Tortoise microvirus 56
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W9Y2|A0A4P8W9Y2_9VIRU Uncharacterized protein OS=Tortoise microvirus 56 OX=2583161 PE=4 SV=1
MM1 pKa = 7.68 LSLEE5 pKa = 4.12 LSSWNIDD12 pKa = 3.33 LDD14 pKa = 4.02 CFYY17 pKa = 11.21 DD18 pKa = 4.91 DD19 pKa = 4.63 EE20 pKa = 6.26 GLLFDD25 pKa = 3.75 YY26 pKa = 10.83 YY27 pKa = 11.08 KK28 pKa = 11.03 YY29 pKa = 10.53 RR30 pKa = 11.84 DD31 pKa = 3.99 YY32 pKa = 10.15 EE33 pKa = 4.07 TAMQQQSFFKK43 pKa = 10.22 SYY45 pKa = 11.14 SDD47 pKa = 4.95 AVLDD51 pKa = 5.19 DD52 pKa = 4.57 NMLFDD57 pKa = 6.36 KK58 pKa = 10.45 IRR60 pKa = 11.84 KK61 pKa = 8.28 RR62 pKa = 11.84 NEE64 pKa = 3.35 VEE66 pKa = 4.99 LFSEE70 pKa = 5.64 CYY72 pKa = 9.98
Molecular weight: 8.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.798
IPC2_protein 4.024
IPC_protein 3.961
Toseland 3.757
ProMoST 4.126
Dawson 3.948
Bjellqvist 4.113
Wikipedia 3.897
Rodwell 3.795
Grimsley 3.668
Solomon 3.935
Lehninger 3.897
Nozaki 4.075
DTASelect 4.291
Thurlkill 3.808
EMBOSS 3.897
Sillero 4.075
Patrickios 0.896
IPC_peptide 3.935
IPC2_peptide 4.062
IPC2.peptide.svr19 3.985
Protein with the highest isoelectric point:
>tr|A0A4P8W6E5|A0A4P8W6E5_9VIRU Uncharacterized protein OS=Tortoise microvirus 56 OX=2583161 PE=4 SV=1
MM1 pKa = 7.64 LLHH4 pKa = 6.67 SGLVISILIVIKK16 pKa = 9.74 QQSFIIIEE24 pKa = 4.29 TVEE27 pKa = 3.93 VNIPAGEE34 pKa = 4.52 FKK36 pKa = 10.6 RR37 pKa = 11.84 QQSYY41 pKa = 10.17 SVTRR45 pKa = 11.84 KK46 pKa = 9.09 VMYY49 pKa = 10.15 IKK51 pKa = 10.58 RR52 pKa = 11.84 QGSQKK57 pKa = 9.88 FRR59 pKa = 11.84 VEE61 pKa = 3.84 INEE64 pKa = 4.84 LIHH67 pKa = 7.85 IRR69 pKa = 11.84 DD70 pKa = 3.89 FKK72 pKa = 9.6 TVKK75 pKa = 10.08 IVDD78 pKa = 4.78 EE79 pKa = 4.35 IRR81 pKa = 11.84 LTPNTLRR88 pKa = 11.84 TTNYY92 pKa = 9.41 INLFIQFHH100 pKa = 5.57 IVIRR104 pKa = 11.84 MHH106 pKa = 7.04 VIINTKK112 pKa = 10.15 KK113 pKa = 10.47 NSTAQQCTRR122 pKa = 11.84 LRR124 pKa = 11.84 YY125 pKa = 9.76 AEE127 pKa = 3.92 QSLLKK132 pKa = 10.27 SQQTLYY138 pKa = 11.36 VLIGTVRR145 pKa = 11.84 IVAYY149 pKa = 10.28 LIEE152 pKa = 4.3 QMITSYY158 pKa = 11.31 NGLNIVRR165 pKa = 11.84 DD166 pKa = 3.39 IRR168 pKa = 11.84 KK169 pKa = 9.71 NIIIRR174 pKa = 11.84 NVIRR178 pKa = 11.84 KK179 pKa = 9.37 GYY181 pKa = 7.67 STEE184 pKa = 4.05 FNEE187 pKa = 5.79 LRR189 pKa = 11.84 QDD191 pKa = 3.01 WVKK194 pKa = 10.64 FIII197 pKa = 4.47
Molecular weight: 23.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.267
IPC2_protein 9.663
IPC_protein 10.043
Toseland 10.365
ProMoST 10.028
Dawson 10.526
Bjellqvist 10.189
Wikipedia 10.701
Rodwell 10.921
Grimsley 10.584
Solomon 10.57
Lehninger 10.526
Nozaki 10.335
DTASelect 10.189
Thurlkill 10.394
EMBOSS 10.76
Sillero 10.438
Patrickios 10.599
IPC_peptide 10.57
IPC2_peptide 8.902
IPC2.peptide.svr19 8.696
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1716
72
561
245.1
28.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.128 ± 0.873
1.515 ± 0.622
5.245 ± 1.038
4.254 ± 0.97
5.594 ± 1.392
4.487 ± 1.324
1.632 ± 0.402
7.576 ± 1.53
4.021 ± 0.48
9.79 ± 2.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.681 ± 0.33
5.886 ± 1.036
3.497 ± 0.76
4.837 ± 1.398
5.769 ± 0.665
8.974 ± 0.798
5.944 ± 0.742
6.76 ± 0.772
0.816 ± 0.188
5.594 ± 1.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here