Free State vervet virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Arnidovirineae; Arteriviridae; Simarterivirinae; Epsilonarterivirus; Sheartevirus; Epsilonarterivirus safriver

Average proteome isoelectric point is 7.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A161D4J7|A0A161D4J7_9NIDO ORF3' protein OS=Free State vervet virus OX=1737586 PE=4 SV=1
MM1 pKa = 6.86VSSWLLHH8 pKa = 6.21ACLSCFFFQCMHH20 pKa = 6.94CSNNNGTNTTDD31 pKa = 3.4QISVPTCFSFPRR43 pKa = 11.84ANFSVHH49 pKa = 5.55LHH51 pKa = 6.15FEE53 pKa = 4.14ALVCRR58 pKa = 11.84TDD60 pKa = 3.51PGTLAPGYY68 pKa = 8.61ITASKK73 pKa = 9.75GGCSSVSSGSGYY85 pKa = 11.0AVDD88 pKa = 4.86RR89 pKa = 11.84KK90 pKa = 10.34LIQYY94 pKa = 7.04PHH96 pKa = 7.29NITADD101 pKa = 3.29FDD103 pKa = 5.01LNNTLDD109 pKa = 3.33QSHH112 pKa = 6.24AHH114 pKa = 6.34LAALLTAVLLYY125 pKa = 10.75DD126 pKa = 4.12PEE128 pKa = 4.85SFGLDD133 pKa = 3.49PNKK136 pKa = 10.43SRR138 pKa = 11.84SFNVSSTDD146 pKa = 3.08ANYY149 pKa = 8.65TFCVNGTVSLPNTTLGSYY167 pKa = 10.5YY168 pKa = 10.37FFNSSTWDD176 pKa = 3.61LYY178 pKa = 10.76VLEE181 pKa = 4.56LFRR184 pKa = 11.84PFVLSLLVLSIAFAA198 pKa = 4.32

Molecular weight: 21.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A159D776|A0A159D776_9NIDO GP2b protein OS=Free State vervet virus OX=1737586 PE=4 SV=1
MM1 pKa = 7.17VSKK4 pKa = 10.61ICTDD8 pKa = 3.57PGYY11 pKa = 7.03TTLAFTLAPIIITMLRR27 pKa = 11.84LFRR30 pKa = 11.84PSLRR34 pKa = 11.84GFFTFIAICCLAYY47 pKa = 10.3AATAFNEE54 pKa = 4.16HH55 pKa = 6.24SLATVFTIAASILTLLWKK73 pKa = 10.46LVTWLIIRR81 pKa = 11.84CRR83 pKa = 11.84LCRR86 pKa = 11.84LGPRR90 pKa = 11.84YY91 pKa = 6.34TTAPSSFVEE100 pKa = 4.65STSGTHH106 pKa = 7.58AIPATTTAVVSRR118 pKa = 11.84RR119 pKa = 11.84QGFTLAQGSLVPDD132 pKa = 3.76VKK134 pKa = 11.26KK135 pKa = 9.79MVLSGRR141 pKa = 11.84VAAKK145 pKa = 10.16KK146 pKa = 10.68GLVTLRR152 pKa = 11.84RR153 pKa = 11.84YY154 pKa = 8.49GWKK157 pKa = 8.92TKK159 pKa = 10.31

Molecular weight: 17.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

5528

59

2075

394.9

43.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.285 ± 0.47

3.491 ± 0.203

4.215 ± 0.604

3.455 ± 0.464

5.065 ± 0.459

6.784 ± 0.619

2.985 ± 0.426

5.083 ± 0.37

3.889 ± 0.561

11.053 ± 0.96

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.429 ± 0.139

3.564 ± 0.494

6.151 ± 0.678

2.75 ± 0.415

4.685 ± 0.277

7.58 ± 0.807

7.037 ± 0.329

7.218 ± 0.582

1.266 ± 0.224

4.016 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski