Arthrobacter sp. HMWF013

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter; unclassified Arthrobacter

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4431 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R7QNQ7|A0A2R7QNQ7_9MICC Uncharacterized protein OS=Arthrobacter sp. HMWF013 OX=2056849 GN=DBR22_20945 PE=4 SV=1
MM1 pKa = 6.43TTDD4 pKa = 3.95YY5 pKa = 11.39YY6 pKa = 11.42DD7 pKa = 3.91GLPAVTAGDD16 pKa = 4.02GSPYY20 pKa = 10.32SPSLRR25 pKa = 11.84EE26 pKa = 3.29ISVRR30 pKa = 11.84IRR32 pKa = 11.84DD33 pKa = 3.92LMVLLDD39 pKa = 3.58EE40 pKa = 4.91CLIGSAQTSEE50 pKa = 4.62DD51 pKa = 3.6LSALAWISQSLEE63 pKa = 4.31DD64 pKa = 4.77IEE66 pKa = 4.49ATVTGLTHH74 pKa = 7.54GEE76 pKa = 4.0YY77 pKa = 10.15QQLTDD82 pKa = 4.18DD83 pKa = 4.82RR84 pKa = 11.84SPAVPVYY91 pKa = 10.46QLTEE95 pKa = 3.88QGSVLISS102 pKa = 3.28

Molecular weight: 11.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R7RH40|A0A2R7RH40_9MICC Probable membrane transporter protein OS=Arthrobacter sp. HMWF013 OX=2056849 GN=DBR22_01970 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4431

0

4431

1293161

24

1637

291.8

31.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.837 ± 0.047

0.601 ± 0.008

5.629 ± 0.025

5.654 ± 0.035

3.315 ± 0.026

9.135 ± 0.036

2.094 ± 0.017

4.458 ± 0.029

2.853 ± 0.028

10.223 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.018 ± 0.014

2.539 ± 0.02

5.401 ± 0.026

3.166 ± 0.018

6.344 ± 0.033

5.919 ± 0.022

5.938 ± 0.026

8.253 ± 0.032

1.463 ± 0.016

2.156 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski