Cryphonectria parasitica mycoreovirus 1 (strain 9B21) (CpMYRV-1)
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q65YU9|VP7_MYRV9 Uncharacterized protein VP7 OS=Cryphonectria parasitica mycoreovirus 1 (strain 9B21) OX=230407 GN=S7 PE=4 SV=1
MM1 pKa = 7.92 ADD3 pKa = 3.37 LTSTSVNQTVLATQPLTTRR22 pKa = 11.84 EE23 pKa = 3.97 LQQIRR28 pKa = 11.84 SMVQTSSNLMQFGNHH43 pKa = 4.96 TALPDD48 pKa = 3.6 SYY50 pKa = 11.75 LEE52 pKa = 3.98 TFLFNNGVVYY62 pKa = 10.71 EE63 pKa = 4.95 DD64 pKa = 4.15 GDD66 pKa = 3.79 VFYY69 pKa = 10.7 AWSEE73 pKa = 4.51 TVHH76 pKa = 6.69 HH77 pKa = 6.37 PTFLDD82 pKa = 3.83 AQDD85 pKa = 3.46 QRR87 pKa = 11.84 YY88 pKa = 9.55 SLEE91 pKa = 3.92 LHH93 pKa = 6.82 PIEE96 pKa = 5.54 HH97 pKa = 7.22 DD98 pKa = 3.32 QTVSPNVSHH107 pKa = 6.49 SVWWQCADD115 pKa = 3.39 FNKK118 pKa = 9.67 FRR120 pKa = 11.84 TLSVFGRR127 pKa = 11.84 RR128 pKa = 11.84 VVPRR132 pKa = 11.84 VRR134 pKa = 11.84 YY135 pKa = 9.12 HH136 pKa = 6.88 VDD138 pKa = 2.62 EE139 pKa = 4.34 FTITFSVSVPLRR151 pKa = 11.84 CRR153 pKa = 11.84 SFQDD157 pKa = 3.55 YY158 pKa = 11.24 YY159 pKa = 11.2 DD160 pKa = 3.21 IHH162 pKa = 7.96 VQMKK166 pKa = 8.74 MRR168 pKa = 11.84 TPSPSVHH175 pKa = 7.02 DD176 pKa = 3.8 PTHH179 pKa = 6.4 ISLASEE185 pKa = 4.16 IEE187 pKa = 4.47 SISNDD192 pKa = 2.88 NDD194 pKa = 3.18 VSVPAVVSQPNEE206 pKa = 3.28 ITINVGALTPYY217 pKa = 9.91 VGHH220 pKa = 6.3 SSKK223 pKa = 10.83 LAGAALSMIMNIPRR237 pKa = 11.84 KK238 pKa = 8.96 PVPASPAPRR247 pKa = 11.84 VHH249 pKa = 6.43 PAVLLTHH256 pKa = 6.51 SFFDD260 pKa = 4.04 FVRR263 pKa = 11.84 EE264 pKa = 4.01 QISRR268 pKa = 11.84 MDD270 pKa = 3.52 ATIMPIHH277 pKa = 6.48 LVEE280 pKa = 4.06 QTFPEE285 pKa = 4.41 AFLAIPVPRR294 pKa = 11.84 DD295 pKa = 2.98 IEE297 pKa = 4.43 TGLVTALRR305 pKa = 11.84 SVAMVPTSQYY315 pKa = 9.52 NLHH318 pKa = 6.42 VMAEE322 pKa = 4.35 RR323 pKa = 11.84 VQSQSMDD330 pKa = 2.73 WTGGNFSRR338 pKa = 11.84 IPMHH342 pKa = 7.49 DD343 pKa = 4.27 DD344 pKa = 4.29 DD345 pKa = 7.43 IIMTHH350 pKa = 5.96 DD351 pKa = 4.53 TITALSTGVSTYY363 pKa = 10.01 HH364 pKa = 5.76 HH365 pKa = 6.94 HH366 pKa = 7.23 LAITEE371 pKa = 4.15 DD372 pKa = 3.64 DD373 pKa = 3.57 MAVIKK378 pKa = 10.85 SRR380 pKa = 11.84 VPGVIKK386 pKa = 10.74 YY387 pKa = 10.11 RR388 pKa = 11.84 GSIDD392 pKa = 3.9 DD393 pKa = 5.53 LKK395 pKa = 11.4 ALSNLLEE402 pKa = 4.34 SPRR405 pKa = 11.84 THH407 pKa = 5.89 QVLLHH412 pKa = 6.52 SIATIHH418 pKa = 6.84 IYY420 pKa = 10.55 DD421 pKa = 4.14 IGDD424 pKa = 3.31 EE425 pKa = 4.35 SEE427 pKa = 4.92 IDD429 pKa = 3.69 DD430 pKa = 4.24 QEE432 pKa = 4.36 MYY434 pKa = 10.5 SKK436 pKa = 10.77 KK437 pKa = 10.25 ISFLFLLAYY446 pKa = 10.85 LMEE449 pKa = 5.03 CVTLPTTLSQGFEE462 pKa = 3.99 PRR464 pKa = 11.84 LPLLPSSRR472 pKa = 11.84 VPFYY476 pKa = 11.02 LAFGVV481 pKa = 3.62
Molecular weight: 54.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.406
IPC2_protein 5.27
IPC_protein 5.321
Toseland 5.741
ProMoST 5.639
Dawson 5.537
Bjellqvist 5.563
Wikipedia 5.525
Rodwell 5.512
Grimsley 5.944
Solomon 5.537
Lehninger 5.525
Nozaki 5.779
DTASelect 5.982
Thurlkill 5.931
EMBOSS 5.906
Sillero 5.881
Patrickios 3.834
IPC_peptide 5.55
IPC2_peptide 5.893
IPC2.peptide.svr19 5.79
Protein with the highest isoelectric point:
>sp|Q65YV1|VP5_MYRV9 Uncharacterized protein VP5 OS=Cryphonectria parasitica mycoreovirus 1 (strain 9B21) OX=230407 GN=S5 PE=4 SV=1
MM1 pKa = 7.61 EE2 pKa = 5.75 LPHH5 pKa = 6.84 DD6 pKa = 4.25 KK7 pKa = 10.78 AWAYY11 pKa = 11.28 LLLTPPPLRR20 pKa = 11.84 DD21 pKa = 3.6 YY22 pKa = 10.6 FIQLPKK28 pKa = 10.27 PATLQLALNRR38 pKa = 11.84 FAAYY42 pKa = 10.21 LEE44 pKa = 4.06 KK45 pKa = 10.66 QYY47 pKa = 11.29 LRR49 pKa = 11.84 DD50 pKa = 3.41 SLYY53 pKa = 9.81 MEE55 pKa = 5.38 KK56 pKa = 10.03 IVIGTHH62 pKa = 4.35 YY63 pKa = 10.36 NVRR66 pKa = 11.84 KK67 pKa = 10.02 NRR69 pKa = 11.84 VLQPRR74 pKa = 11.84 IFVSPSNYY82 pKa = 8.67 TRR84 pKa = 11.84 ILSTFYY90 pKa = 11.06 DD91 pKa = 4.1 HH92 pKa = 7.93 PDD94 pKa = 4.06 LIAEE98 pKa = 4.21 VLPIQHH104 pKa = 7.06 LSKK107 pKa = 10.84 SQLKK111 pKa = 10.57 RR112 pKa = 11.84 GGLTKK117 pKa = 10.68 NVLTSGLPEE126 pKa = 4.18 MNNEE130 pKa = 3.91 QNPYY134 pKa = 11.07 AVFEE138 pKa = 4.61 VNTIDD143 pKa = 5.1 DD144 pKa = 4.59 LKK146 pKa = 11.33 ACFAFPTEE154 pKa = 3.99 WLIDD158 pKa = 3.32 ILTGRR163 pKa = 11.84 NSISLHH169 pKa = 3.96 YY170 pKa = 9.61 TIDD173 pKa = 3.35 RR174 pKa = 11.84 TSIPKK179 pKa = 9.48 WYY181 pKa = 9.98 AHH183 pKa = 6.52 LEE185 pKa = 3.85 ARR187 pKa = 11.84 FQYY190 pKa = 10.91 SDD192 pKa = 3.67 GVTKK196 pKa = 10.81 SLTDD200 pKa = 3.32 ADD202 pKa = 4.39 LRR204 pKa = 11.84 QHH206 pKa = 6.51 YY207 pKa = 10.13 IGVQPIPVTCEE218 pKa = 3.4 EE219 pKa = 5.04 LLQKK223 pKa = 10.51 MNDD226 pKa = 3.07 ASINGNTMYY235 pKa = 10.28 RR236 pKa = 11.84 VHH238 pKa = 7.84 GYY240 pKa = 10.19 EE241 pKa = 4.51 SIVSSRR247 pKa = 11.84 MEE249 pKa = 4.12 TVNDD253 pKa = 3.55 LVKK256 pKa = 11.01 ASGKK260 pKa = 9.83 PYY262 pKa = 10.81 DD263 pKa = 3.92 VVNVQTMSSYY273 pKa = 11.04 SRR275 pKa = 11.84 SISKK279 pKa = 10.48 AINQAVTKK287 pKa = 10.19 DD288 pKa = 3.26 RR289 pKa = 11.84 HH290 pKa = 5.48 NKK292 pKa = 9.12 HH293 pKa = 6.1 VGSTKK298 pKa = 10.48 LKK300 pKa = 9.47 NSQGPRR306 pKa = 11.84 TTIFVMPPYY315 pKa = 9.09 ITVDD319 pKa = 3.74 VVCSPMTIKK328 pKa = 10.66 PGLGPIQTKK337 pKa = 9.16 LLKK340 pKa = 10.66 SIVDD344 pKa = 3.14 QSPRR348 pKa = 11.84 RR349 pKa = 11.84 FRR351 pKa = 11.84 IAYY354 pKa = 7.55 AAKK357 pKa = 9.19 PQIEE361 pKa = 3.87 QDD363 pKa = 3.83 VLNKK367 pKa = 10.36 RR368 pKa = 11.84 IFSTDD373 pKa = 3.44 EE374 pKa = 4.08 GPEE377 pKa = 4.19 SYY379 pKa = 10.48 FQGVEE384 pKa = 4.03 LLTPEE389 pKa = 4.45 AKK391 pKa = 10.25 SLLTALPQLVGTFHH405 pKa = 6.57 YY406 pKa = 9.77 EE407 pKa = 3.83 KK408 pKa = 10.45 LSYY411 pKa = 11.07 AHH413 pKa = 6.42 FPSYY417 pKa = 10.97 RR418 pKa = 11.84 LTAGGYY424 pKa = 8.91 NVDD427 pKa = 3.56 YY428 pKa = 10.14 FKK430 pKa = 11.03 RR431 pKa = 11.84 GVTVIVARR439 pKa = 11.84 KK440 pKa = 9.02 HH441 pKa = 5.39 GGKK444 pKa = 10.76 GMVAKK449 pKa = 9.88 LIWKK453 pKa = 9.66 LGTPVIDD460 pKa = 3.38 SDD462 pKa = 4.32 DD463 pKa = 3.48 YY464 pKa = 11.73 GRR466 pKa = 11.84 IIKK469 pKa = 10.13 LVEE472 pKa = 4.52 AGLTIDD478 pKa = 5.14 DD479 pKa = 4.76 AVTQLFSMDD488 pKa = 3.18 YY489 pKa = 9.86 DD490 pKa = 3.67 QRR492 pKa = 11.84 NSTVSAFDD500 pKa = 3.15 EE501 pKa = 4.37 HH502 pKa = 7.97 MEE504 pKa = 4.28 MIVSQSKK511 pKa = 8.4 MKK513 pKa = 10.73 QEE515 pKa = 3.41 ITYY518 pKa = 9.39 PRR520 pKa = 11.84 ASDD523 pKa = 3.44 PRR525 pKa = 11.84 ITAFADD531 pKa = 4.47 YY532 pKa = 10.13 YY533 pKa = 11.07 SKK535 pKa = 10.86 WFLKK539 pKa = 10.58 VPYY542 pKa = 8.89 SDD544 pKa = 4.18 YY545 pKa = 11.02 VASVKK550 pKa = 10.8 NFVTKK555 pKa = 10.79 NGLSGPNGIQSTNHH569 pKa = 6.2 YY570 pKa = 7.41 DD571 pKa = 3.41 TLVIQVHH578 pKa = 5.63 TLPEE582 pKa = 4.03 ATQFLGVNNIIEE594 pKa = 4.59 VYY596 pKa = 10.12 PIIDD600 pKa = 3.85 SYY602 pKa = 11.65 IGMILRR608 pKa = 11.84 QQTSNTCAEE617 pKa = 4.4 LLLARR622 pKa = 11.84 YY623 pKa = 9.35 YY624 pKa = 10.96 EE625 pKa = 4.76 SIHH628 pKa = 6.39 LRR630 pKa = 11.84 NFDD633 pKa = 3.93 MLPVGVVASTLEE645 pKa = 3.98 ALVGG649 pKa = 3.66
Molecular weight: 73.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.887
IPC2_protein 8.083
IPC_protein 7.995
Toseland 8.302
ProMoST 8.419
Dawson 8.756
Bjellqvist 8.712
Wikipedia 8.902
Rodwell 8.829
Grimsley 8.639
Solomon 8.843
Lehninger 8.799
Nozaki 8.668
DTASelect 8.624
Thurlkill 8.668
EMBOSS 8.887
Sillero 8.858
Patrickios 4.113
IPC_peptide 8.829
IPC2_peptide 7.468
IPC2.peptide.svr19 7.632
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
7268
101
1354
660.7
73.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.302 ± 0.154
1.238 ± 0.22
5.407 ± 0.198
3.949 ± 0.161
4.513 ± 0.274
4.898 ± 0.384
3.261 ± 0.197
6.976 ± 0.284
3.082 ± 0.431
9.439 ± 0.309
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.655 ± 0.27
4.444 ± 0.205
6.288 ± 0.399
3.605 ± 0.343
5.476 ± 0.381
8.847 ± 0.336
7.843 ± 0.293
6.962 ± 0.413
0.757 ± 0.075
4.059 ± 0.296
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here