Sanxia tombus-like virus 5
Average proteome isoelectric point is 8.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KGI1|A0A1L3KGI1_9VIRU Uncharacterized protein OS=Sanxia tombus-like virus 5 OX=1923389 PE=4 SV=1
MM1 pKa = 7.62 GSNPIARR8 pKa = 11.84 KK9 pKa = 9.41 VICHH13 pKa = 5.45 QSCVNNEE20 pKa = 3.95 IIGLSNRR27 pKa = 11.84 HH28 pKa = 5.51 IVDD31 pKa = 3.55 RR32 pKa = 11.84 SYY34 pKa = 11.34 IKK36 pKa = 10.26 FDD38 pKa = 3.19 RR39 pKa = 11.84 EE40 pKa = 3.8 YY41 pKa = 10.32 FLRR44 pKa = 11.84 MAKK47 pKa = 8.81 ATRR50 pKa = 11.84 QEE52 pKa = 4.22 LVDD55 pKa = 3.57 AAQSEE60 pKa = 4.91 KK61 pKa = 10.63 VSKK64 pKa = 10.97 LNVVYY69 pKa = 9.84 GYY71 pKa = 10.86 SGGKK75 pKa = 8.48 RR76 pKa = 11.84 AMYY79 pKa = 10.17 LRR81 pKa = 11.84 AADD84 pKa = 3.5 VPFRR88 pKa = 11.84 VAHH91 pKa = 5.56 SSITAFVKK99 pKa = 10.33 VEE101 pKa = 3.79 KK102 pKa = 10.53 GYY104 pKa = 11.24 EE105 pKa = 3.84 EE106 pKa = 6.07 DD107 pKa = 3.3 IHH109 pKa = 8.06 AKK111 pKa = 8.67 APRR114 pKa = 11.84 MIQYY118 pKa = 9.84 RR119 pKa = 11.84 SPVYY123 pKa = 10.34 NLNLATYY130 pKa = 8.46 LKK132 pKa = 9.69 PLEE135 pKa = 4.39 HH136 pKa = 6.96 ALYY139 pKa = 11.04 ANLLNEE145 pKa = 4.26 NGVPVIAKK153 pKa = 9.09 GKK155 pKa = 9.07 NPRR158 pKa = 11.84 EE159 pKa = 3.86 RR160 pKa = 11.84 AEE162 pKa = 4.33 HH163 pKa = 6.29 IKK165 pKa = 10.82 ACFDD169 pKa = 3.73 DD170 pKa = 4.4 KK171 pKa = 11.28 PYY173 pKa = 10.96 VLAMDD178 pKa = 4.22 HH179 pKa = 6.9 SKK181 pKa = 10.57 FDD183 pKa = 3.21 STIRR187 pKa = 11.84 VEE189 pKa = 4.23 HH190 pKa = 6.38 LRR192 pKa = 11.84 AEE194 pKa = 4.12 HH195 pKa = 6.71 KK196 pKa = 10.26 IYY198 pKa = 11.14 NRR200 pKa = 11.84 VFKK203 pKa = 11.04 SKK205 pKa = 10.27 FLSKK209 pKa = 10.82 LLDD212 pKa = 3.64 LQIDD216 pKa = 3.56 NRR218 pKa = 11.84 GYY220 pKa = 8.82 TRR222 pKa = 11.84 NGLKK226 pKa = 10.3 YY227 pKa = 9.27 RR228 pKa = 11.84 VRR230 pKa = 11.84 GTRR233 pKa = 11.84 MSGDD237 pKa = 3.5 FNTGLGNSVINYY249 pKa = 8.93 IVLKK253 pKa = 10.96 SFLTMNNIKK262 pKa = 10.28 GHH264 pKa = 6.58 IYY266 pKa = 10.68 LDD268 pKa = 4.16 GDD270 pKa = 3.87 DD271 pKa = 4.45 SLVFIDD277 pKa = 4.71 PKK279 pKa = 11.02 HH280 pKa = 6.59 RR281 pKa = 11.84 DD282 pKa = 2.96 LDD284 pKa = 4.01 FSHH287 pKa = 7.36 FEE289 pKa = 3.79 KK290 pKa = 10.99 CGFEE294 pKa = 4.31 TKK296 pKa = 10.14 RR297 pKa = 11.84 EE298 pKa = 3.98 EE299 pKa = 4.36 SDD301 pKa = 3.58 CLADD305 pKa = 5.03 VDD307 pKa = 4.6 FCQAKK312 pKa = 10.39 YY313 pKa = 9.74 MEE315 pKa = 4.75 STNLMVRR322 pKa = 11.84 NPVRR326 pKa = 11.84 AISRR330 pKa = 11.84 SLVQVRR336 pKa = 11.84 RR337 pKa = 11.84 SAFFRR342 pKa = 11.84 IRR344 pKa = 11.84 AGEE347 pKa = 4.36 GIGMTRR353 pKa = 11.84 THH355 pKa = 6.93 PGVPVLYY362 pKa = 9.62 PLYY365 pKa = 10.34 KK366 pKa = 10.59 QMGVDD371 pKa = 4.0 EE372 pKa = 5.13 KK373 pKa = 11.2 PIFLKK378 pKa = 10.04 WYY380 pKa = 8.7 PLIDD384 pKa = 3.95 DD385 pKa = 4.72 RR386 pKa = 11.84 EE387 pKa = 4.25 VKK389 pKa = 9.18 ITDD392 pKa = 3.4 AARR395 pKa = 11.84 VEE397 pKa = 5.14 FWHH400 pKa = 6.59 LWGISPDD407 pKa = 3.4 EE408 pKa = 3.87 QLALEE413 pKa = 4.27 EE414 pKa = 4.43 SRR416 pKa = 11.84 SCGHH420 pKa = 7.02 CGTLHH425 pKa = 6.14 HH426 pKa = 7.2 VEE428 pKa = 4.79 FLCPSYY434 pKa = 11.37 KK435 pKa = 10.18 NAQSVSTTWSGLGG448 pKa = 3.36
Molecular weight: 51.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.539
IPC2_protein 8.463
IPC_protein 8.346
Toseland 8.814
ProMoST 8.843
Dawson 9.224
Bjellqvist 9.165
Wikipedia 9.37
Rodwell 9.355
Grimsley 9.209
Solomon 9.282
Lehninger 9.238
Nozaki 9.268
DTASelect 9.033
Thurlkill 9.121
EMBOSS 9.355
Sillero 9.311
Patrickios 4.52
IPC_peptide 9.268
IPC2_peptide 7.936
IPC2.peptide.svr19 7.716
Protein with the highest isoelectric point:
>tr|A0A1L3KGI1|A0A1L3KGI1_9VIRU Uncharacterized protein OS=Sanxia tombus-like virus 5 OX=1923389 PE=4 SV=1
MM1 pKa = 8.01 CIRR4 pKa = 11.84 ITMEE8 pKa = 4.61 KK9 pKa = 9.99 INPTKK14 pKa = 10.18 SVRR17 pKa = 11.84 RR18 pKa = 11.84 AGANVTYY25 pKa = 10.34 QRR27 pKa = 11.84 NLQRR31 pKa = 11.84 LTLSPPAKK39 pKa = 10.22 LGVPLGQGPIPFSRR53 pKa = 11.84 LGAVLNSGFLPPADD67 pKa = 3.89 MLQSVSPKK75 pKa = 9.42 RR76 pKa = 11.84 DD77 pKa = 3.1 KK78 pKa = 11.18 PSFKK82 pKa = 10.17 KK83 pKa = 9.76 DD84 pKa = 3.14 TSVQPQATHH93 pKa = 6.44 TDD95 pKa = 2.84 VTAQKK100 pKa = 10.89 KK101 pKa = 5.87 MRR103 pKa = 11.84 KK104 pKa = 9.04 VVITYY109 pKa = 8.93 AAKK112 pKa = 9.68 PAPPPPAPIVTVPAAKK128 pKa = 10.04 PNLATEE134 pKa = 4.13 HH135 pKa = 6.28 MEE137 pKa = 3.9 TRR139 pKa = 11.84 LAGHH143 pKa = 6.41 RR144 pKa = 11.84 QHH146 pKa = 6.31 VVKK149 pKa = 10.76 GRR151 pKa = 11.84 TRR153 pKa = 11.84 DD154 pKa = 3.46 AYY156 pKa = 11.01 CNNIIDD162 pKa = 4.14 KK163 pKa = 11.01 ADD165 pKa = 3.22 IEE167 pKa = 4.5 KK168 pKa = 10.35 PVLHH172 pKa = 6.32 TEE174 pKa = 3.76 EE175 pKa = 5.41 LIYY178 pKa = 10.42 DD179 pKa = 4.17 VKK181 pKa = 10.96 LLKK184 pKa = 10.35 PSFKK188 pKa = 10.62 AIVRR192 pKa = 11.84 TGVARR197 pKa = 11.84 LLRR200 pKa = 11.84 AHH202 pKa = 6.13 GRR204 pKa = 11.84 EE205 pKa = 4.2 AIAVEE210 pKa = 4.21 AEE212 pKa = 4.3 LLGHH216 pKa = 6.72 LQLEE220 pKa = 4.43 AMFQKK225 pKa = 10.56 RR226 pKa = 11.84 SAKK229 pKa = 10.35 LLLLLKK235 pKa = 9.73 TKK237 pKa = 10.57 AIQWLKK243 pKa = 10.62 NFSKK247 pKa = 10.6 IILKK251 pKa = 6.58 TTEE254 pKa = 3.3 IVALVGRR261 pKa = 11.84 TVAAAMCVHH270 pKa = 5.99 EE271 pKa = 4.62 VEE273 pKa = 4.51 EE274 pKa = 4.52 EE275 pKa = 3.75 ARR277 pKa = 11.84 QLLGDD282 pKa = 3.55 QAEE285 pKa = 4.33 TLNRR289 pKa = 11.84 AKK291 pKa = 9.87 HH292 pKa = 5.34 TDD294 pKa = 3.78 FLHH297 pKa = 6.39 GKK299 pKa = 8.84 CKK301 pKa = 10.5 SGLFQSRR308 pKa = 11.84 LAGDD312 pKa = 3.85 DD313 pKa = 3.34
Molecular weight: 34.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.087
IPC2_protein 9.253
IPC_protein 9.209
Toseland 10.335
ProMoST 9.809
Dawson 10.423
Bjellqvist 10.014
Wikipedia 10.526
Rodwell 11.023
Grimsley 10.452
Solomon 10.452
Lehninger 10.438
Nozaki 10.321
DTASelect 9.999
Thurlkill 10.321
EMBOSS 10.701
Sillero 10.335
Patrickios 10.745
IPC_peptide 10.467
IPC2_peptide 8.507
IPC2.peptide.svr19 8.297
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
761
313
448
380.5
42.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.279 ± 1.615
1.708 ± 0.269
4.993 ± 0.725
5.519 ± 0.255
3.679 ± 0.702
5.65 ± 0.337
3.548 ± 0.221
5.519 ± 0.255
7.753 ± 0.746
9.593 ± 0.994
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.365 ± 0.081
4.074 ± 0.749
4.993 ± 1.073
3.154 ± 0.825
7.227 ± 0.324
5.782 ± 1.018
4.862 ± 1.155
7.359 ± 0.007
0.657 ± 0.211
3.285 ± 1.255
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here