
Dawson 5.029
Bjellqvist 5.169
Wikipedia 4.889
Rodwell 4.952
Grimsley 4.902
Solomon 5.029
Lehninger 4.978
Nozaki 5.143
DTASelect 5.283
Thurlkill 4.978
EMBOSS 4.927
Sillero 5.219
Patrickios 4.52
IPC_peptide 5.041
IPC2_peptide 5.219
IPC2.peptide.svr19 5.243
Protein with the highest isoelectric point:
>tr|A0A4P8W6U1|A0A4P8W6U1_9VIRU Uncharacterized protein OS=Tortoise microvirus 82 OX=2583190 PE=4 SV=1
MM1 pKa = 7.25 AVWVGVSKK9 pKa = 10.48 MKK11 pKa = 10.93 LIGEE15 pKa = 4.27 VLTRR19 pKa = 11.84 RR20 pKa = 11.84 VGTAVAAYY28 pKa = 10.07 LVGTLGIPADD38 pKa = 3.69 LAMKK42 pKa = 9.92 FVLAGGVLLGIGADD56 pKa = 3.62 VAIAAALARR65 pKa = 11.84 FRR67 pKa = 4.58
Molecular weight: 6.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.273
IPC2_protein 9.648
IPC_protein 10.204
Toseland 10.687
ProMoST 10.906
Dawson 10.774
Bjellqvist 10.423
Wikipedia 10.935
Rodwell 11.125
Grimsley 10.804
Solomon 10.877
Lehninger 10.847
Nozaki 10.643
DTASelect 10.423
Thurlkill 10.672
EMBOSS 11.067
Sillero 10.687
Patrickios 10.979
IPC_peptide 10.877
IPC2_peptide 9.019
IPC2.peptide.svr19 8.792
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1865
67
547
266.4
29.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.831 ± 1.022
0.858 ± 0.38
5.094 ± 0.457
5.201 ± 0.528
3.7 ± 0.255
8.847 ± 0.757
2.735 ± 0.629
4.236 ± 0.431
3.914 ± 0.608
6.81 ± 0.4
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.681 ± 0.43
4.236 ± 0.515
6.166 ± 0.737
4.933 ± 0.569
7.614 ± 0.976
5.094 ± 0.667
5.308 ± 0.79
7.399 ± 0.662
1.716 ± 0.318
2.627 ± 0.619
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here