Pseudidiomarina atlantica
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2290 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A094J8Z5|A0A094J8Z5_9GAMM LysR family transcriptional regulator OS=Pseudidiomarina atlantica OX=1517416 GN=IDAT_05205 PE=3 SV=1
MM1 pKa = 7.21 VSNNKK6 pKa = 9.71 FIYY9 pKa = 10.19 GLTFALILLSGSAQAATYY27 pKa = 10.23 DD28 pKa = 3.63 FRR30 pKa = 11.84 KK31 pKa = 10.01 NLPPGCSQKK40 pKa = 10.96 GNSPAQCPNGLTLQWNDD57 pKa = 2.91 VLVFDD62 pKa = 6.1 KK63 pKa = 10.74 IAEE66 pKa = 4.27 VNVTGNVWLSNAQVFLGANTPLTINATGSISSDD99 pKa = 2.41 STMQMFGDD107 pKa = 4.6 LNAVGQISLGSDD119 pKa = 3.02 NQVQGNLNSQNQITLNNNTDD139 pKa = 3.33 VTGSLVAPQVSLVSSNTITGPISATQLTLGFNSQVTGNLNVTNNLSVGSSSSITGSINAGNLQSNSPVSLNGPIVVDD216 pKa = 3.54 NEE218 pKa = 3.93 FNLASGSTVNGSVSANRR235 pKa = 11.84 VTLAPSNSTLTGDD248 pKa = 3.28 VTANEE253 pKa = 3.97 NVIIGSGNTIDD264 pKa = 4.24 GNVAAGFIRR273 pKa = 11.84 LEE275 pKa = 4.25 ASNATITGNAIATGNIVIDD294 pKa = 3.41 WAGQIGGDD302 pKa = 3.34 ATAVNITNNAGNTDD316 pKa = 3.91 SVGGTAFCDD325 pKa = 3.7 TSDD328 pKa = 3.44 GAQPLSCQTGSGGGGATQCDD348 pKa = 4.14 ALNDD352 pKa = 3.87 LVDD355 pKa = 3.61 YY356 pKa = 10.85 GVIGDD361 pKa = 4.09 SGFTYY366 pKa = 10.81 GNNSEE371 pKa = 4.29 INGTEE376 pKa = 3.44 IDD378 pKa = 5.1 DD379 pKa = 4.58 PNNTNGNTPTPTGVVEE395 pKa = 4.11 EE396 pKa = 5.0 AVVQFPPLTPNVFPSFNGGNSFTNPANLGPGTYY429 pKa = 10.38 NEE431 pKa = 4.84 INLSGNNASASLTGGGTYY449 pKa = 10.3 QIDD452 pKa = 4.19 SINFGPQSQTLTLGPGDD469 pKa = 3.61 YY470 pKa = 10.26 FVRR473 pKa = 11.84 TISLNNDD480 pKa = 2.95 STIAIAPAGPVRR492 pKa = 11.84 IFIEE496 pKa = 4.51 DD497 pKa = 4.07 SITGGNNLFFNPTGAVQNLMINLYY521 pKa = 10.36 DD522 pKa = 3.82 GASFEE527 pKa = 4.46 IGNYY531 pKa = 8.27 NQGSTTYY538 pKa = 9.24 TFNGVLYY545 pKa = 10.64 APYY548 pKa = 9.43 TSNDD552 pKa = 2.55 ITFGNNSNLQGAVLSAGTVDD572 pKa = 3.09 IGNNTSINFTDD583 pKa = 3.78 AVQDD587 pKa = 3.66 AVRR590 pKa = 11.84 DD591 pKa = 3.78 AFGCDD596 pKa = 3.1 TEE598 pKa = 4.56 VTDD601 pKa = 3.15 IHH603 pKa = 7.0 HH604 pKa = 6.46 YY605 pKa = 10.21 RR606 pKa = 11.84 IQHH609 pKa = 5.81 PLSVVSCVAAPVQVVACLDD628 pKa = 3.47 ASCASRR634 pKa = 11.84 YY635 pKa = 8.26 TDD637 pKa = 3.15 ATEE640 pKa = 3.99 VALSSSATGSTFANGGVLGFTGGQGTLGLRR670 pKa = 11.84 YY671 pKa = 9.72 VDD673 pKa = 4.48 GGTTTVSLSNGAPAAQNPTEE693 pKa = 4.9 CYY695 pKa = 10.35 DD696 pKa = 3.65 SAGTATTNCEE706 pKa = 3.66 IEE708 pKa = 4.2 FRR710 pKa = 11.84 TAGLIFTANDD720 pKa = 3.76 GLSAIPPSFAGTDD733 pKa = 3.8 FDD735 pKa = 4.01 VLARR739 pKa = 11.84 AVEE742 pKa = 4.57 TNTQTGACEE751 pKa = 3.77 ARR753 pKa = 11.84 LTGAQVLEE761 pKa = 4.54 LATSCRR767 pKa = 11.84 NPLTCQAGQNFIANGANVPLNDD789 pKa = 3.8 AGATLSYY796 pKa = 11.31 ANVPVTFDD804 pKa = 3.58 ANGNAPLVANYY815 pKa = 9.68 TDD817 pKa = 3.55 VGALRR822 pKa = 11.84 LHH824 pKa = 6.09 GRR826 pKa = 11.84 VNLSEE831 pKa = 4.68 APNADD836 pKa = 3.35 EE837 pKa = 6.05 SVIDD841 pKa = 4.75 DD842 pKa = 4.09 PAITLTGTSVNDD854 pKa = 3.73 FVVKK858 pKa = 9.9 PHH860 pKa = 5.69 TLRR863 pKa = 11.84 LFAVDD868 pKa = 3.78 NANQAVSATTSGGSAFATAGDD889 pKa = 4.08 NFSLIITAEE898 pKa = 4.04 NANGDD903 pKa = 3.48 ATPNFGRR910 pKa = 11.84 EE911 pKa = 3.82 TPAAEE916 pKa = 4.06 AQASYY921 pKa = 11.11 VQTLYY926 pKa = 10.49 PSTATAPASDD936 pKa = 3.88 FVGTQGWQLDD946 pKa = 3.94 TNRR949 pKa = 11.84 AGSLRR954 pKa = 11.84 TDD956 pKa = 3.23 SARR959 pKa = 11.84 WLDD962 pKa = 3.4 VGTIEE967 pKa = 5.65 LAPGLNGNSYY977 pKa = 10.81 LGAGDD982 pKa = 3.63 VAAKK986 pKa = 10.36 QNATVGRR993 pKa = 11.84 FAPFRR998 pKa = 11.84 FALNTSAVANSCVAGDD1014 pKa = 3.95 FTYY1017 pKa = 10.2 MSEE1020 pKa = 4.04 PALTVSYY1027 pKa = 10.42 EE1028 pKa = 4.03 LYY1030 pKa = 10.94 AVDD1033 pKa = 3.38 SDD1035 pKa = 4.58 GNITEE1040 pKa = 5.03 NYY1042 pKa = 9.57 DD1043 pKa = 3.17 SSGYY1047 pKa = 10.59 SGTAEE1052 pKa = 3.94 VGFAAANLAPADD1064 pKa = 3.76 TATDD1068 pKa = 3.74 RR1069 pKa = 11.84 FADD1072 pKa = 3.84 RR1073 pKa = 11.84 LLNLAAQQAWTAGVMTFNAANAGFNRR1099 pKa = 11.84 HH1100 pKa = 6.21 PDD1102 pKa = 3.48 GNIDD1106 pKa = 3.81 GPFASLQLGLRR1117 pKa = 11.84 VLSEE1121 pKa = 3.4 IDD1123 pKa = 3.24 NRR1125 pKa = 11.84 NFAGSDD1131 pKa = 3.23 LTLAAVAGAAAPLNGTAQLRR1151 pKa = 11.84 YY1152 pKa = 9.96 GRR1154 pKa = 11.84 LVLEE1158 pKa = 4.12 NTYY1161 pKa = 10.78 GPEE1164 pKa = 3.99 NEE1166 pKa = 4.67 DD1167 pKa = 3.74 LSVPLYY1173 pKa = 10.99 AEE1175 pKa = 4.18 YY1176 pKa = 10.6 FDD1178 pKa = 3.91 GSRR1181 pKa = 11.84 FVTHH1185 pKa = 7.11 TDD1187 pKa = 3.6 DD1188 pKa = 3.83 SCSSALVAGLNVIADD1203 pKa = 4.36 PNNLTPVAAGSDD1215 pKa = 3.62 STLTDD1220 pKa = 3.17 GALPFDD1226 pKa = 4.15 TLRR1229 pKa = 11.84 WLAPGTGNTGEE1240 pKa = 4.72 FIYY1243 pKa = 10.41 EE1244 pKa = 3.69 YY1245 pKa = 10.49 DD1246 pKa = 3.41 APAWLEE1252 pKa = 3.93 FEE1254 pKa = 4.37 WRR1256 pKa = 11.84 DD1257 pKa = 3.43 EE1258 pKa = 4.15 AGAMYY1263 pKa = 10.56 LDD1265 pKa = 4.02 PRR1267 pKa = 11.84 AIGGFGQYY1275 pKa = 9.73 RR1276 pKa = 11.84 GNSRR1280 pKa = 11.84 VLFWKK1285 pKa = 10.07 EE1286 pKa = 3.54 VNN1288 pKa = 3.47
Molecular weight: 132.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.859
IPC_protein 3.897
Toseland 3.668
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.821
Rodwell 3.719
Grimsley 3.567
Solomon 3.884
Lehninger 3.846
Nozaki 3.986
DTASelect 4.253
Thurlkill 3.719
EMBOSS 3.834
Sillero 4.012
Patrickios 0.846
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.892
Protein with the highest isoelectric point:
>tr|A0A094IVG5|A0A094IVG5_9GAMM Uncharacterized protein OS=Pseudidiomarina atlantica OX=1517416 GN=IDAT_01505 PE=4 SV=1
MM1 pKa = 7.42 KK2 pKa = 10.05 RR3 pKa = 11.84 QKK5 pKa = 10.3 RR6 pKa = 11.84 DD7 pKa = 3.2 RR8 pKa = 11.84 LEE10 pKa = 3.91 RR11 pKa = 11.84 AHH13 pKa = 6.72 AQGFKK18 pKa = 10.64 AGVAGRR24 pKa = 11.84 SKK26 pKa = 10.44 EE27 pKa = 3.81 LCPYY31 pKa = 9.54 QNQEE35 pKa = 5.09 IRR37 pKa = 11.84 SQWLGGWRR45 pKa = 11.84 DD46 pKa = 3.4 AQEE49 pKa = 3.92 ARR51 pKa = 11.84 SVGLYY56 pKa = 9.82 RR57 pKa = 5.42
Molecular weight: 6.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.692
IPC_protein 10.57
Toseland 10.745
ProMoST 10.921
Dawson 10.833
Bjellqvist 10.57
Wikipedia 11.067
Rodwell 11.008
Grimsley 10.877
Solomon 10.979
Lehninger 10.95
Nozaki 10.73
DTASelect 10.555
Thurlkill 10.745
EMBOSS 11.155
Sillero 10.774
Patrickios 10.804
IPC_peptide 10.994
IPC2_peptide 9.604
IPC2.peptide.svr19 8.761
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2290
0
2290
760291
35
1613
332.0
36.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.48 ± 0.059
0.867 ± 0.019
5.605 ± 0.038
6.252 ± 0.047
3.935 ± 0.03
7.084 ± 0.05
2.28 ± 0.026
5.432 ± 0.037
3.82 ± 0.045
10.648 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.436 ± 0.023
3.455 ± 0.038
4.25 ± 0.03
5.307 ± 0.056
5.641 ± 0.041
5.537 ± 0.035
5.219 ± 0.032
7.34 ± 0.042
1.419 ± 0.023
2.993 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here