Capybara microvirus Cap1_SP_148
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W7Y6|A0A4P8W7Y6_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_148 OX=2585392 PE=4 SV=1
MM1 pKa = 7.26 CLKK4 pKa = 10.35 YY5 pKa = 10.43 KK6 pKa = 10.56 YY7 pKa = 8.05 LTPVPPLPDD16 pKa = 2.82 ATFYY20 pKa = 11.65 EE21 pKa = 5.09 EE22 pKa = 4.06 VHH24 pKa = 6.12 TPNGYY29 pKa = 9.42 KK30 pKa = 10.24 VSEE33 pKa = 4.35 EE34 pKa = 3.81 PLYY37 pKa = 10.47 PPVSAFSIEE46 pKa = 4.01 NLLKK50 pKa = 10.73 SGIQIYY56 pKa = 9.09 PSPYY60 pKa = 8.69 LRR62 pKa = 11.84 PMSRR66 pKa = 11.84 FNVLSSAEE74 pKa = 4.05 RR75 pKa = 11.84 FLNTPEE81 pKa = 4.61 LVEE84 pKa = 5.57 ASQKK88 pKa = 8.39 WSEE91 pKa = 4.03 MTEE94 pKa = 4.06 EE95 pKa = 4.71 FLSQAQKK102 pKa = 10.6 IDD104 pKa = 3.56 TSNDD108 pKa = 3.23 LPDD111 pKa = 4.84 DD112 pKa = 3.88 KK113 pKa = 11.7
Molecular weight: 12.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.633
IPC2_protein 4.762
IPC_protein 4.609
Toseland 4.469
ProMoST 4.698
Dawson 4.533
Bjellqvist 4.685
Wikipedia 4.38
Rodwell 4.444
Grimsley 4.38
Solomon 4.533
Lehninger 4.482
Nozaki 4.647
DTASelect 4.749
Thurlkill 4.469
EMBOSS 4.406
Sillero 4.711
Patrickios 3.528
IPC_peptide 4.546
IPC2_peptide 4.711
IPC2.peptide.svr19 4.672
Protein with the highest isoelectric point:
>tr|A0A4P8W5G6|A0A4P8W5G6_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_148 OX=2585392 PE=4 SV=1
MM1 pKa = 7.39 SLLGGILGGASSLLGGAINAFTAYY25 pKa = 10.43 DD26 pKa = 3.68 LQNQQFAEE34 pKa = 4.18 QAKK37 pKa = 9.44 QRR39 pKa = 11.84 DD40 pKa = 3.57 WQEE43 pKa = 3.93 RR44 pKa = 11.84 MMDD47 pKa = 4.0 KK48 pKa = 10.79 EE49 pKa = 3.92 NAYY52 pKa = 8.33 NTDD55 pKa = 2.9 MWNKK59 pKa = 8.41 TSEE62 pKa = 4.13 FNEE65 pKa = 3.67 RR66 pKa = 11.84 MMRR69 pKa = 11.84 AQMEE73 pKa = 4.42 NASRR77 pKa = 11.84 MQTEE81 pKa = 3.98 QNVWNSEE88 pKa = 3.66 QAQYY92 pKa = 10.97 QRR94 pKa = 11.84 YY95 pKa = 8.43 KK96 pKa = 10.5 ATGLNPLGFGGNSSTSPAIQAGSASMSPQSSAAPPMPAQYY136 pKa = 11.65 ANMINPRR143 pKa = 11.84 WGDD146 pKa = 3.41 MLTNIAQGAEE156 pKa = 3.84 LGKK159 pKa = 10.84 VFGEE163 pKa = 4.08 TRR165 pKa = 11.84 KK166 pKa = 10.49 LKK168 pKa = 10.89 EE169 pKa = 3.6 EE170 pKa = 4.15 TKK172 pKa = 10.65 NIEE175 pKa = 3.57 VDD177 pKa = 3.34 RR178 pKa = 11.84 LKK180 pKa = 11.13 KK181 pKa = 10.54 LVEE184 pKa = 4.09 TQLNKK189 pKa = 10.74 AQIAKK194 pKa = 9.82 LAEE197 pKa = 4.15 EE198 pKa = 4.43 VVEE201 pKa = 4.17 TRR203 pKa = 11.84 QRR205 pKa = 11.84 ISQINLDD212 pKa = 3.94 NEE214 pKa = 4.01 AKK216 pKa = 10.51 EE217 pKa = 4.43 IINKK221 pKa = 9.74 YY222 pKa = 9.61 LDD224 pKa = 3.79 RR225 pKa = 11.84 NSALQLRR232 pKa = 11.84 HH233 pKa = 6.52 LEE235 pKa = 4.16 LEE237 pKa = 3.98 NLQTQKK243 pKa = 9.05 QTDD246 pKa = 3.97 KK247 pKa = 10.81 IKK249 pKa = 11.22 SEE251 pKa = 3.55 ISKK254 pKa = 10.36 IKK256 pKa = 9.86 TDD258 pKa = 3.14 ISLSEE263 pKa = 4.09 SEE265 pKa = 4.24 RR266 pKa = 11.84 KK267 pKa = 9.65 KK268 pKa = 10.73 VEE270 pKa = 3.94 QFVSQQSKK278 pKa = 10.45 LFPQEE283 pKa = 3.92 LQEE286 pKa = 4.63 KK287 pKa = 9.06 FLKK290 pKa = 10.86 NEE292 pKa = 4.04 LSSKK296 pKa = 10.07 QSQEE300 pKa = 3.78 FSNKK304 pKa = 9.54 LKK306 pKa = 10.13 TFDD309 pKa = 4.91 LEE311 pKa = 4.24 YY312 pKa = 10.97 EE313 pKa = 4.11 KK314 pKa = 11.14 LKK316 pKa = 11.38 NEE318 pKa = 4.57 LKK320 pKa = 10.97 LSDD323 pKa = 3.96 AQLLDD328 pKa = 3.87 LLRR331 pKa = 11.84 VEE333 pKa = 4.66 QNLKK337 pKa = 10.3 IPFFGNFKK345 pKa = 10.5 FYY347 pKa = 10.84 TPAKK351 pKa = 9.56 LKK353 pKa = 10.38 EE354 pKa = 4.05 NKK356 pKa = 9.52 LMKK359 pKa = 10.12 NQQSRR364 pKa = 11.84 YY365 pKa = 9.48 NIRR368 pKa = 11.84 SDD370 pKa = 3.02 PRR372 pKa = 11.84 RR373 pKa = 11.84 RR374 pKa = 11.84 DD375 pKa = 3.45 FMDD378 pKa = 4.4 FPSYY382 pKa = 11.28 VNPP385 pKa = 4.2
Molecular weight: 44.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.527
IPC2_protein 8.419
IPC_protein 8.331
Toseland 9.253
ProMoST 8.916
Dawson 9.472
Bjellqvist 9.092
Wikipedia 9.604
Rodwell 9.853
Grimsley 9.531
Solomon 9.516
Lehninger 9.487
Nozaki 9.194
DTASelect 9.092
Thurlkill 9.311
EMBOSS 9.663
Sillero 9.37
Patrickios 5.27
IPC_peptide 9.516
IPC2_peptide 7.439
IPC2.peptide.svr19 7.67
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1588
86
582
317.6
36.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.975 ± 0.699
1.071 ± 0.385
6.108 ± 0.744
5.668 ± 1.411
5.982 ± 0.795
4.156 ± 0.79
1.889 ± 0.684
4.03 ± 0.385
6.423 ± 1.369
9.824 ± 1.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.204 ± 0.529
6.234 ± 0.693
4.534 ± 0.962
5.227 ± 1.23
5.227 ± 0.825
7.997 ± 1.187
5.856 ± 0.721
5.353 ± 1.07
1.259 ± 0.206
5.982 ± 1.26
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here