Pseudomonas sp. OIL-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3746 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6C0TW04|A0A6C0TW04_9PSED UvrABC system protein C OS=Pseudomonas sp. OIL-1 OX=2706126 GN=uvrC PE=3 SV=1
MM1 pKa = 6.93VPAVALAQPSAGDD14 pKa = 3.42QEE16 pKa = 4.56MTLSGAGSSDD26 pKa = 2.97KK27 pKa = 11.43DD28 pKa = 3.37FDD30 pKa = 4.2ASILSVQGSWGQYY43 pKa = 9.61LSEE46 pKa = 4.28SSLWGIRR53 pKa = 11.84QTVNASDD60 pKa = 4.04SEE62 pKa = 4.63GEE64 pKa = 4.26SVQFDD69 pKa = 3.37GSTRR73 pKa = 11.84VFYY76 pKa = 10.21DD77 pKa = 2.82YY78 pKa = 11.32HH79 pKa = 7.29FGSGNGRR86 pKa = 11.84PFVGVSLGGIYY97 pKa = 10.57GEE99 pKa = 4.47NVDD102 pKa = 4.06EE103 pKa = 4.56TFMGGPEE110 pKa = 3.81VGYY113 pKa = 9.82KK114 pKa = 9.8HH115 pKa = 6.49WLNEE119 pKa = 3.95NTFVTGMVEE128 pKa = 3.97YY129 pKa = 10.81QFLFEE134 pKa = 4.53SASDD138 pKa = 3.39VDD140 pKa = 5.1DD141 pKa = 5.69RR142 pKa = 11.84YY143 pKa = 11.72DD144 pKa = 4.28DD145 pKa = 4.11GALFYY150 pKa = 11.11SVGLGYY156 pKa = 10.93NFF158 pKa = 4.46

Molecular weight: 17.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6C0TNN3|A0A6C0TNN3_9PSED Alkyl hydroperoxide reductase C OS=Pseudomonas sp. OIL-1 OX=2706126 GN=G3M63_05490 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.14QLSAA44 pKa = 3.9

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3746

0

3746

1222925

32

2144

326.5

35.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.504 ± 0.041

0.987 ± 0.014

5.616 ± 0.028

6.235 ± 0.034

3.616 ± 0.024

7.923 ± 0.039

2.284 ± 0.018

5.138 ± 0.028

3.181 ± 0.03

11.256 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.603 ± 0.019

3.023 ± 0.021

4.795 ± 0.024

4.359 ± 0.028

6.596 ± 0.041

5.841 ± 0.026

4.961 ± 0.025

7.108 ± 0.036

1.434 ± 0.016

2.538 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski