Gremmeniella abietina mitochondrial RNA virus S2

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Howeltoviricetes; Cryppavirales; Mitoviridae; Mitovirus; unclassified Mitovirus

Average proteome isoelectric point is 8.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6GYC2|Q6GYC2_9VIRU Putative RNA-dependent RNA polymerase OS=Gremmeniella abietina mitochondrial RNA virus S2 OX=279538 PE=4 SV=1
MM1 pKa = 7.52NKK3 pKa = 9.71NLFQIVIKK11 pKa = 9.89LFVMIFLNTDD21 pKa = 2.77VKK23 pKa = 11.18LFVSLYY29 pKa = 9.06FRR31 pKa = 11.84CLTKK35 pKa = 10.24HH36 pKa = 5.95LKK38 pKa = 9.14HH39 pKa = 6.61HH40 pKa = 6.28GVVSTVKK47 pKa = 9.93IFKK50 pKa = 10.1QIRR53 pKa = 11.84LHH55 pKa = 4.36VTRR58 pKa = 11.84YY59 pKa = 10.24LCGSPLRR66 pKa = 11.84TNSMMIGIDD75 pKa = 3.31KK76 pKa = 10.82DD77 pKa = 3.51GWPKK81 pKa = 10.22MINFLKK87 pKa = 10.49PLANGTRR94 pKa = 11.84SDD96 pKa = 3.6KK97 pKa = 10.91QLLLSILFITRR108 pKa = 11.84SFVEE112 pKa = 3.84KK113 pKa = 10.46DD114 pKa = 3.04KK115 pKa = 11.81SKK117 pKa = 10.89LVPDD121 pKa = 4.48WSTITQPRR129 pKa = 11.84TTQKK133 pKa = 10.9EE134 pKa = 4.14YY135 pKa = 10.1IIPSGFIKK143 pKa = 10.53DD144 pKa = 3.36WVLNNHH150 pKa = 6.82LGTYY154 pKa = 9.91KK155 pKa = 10.54SEE157 pKa = 4.4FDD159 pKa = 3.96DD160 pKa = 4.12SNHH163 pKa = 5.14FVSVKK168 pKa = 10.29SSPTGPSTLTALWGLWNHH186 pKa = 6.21SYY188 pKa = 11.41EE189 pKa = 4.31SFNWLFKK196 pKa = 9.53ITSLSGVDD204 pKa = 3.75YY205 pKa = 9.4LTKK208 pKa = 10.42IMNWTWLKK216 pKa = 11.21DD217 pKa = 3.63LGNKK221 pKa = 9.13NNNKK225 pKa = 8.74WSKK228 pKa = 10.59QFLGSLSLIYY238 pKa = 10.62DD239 pKa = 3.93PEE241 pKa = 4.54CKK243 pKa = 10.22VRR245 pKa = 11.84IVAMLDD251 pKa = 3.22YY252 pKa = 7.64TTQLFLRR259 pKa = 11.84PIHH262 pKa = 6.62NDD264 pKa = 2.79LFKK267 pKa = 11.04LLKK270 pKa = 10.24KK271 pKa = 10.48LPQDD275 pKa = 3.19RR276 pKa = 11.84TFTQNPLNDD285 pKa = 3.74WEE287 pKa = 4.43NNEE290 pKa = 3.99HH291 pKa = 6.22SFWSIDD297 pKa = 3.21LTAATDD303 pKa = 3.84RR304 pKa = 11.84FPISLQRR311 pKa = 11.84RR312 pKa = 11.84LLLYY316 pKa = 9.94IYY318 pKa = 10.2SDD320 pKa = 3.92PEE322 pKa = 4.35IANSWQNLLVHH333 pKa = 6.78RR334 pKa = 11.84EE335 pKa = 3.82YY336 pKa = 11.34ARR338 pKa = 11.84NGLNPIKK345 pKa = 10.58YY346 pKa = 10.08SVGQPMGAYY355 pKa = 9.9SSWPAFTLSHH365 pKa = 6.52HH366 pKa = 6.85LVVHH370 pKa = 6.07WCAHH374 pKa = 5.74LCHH377 pKa = 6.54INKK380 pKa = 9.75FKK382 pKa = 10.98DD383 pKa = 3.71YY384 pKa = 10.56IILGDD389 pKa = 5.2DD390 pKa = 3.44IVIHH394 pKa = 6.21NDD396 pKa = 2.92KK397 pKa = 10.52VAKK400 pKa = 9.92KK401 pKa = 9.9YY402 pKa = 10.53IEE404 pKa = 3.94IMGKK408 pKa = 10.23LGVGLSDD415 pKa = 4.03SKK417 pKa = 9.8THH419 pKa = 5.62VSKK422 pKa = 10.14DD423 pKa = 3.03TYY425 pKa = 10.9EE426 pKa = 3.91FAKK429 pKa = 10.33RR430 pKa = 11.84WIHH433 pKa = 6.26KK434 pKa = 8.89GQEE437 pKa = 3.85ISPLPITGIVNNITNPYY454 pKa = 8.12IVLMNLYY461 pKa = 10.5DD462 pKa = 3.65FFKK465 pKa = 10.49IKK467 pKa = 10.31RR468 pKa = 11.84NQYY471 pKa = 9.74NFSGDD476 pKa = 3.69ILTMLLKK483 pKa = 10.53LYY485 pKa = 10.43QGLKK489 pKa = 9.4IYY491 pKa = 10.73KK492 pKa = 8.87GDD494 pKa = 3.59KK495 pKa = 9.92SKK497 pKa = 10.87KK498 pKa = 9.53HH499 pKa = 6.09YY500 pKa = 9.99YY501 pKa = 10.3LSLNRR506 pKa = 11.84FKK508 pKa = 11.28NLDD511 pKa = 3.18IFLFALNNTFGYY523 pKa = 8.19STYY526 pKa = 10.08DD527 pKa = 3.05HH528 pKa = 6.81KK529 pKa = 11.28RR530 pKa = 11.84SFLISKK536 pKa = 9.01EE537 pKa = 3.94YY538 pKa = 11.08SKK540 pKa = 11.04DD541 pKa = 3.51LPGPKK546 pKa = 9.19IMQDD550 pKa = 3.83LIXYY554 pKa = 7.52VISTGLSNLTVEE566 pKa = 5.03SVNKK570 pKa = 10.0SLKK573 pKa = 10.53FVDD576 pKa = 4.48TIIDD580 pKa = 3.69NKK582 pKa = 10.89SLLNIEE588 pKa = 5.04DD589 pKa = 4.53PNDD592 pKa = 3.82LSNLPLFKK600 pKa = 10.71GFQAYY605 pKa = 9.56ISNLRR610 pKa = 11.84KK611 pKa = 7.8TVEE614 pKa = 3.54GWNNKK619 pKa = 9.67SISLIEE625 pKa = 3.89ASRR628 pKa = 11.84GLVQMDD634 pKa = 4.49ILPIMKK640 pKa = 10.13GSNRR644 pKa = 11.84KK645 pKa = 8.84ILEE648 pKa = 4.04LQTIGKK654 pKa = 9.36IYY656 pKa = 10.3QNGIQEE662 pKa = 4.19INNTDD667 pKa = 3.86FEE669 pKa = 4.95IFGSSQMIHH678 pKa = 6.53PNEE681 pKa = 3.94SLGIQEE687 pKa = 4.49GSSLNSFSSLSSSIDD702 pKa = 3.36TNLVFIQNEE711 pKa = 4.12LNVIAKK717 pKa = 9.44KK718 pKa = 10.73NWMPPATEE726 pKa = 4.84DD727 pKa = 4.39SVADD731 pKa = 3.8SYY733 pKa = 12.19AAFAAMFNN741 pKa = 3.95

Molecular weight: 85.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6GYC2|Q6GYC2_9VIRU Putative RNA-dependent RNA polymerase OS=Gremmeniella abietina mitochondrial RNA virus S2 OX=279538 PE=4 SV=1
MM1 pKa = 7.52NKK3 pKa = 9.71NLFQIVIKK11 pKa = 9.89LFVMIFLNTDD21 pKa = 2.77VKK23 pKa = 11.18LFVSLYY29 pKa = 9.06FRR31 pKa = 11.84CLTKK35 pKa = 10.24HH36 pKa = 5.95LKK38 pKa = 9.14HH39 pKa = 6.61HH40 pKa = 6.28GVVSTVKK47 pKa = 9.93IFKK50 pKa = 10.1QIRR53 pKa = 11.84LHH55 pKa = 4.36VTRR58 pKa = 11.84YY59 pKa = 10.24LCGSPLRR66 pKa = 11.84TNSMMIGIDD75 pKa = 3.31KK76 pKa = 10.82DD77 pKa = 3.51GWPKK81 pKa = 10.22MINFLKK87 pKa = 10.49PLANGTRR94 pKa = 11.84SDD96 pKa = 3.6KK97 pKa = 10.91QLLLSILFITRR108 pKa = 11.84SFVEE112 pKa = 3.84KK113 pKa = 10.46DD114 pKa = 3.04KK115 pKa = 11.81SKK117 pKa = 10.89LVPDD121 pKa = 4.48WSTITQPRR129 pKa = 11.84TTQKK133 pKa = 10.9EE134 pKa = 4.14YY135 pKa = 10.1IIPSGFIKK143 pKa = 10.53DD144 pKa = 3.36WVLNNHH150 pKa = 6.82LGTYY154 pKa = 9.91KK155 pKa = 10.54SEE157 pKa = 4.4FDD159 pKa = 3.96DD160 pKa = 4.12SNHH163 pKa = 5.14FVSVKK168 pKa = 10.29SSPTGPSTLTALWGLWNHH186 pKa = 6.21SYY188 pKa = 11.41EE189 pKa = 4.31SFNWLFKK196 pKa = 9.53ITSLSGVDD204 pKa = 3.75YY205 pKa = 9.4LTKK208 pKa = 10.42IMNWTWLKK216 pKa = 11.21DD217 pKa = 3.63LGNKK221 pKa = 9.13NNNKK225 pKa = 8.74WSKK228 pKa = 10.59QFLGSLSLIYY238 pKa = 10.62DD239 pKa = 3.93PEE241 pKa = 4.54CKK243 pKa = 10.22VRR245 pKa = 11.84IVAMLDD251 pKa = 3.22YY252 pKa = 7.64TTQLFLRR259 pKa = 11.84PIHH262 pKa = 6.62NDD264 pKa = 2.79LFKK267 pKa = 11.04LLKK270 pKa = 10.24KK271 pKa = 10.48LPQDD275 pKa = 3.19RR276 pKa = 11.84TFTQNPLNDD285 pKa = 3.74WEE287 pKa = 4.43NNEE290 pKa = 3.99HH291 pKa = 6.22SFWSIDD297 pKa = 3.21LTAATDD303 pKa = 3.84RR304 pKa = 11.84FPISLQRR311 pKa = 11.84RR312 pKa = 11.84LLLYY316 pKa = 9.94IYY318 pKa = 10.2SDD320 pKa = 3.92PEE322 pKa = 4.35IANSWQNLLVHH333 pKa = 6.78RR334 pKa = 11.84EE335 pKa = 3.82YY336 pKa = 11.34ARR338 pKa = 11.84NGLNPIKK345 pKa = 10.58YY346 pKa = 10.08SVGQPMGAYY355 pKa = 9.9SSWPAFTLSHH365 pKa = 6.52HH366 pKa = 6.85LVVHH370 pKa = 6.07WCAHH374 pKa = 5.74LCHH377 pKa = 6.54INKK380 pKa = 9.75FKK382 pKa = 10.98DD383 pKa = 3.71YY384 pKa = 10.56IILGDD389 pKa = 5.2DD390 pKa = 3.44IVIHH394 pKa = 6.21NDD396 pKa = 2.92KK397 pKa = 10.52VAKK400 pKa = 9.92KK401 pKa = 9.9YY402 pKa = 10.53IEE404 pKa = 3.94IMGKK408 pKa = 10.23LGVGLSDD415 pKa = 4.03SKK417 pKa = 9.8THH419 pKa = 5.62VSKK422 pKa = 10.14DD423 pKa = 3.03TYY425 pKa = 10.9EE426 pKa = 3.91FAKK429 pKa = 10.33RR430 pKa = 11.84WIHH433 pKa = 6.26KK434 pKa = 8.89GQEE437 pKa = 3.85ISPLPITGIVNNITNPYY454 pKa = 8.12IVLMNLYY461 pKa = 10.5DD462 pKa = 3.65FFKK465 pKa = 10.49IKK467 pKa = 10.31RR468 pKa = 11.84NQYY471 pKa = 9.74NFSGDD476 pKa = 3.69ILTMLLKK483 pKa = 10.53LYY485 pKa = 10.43QGLKK489 pKa = 9.4IYY491 pKa = 10.73KK492 pKa = 8.87GDD494 pKa = 3.59KK495 pKa = 9.92SKK497 pKa = 10.87KK498 pKa = 9.53HH499 pKa = 6.09YY500 pKa = 9.99YY501 pKa = 10.3LSLNRR506 pKa = 11.84FKK508 pKa = 11.28NLDD511 pKa = 3.18IFLFALNNTFGYY523 pKa = 8.19STYY526 pKa = 10.08DD527 pKa = 3.05HH528 pKa = 6.81KK529 pKa = 11.28RR530 pKa = 11.84SFLISKK536 pKa = 9.01EE537 pKa = 3.94YY538 pKa = 11.08SKK540 pKa = 11.04DD541 pKa = 3.51LPGPKK546 pKa = 9.19IMQDD550 pKa = 3.83LIXYY554 pKa = 7.52VISTGLSNLTVEE566 pKa = 5.03SVNKK570 pKa = 10.0SLKK573 pKa = 10.53FVDD576 pKa = 4.48TIIDD580 pKa = 3.69NKK582 pKa = 10.89SLLNIEE588 pKa = 5.04DD589 pKa = 4.53PNDD592 pKa = 3.82LSNLPLFKK600 pKa = 10.71GFQAYY605 pKa = 9.56ISNLRR610 pKa = 11.84KK611 pKa = 7.8TVEE614 pKa = 3.54GWNNKK619 pKa = 9.67SISLIEE625 pKa = 3.89ASRR628 pKa = 11.84GLVQMDD634 pKa = 4.49ILPIMKK640 pKa = 10.13GSNRR644 pKa = 11.84KK645 pKa = 8.84ILEE648 pKa = 4.04LQTIGKK654 pKa = 9.36IYY656 pKa = 10.3QNGIQEE662 pKa = 4.19INNTDD667 pKa = 3.86FEE669 pKa = 4.95IFGSSQMIHH678 pKa = 6.53PNEE681 pKa = 3.94SLGIQEE687 pKa = 4.49GSSLNSFSSLSSSIDD702 pKa = 3.36TNLVFIQNEE711 pKa = 4.12LNVIAKK717 pKa = 9.44KK718 pKa = 10.73NWMPPATEE726 pKa = 4.84DD727 pKa = 4.39SVADD731 pKa = 3.8SYY733 pKa = 12.19AAFAAMFNN741 pKa = 3.95

Molecular weight: 85.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

741

741

741

741.0

85.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.969 ± 0.0

0.675 ± 0.0

5.533 ± 0.0

3.239 ± 0.0

5.398 ± 0.0

4.858 ± 0.0

2.834 ± 0.0

8.637 ± 0.0

8.502 ± 0.0

11.741 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.294 ± 0.0

7.692 ± 0.0

3.779 ± 0.0

3.239 ± 0.0

2.969 ± 0.0

9.042 ± 0.0

5.398 ± 0.0

4.723 ± 0.0

2.294 ± 0.0

4.049 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski