Natronincola ferrireducens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Natronincola

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2920 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G8YMF9|A0A1G8YMF9_9CLOT Putative competence-damage inducible protein OS=Natronincola ferrireducens OX=393762 GN=cinA PE=3 SV=1
MM1 pKa = 6.98NHH3 pKa = 6.44WKK5 pKa = 9.83PGQFAKK11 pKa = 10.55ILLALMLIVALLVLTGCGGGTGDD34 pKa = 3.34TTAPSEE40 pKa = 4.13EE41 pKa = 4.33SQEE44 pKa = 4.17EE45 pKa = 4.48VVDD48 pKa = 4.23RR49 pKa = 11.84DD50 pKa = 3.56DD51 pKa = 6.48DD52 pKa = 4.37EE53 pKa = 6.68DD54 pKa = 3.96VDD56 pKa = 4.09RR57 pKa = 11.84KK58 pKa = 10.8KK59 pKa = 11.24GFAHH63 pKa = 7.11FADD66 pKa = 3.99EE67 pKa = 4.47WVVPTIQPPLEE78 pKa = 4.02FVGNDD83 pKa = 3.37GFDD86 pKa = 3.61EE87 pKa = 4.84QILCSTGPTIVTISPEE103 pKa = 3.92GDD105 pKa = 3.09FLRR108 pKa = 11.84RR109 pKa = 11.84IVSSGKK115 pKa = 10.22GYY117 pKa = 10.66YY118 pKa = 9.78DD119 pKa = 3.91YY120 pKa = 11.14PVLSPDD126 pKa = 2.98GTRR129 pKa = 11.84FVVNADD135 pKa = 3.06YY136 pKa = 11.38HH137 pKa = 6.17EE138 pKa = 5.54FIEE141 pKa = 4.56PWEE144 pKa = 3.99MMLLGDD150 pKa = 4.43MDD152 pKa = 3.94TGEE155 pKa = 4.89LIEE158 pKa = 5.36LEE160 pKa = 4.22AAGDD164 pKa = 3.9YY165 pKa = 10.79YY166 pKa = 11.26YY167 pKa = 11.52YY168 pKa = 9.79PASWNHH174 pKa = 6.85DD175 pKa = 3.66GTKK178 pKa = 10.32VIYY181 pKa = 8.81TAYY184 pKa = 10.74DD185 pKa = 3.75EE186 pKa = 5.66DD187 pKa = 3.9WLANIYY193 pKa = 10.29YY194 pKa = 10.22VDD196 pKa = 3.87VDD198 pKa = 3.83TMEE201 pKa = 4.49SGIFLDD207 pKa = 5.0LAEE210 pKa = 4.8LPIDD214 pKa = 3.61RR215 pKa = 11.84EE216 pKa = 4.32VVEE219 pKa = 4.44TAKK222 pKa = 10.75LHH224 pKa = 5.46PNKK227 pKa = 10.5NILYY231 pKa = 9.39FIALPEE237 pKa = 4.29GEE239 pKa = 4.21EE240 pKa = 4.28DD241 pKa = 3.81YY242 pKa = 11.67GVFKK246 pKa = 11.19YY247 pKa = 10.91DD248 pKa = 3.29LDD250 pKa = 4.15EE251 pKa = 4.87GVLEE255 pKa = 4.32QVWTTEE261 pKa = 3.53EE262 pKa = 4.47EE263 pKa = 4.44YY264 pKa = 11.14VYY266 pKa = 10.48DD267 pKa = 3.56IAISPRR273 pKa = 11.84DD274 pKa = 3.72DD275 pKa = 4.07LLAISAGMGITEE287 pKa = 4.58EE288 pKa = 4.62DD289 pKa = 3.86MSTMGLYY296 pKa = 10.32LVYY299 pKa = 10.49PEE301 pKa = 4.4EE302 pKa = 4.59NKK304 pKa = 10.93DD305 pKa = 3.83EE306 pKa = 4.27LLFWEE311 pKa = 5.18EE312 pKa = 4.06DD313 pKa = 3.56VQPIGVSFSPCGKK326 pKa = 10.07YY327 pKa = 10.19LAFDD331 pKa = 4.14ADD333 pKa = 3.61TRR335 pKa = 11.84VIEE338 pKa = 4.41NTWSIYY344 pKa = 10.3IYY346 pKa = 10.82DD347 pKa = 3.43VDD349 pKa = 3.97GRR351 pKa = 11.84DD352 pKa = 3.2GKK354 pKa = 11.25LITRR358 pKa = 11.84YY359 pKa = 9.78IYY361 pKa = 10.08DD362 pKa = 3.3SHH364 pKa = 8.39IEE366 pKa = 4.02PFSPSWGIIPP376 pKa = 4.78

Molecular weight: 42.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G9C1A1|A0A1G9C1A1_9CLOT Flagellar operon protein OS=Natronincola ferrireducens OX=393762 GN=SAMN05660472_01330 PE=4 SV=1
MM1 pKa = 7.61ARR3 pKa = 11.84VCDD6 pKa = 3.66VCEE9 pKa = 3.97KK10 pKa = 10.85GKK12 pKa = 11.13VYY14 pKa = 10.67GNQVSHH20 pKa = 6.63SNRR23 pKa = 11.84HH24 pKa = 5.35SKK26 pKa = 8.87RR27 pKa = 11.84TWLPNLRR34 pKa = 11.84RR35 pKa = 11.84VKK37 pKa = 10.58AIIDD41 pKa = 3.74GTPKK45 pKa = 10.52RR46 pKa = 11.84INICTRR52 pKa = 11.84CLRR55 pKa = 11.84SGKK58 pKa = 7.94VEE60 pKa = 3.89RR61 pKa = 11.84AII63 pKa = 4.51

Molecular weight: 7.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2920

0

2920

887233

40

2126

303.8

34.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.386 ± 0.048

0.942 ± 0.016

5.104 ± 0.035

7.764 ± 0.051

4.086 ± 0.037

7.115 ± 0.05

1.799 ± 0.019

9.692 ± 0.043

7.842 ± 0.044

9.477 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.825 ± 0.021

4.958 ± 0.037

3.256 ± 0.02

3.099 ± 0.028

3.837 ± 0.032

5.263 ± 0.032

5.267 ± 0.027

6.789 ± 0.038

0.71 ± 0.014

3.789 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski