Cellulophaga phage phi46:1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S0A0T6|S0A0T6_9CAUD Uncharacterized protein OS=Cellulophaga phage phi46:1 OX=1327974 GN=Phi46:1_gp22 PE=4 SV=1
MM1 pKa = 7.97KK2 pKa = 10.51DD3 pKa = 3.28FLEE6 pKa = 4.48NLAEE10 pKa = 4.24INGWGFDD17 pKa = 3.49YY18 pKa = 11.02GRR20 pKa = 11.84QDD22 pKa = 3.87FLNLFDD28 pKa = 4.1GTHH31 pKa = 6.92DD32 pKa = 3.79EE33 pKa = 5.08KK34 pKa = 11.24IHH36 pKa = 6.24IACDD40 pKa = 3.77PIKK43 pKa = 10.03IDD45 pKa = 3.64RR46 pKa = 11.84TYY48 pKa = 10.25TPAGTVEE55 pKa = 4.62SVSNTCSFLIGLSSDD70 pKa = 3.56YY71 pKa = 11.37DD72 pKa = 3.71EE73 pKa = 5.72EE74 pKa = 4.49NYY76 pKa = 10.34KK77 pKa = 10.38YY78 pKa = 10.63RR79 pKa = 11.84YY80 pKa = 10.03DD81 pKa = 4.0NYY83 pKa = 10.26IKK85 pKa = 10.6PLLDD89 pKa = 3.59EE90 pKa = 4.3KK91 pKa = 11.3LKK93 pKa = 10.7IIEE96 pKa = 4.3NGVRR100 pKa = 11.84CSGNMEE106 pKa = 4.07IVSLGSIEE114 pKa = 4.26VVNSNLFDD122 pKa = 4.6FNFDD126 pKa = 3.46GLLINCVIKK135 pKa = 10.42IYY137 pKa = 11.02

Molecular weight: 15.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0A0W0|S0A0W0_9CAUD Uncharacterized protein OS=Cellulophaga phage phi46:1 OX=1327974 GN=Phi46:1_gp47 PE=4 SV=1
MM1 pKa = 7.56LGLHH5 pKa = 6.26ARR7 pKa = 11.84GLRR10 pKa = 11.84LLARR14 pKa = 11.84CLLPRR19 pKa = 11.84GKK21 pKa = 10.02KK22 pKa = 9.2RR23 pKa = 11.84NRR25 pKa = 11.84IKK27 pKa = 10.17RR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.16RR31 pKa = 11.84KK32 pKa = 8.22RR33 pKa = 11.84TRR35 pKa = 11.84KK36 pKa = 7.8RR37 pKa = 11.84TKK39 pKa = 10.05EE40 pKa = 3.84GG41 pKa = 3.17

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

10734

41

849

198.8

22.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.922 ± 0.645

0.894 ± 0.145

5.636 ± 0.251

7.5 ± 0.401

5.031 ± 0.282

5.953 ± 0.328

1.593 ± 0.183

7.183 ± 0.225

9.419 ± 0.443

9.298 ± 0.332

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.003 ± 0.149

6.046 ± 0.328

2.767 ± 0.222

3.112 ± 0.186

4.146 ± 0.213

6.475 ± 0.264

5.702 ± 0.243

5.674 ± 0.237

0.876 ± 0.125

3.773 ± 0.303
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski