Wheat stripe mosaic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Hepelivirales; Benyviridae; unclassified Benyviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386GYE2|A0A386GYE2_9VIRU TGB2 OS=Wheat stripe mosaic virus OX=2364281 PE=4 SV=1
MM1 pKa = 7.66CGFGGIIEE9 pKa = 4.38VCGYY13 pKa = 7.29LHH15 pKa = 7.66CEE17 pKa = 4.14TLWLSVKK24 pKa = 9.5ATT26 pKa = 3.52

Molecular weight: 2.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386GYE2|A0A386GYE2_9VIRU TGB2 OS=Wheat stripe mosaic virus OX=2364281 PE=4 SV=1
MM1 pKa = 7.67SDD3 pKa = 2.79KK4 pKa = 10.77DD5 pKa = 3.52YY6 pKa = 11.2RR7 pKa = 11.84FNGGGGGRR15 pKa = 11.84GLAQKK20 pKa = 10.71GDD22 pKa = 3.52QTLWICVVAAVVIMCGMFVLGPNKK46 pKa = 9.94HH47 pKa = 5.47RR48 pKa = 11.84THH50 pKa = 6.41SAGDD54 pKa = 3.47YY55 pKa = 9.82GVPTFANGGSYY66 pKa = 10.8KK67 pKa = 10.75DD68 pKa = 3.66GTRR71 pKa = 11.84QATFNKK77 pKa = 8.82ASNMAYY83 pKa = 9.95GGHH86 pKa = 6.18GSKK89 pKa = 10.97GLTNGFILAIVVCICWLAACWFLPKK114 pKa = 10.37PEE116 pKa = 4.91DD117 pKa = 3.67EE118 pKa = 5.59CDD120 pKa = 3.67GACCPVRR127 pKa = 11.84GGGGCC132 pKa = 3.55

Molecular weight: 13.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3742

26

2043

534.6

59.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.952 ± 0.294

1.657 ± 0.675

5.772 ± 0.42

5.933 ± 0.665

4.303 ± 0.609

7.563 ± 2.225

2.565 ± 0.136

3.688 ± 0.48

4.837 ± 0.886

9.327 ± 0.845

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.833 ± 0.289

3.234 ± 0.324

4.356 ± 0.434

3.768 ± 0.49

6.2 ± 0.873

6.387 ± 0.781

5.746 ± 0.26

8.605 ± 0.984

1.256 ± 0.358

2.993 ± 0.358
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski