Marco virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Hapavirus; Marco hapavirus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3R1Q9|A0A0D3R1Q9_9RHAB Uncharacterized protein OS=Marco virus OX=1158190 PE=4 SV=1
MM1 pKa = 7.6GSCCGKK7 pKa = 9.63PSRR10 pKa = 11.84RR11 pKa = 11.84SHH13 pKa = 7.31KK14 pKa = 10.27IILPNVLDD22 pKa = 3.5GHH24 pKa = 6.04QFLTDD29 pKa = 3.28EE30 pKa = 4.24SRR32 pKa = 11.84IYY34 pKa = 9.38LTPQDD39 pKa = 4.64PPVSICSVIGHH50 pKa = 7.28DD51 pKa = 3.77ISHH54 pKa = 6.21NRR56 pKa = 11.84NPSVYY61 pKa = 9.56LNHH64 pKa = 6.06YY65 pKa = 9.09NKK67 pKa = 10.77YY68 pKa = 8.95YY69 pKa = 10.84DD70 pKa = 3.5NYY72 pKa = 10.36QNRR75 pKa = 11.84EE76 pKa = 4.06TIRR79 pKa = 11.84YY80 pKa = 9.0IDD82 pKa = 4.74DD83 pKa = 3.93SDD85 pKa = 5.73DD86 pKa = 3.84DD87 pKa = 5.54VYY89 pKa = 11.82DD90 pKa = 3.71QLAATLKK97 pKa = 10.69

Molecular weight: 11.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3R1U6|A0A0D3R1U6_9RHAB GDP polyribonucleotidyltransferase OS=Marco virus OX=1158190 PE=4 SV=1
MM1 pKa = 6.94YY2 pKa = 10.64LKK4 pKa = 9.82TLVLLCGIGVIQSVTQDD21 pKa = 3.35DD22 pKa = 3.98RR23 pKa = 11.84RR24 pKa = 11.84SKK26 pKa = 10.91INLLPFLKK34 pKa = 10.85LEE36 pKa = 4.12WLDD39 pKa = 4.01DD40 pKa = 3.83LRR42 pKa = 11.84KK43 pKa = 9.14PVQDD47 pKa = 3.23VVMDD51 pKa = 3.81SQAVFKK57 pKa = 10.61EE58 pKa = 3.6IFPNILRR65 pKa = 11.84EE66 pKa = 3.96MQAIFQKK73 pKa = 9.99IDD75 pKa = 3.28RR76 pKa = 11.84EE77 pKa = 4.12ISTKK81 pKa = 10.62LFDD84 pKa = 4.32PSRR87 pKa = 11.84EE88 pKa = 4.05AFQKK92 pKa = 10.29MIEE95 pKa = 4.21GTDD98 pKa = 3.71RR99 pKa = 11.84IIKK102 pKa = 8.09QTDD105 pKa = 3.11EE106 pKa = 4.92LFTKK110 pKa = 10.47KK111 pKa = 10.13IPEE114 pKa = 4.42EE115 pKa = 3.87FNKK118 pKa = 9.46MSRR121 pKa = 11.84EE122 pKa = 3.98IARR125 pKa = 11.84STQEE129 pKa = 3.94SINMYY134 pKa = 10.6KK135 pKa = 10.37RR136 pKa = 11.84FEE138 pKa = 4.32KK139 pKa = 10.67DD140 pKa = 3.12FKK142 pKa = 11.47DD143 pKa = 4.01KK144 pKa = 10.63IIEE147 pKa = 4.64PGSHH151 pKa = 7.19AIGSVRR157 pKa = 11.84QEE159 pKa = 3.74ITSSVLMLKK168 pKa = 10.76NNMSTSISRR177 pKa = 11.84MTWNIGNEE185 pKa = 3.95IKK187 pKa = 10.31SLKK190 pKa = 9.32MGLVNEE196 pKa = 5.21KK197 pKa = 9.99ILSLGDD203 pKa = 3.55QINEE207 pKa = 4.03KK208 pKa = 10.33VIKK211 pKa = 9.64KK212 pKa = 8.15TDD214 pKa = 3.32QVLTIFKK221 pKa = 8.77DD222 pKa = 3.4TLNNKK227 pKa = 8.69VSPLFQKK234 pKa = 10.82LSQIDD239 pKa = 3.62FKK241 pKa = 11.71GSVDD245 pKa = 4.15QVNQLIAATKK255 pKa = 9.13GNVVTPVTVLIQSVKK270 pKa = 10.39QLIEE274 pKa = 4.26NYY276 pKa = 10.02SKK278 pKa = 11.1SINNYY283 pKa = 10.03LMPTATKK290 pKa = 10.42ILDD293 pKa = 3.6QVSDD297 pKa = 3.63GTKK300 pKa = 9.53TVFDD304 pKa = 4.5LMSTSSKK311 pKa = 10.2KK312 pKa = 9.67VHH314 pKa = 6.75DD315 pKa = 5.53CILEE319 pKa = 4.07IQASLSTKK327 pKa = 9.68VIPVFEE333 pKa = 4.38GFANYY338 pKa = 7.44VTAISVALKK347 pKa = 10.18PVLEE351 pKa = 4.05NWKK354 pKa = 10.74DD355 pKa = 3.68SIEE358 pKa = 3.84QAFYY362 pKa = 10.6IIGYY366 pKa = 9.34NINQVINQFNIYY378 pKa = 10.31FKK380 pKa = 10.64DD381 pKa = 3.57INWNSWIQNTKK392 pKa = 7.0TWITEE397 pKa = 4.16TYY399 pKa = 10.41LSGKK403 pKa = 9.85AALFDD408 pKa = 4.83FIKK411 pKa = 10.72SASEE415 pKa = 3.88YY416 pKa = 9.91SFKK419 pKa = 10.67AIKK422 pKa = 9.59VIKK425 pKa = 10.02IIAIVLTIITLGIVVIWIINKK446 pKa = 9.07VMIISRR452 pKa = 11.84WCCPNNSS459 pKa = 3.47

Molecular weight: 52.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

4301

97

2134

614.4

70.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.022 ± 0.705

1.628 ± 0.234

5.487 ± 0.446

5.929 ± 0.387

4.441 ± 0.233

5.208 ± 0.667

2.232 ± 0.393

8.417 ± 0.869

7.719 ± 0.828

9.579 ± 0.713

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.651 ± 0.196

5.51 ± 0.409

4.743 ± 0.705

3.86 ± 0.299

3.86 ± 0.38

8.603 ± 0.537

5.464 ± 0.177

5.231 ± 0.521

1.883 ± 0.192

3.534 ± 0.329
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski