Gordonia phage Schmidt

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vendettavirus; unclassified Vendettavirus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385E345|A0A385E345_9CAUD Capsid maturation protease OS=Gordonia phage Schmidt OX=2301697 GN=7 PE=4 SV=1
MM1 pKa = 7.88SGIDD5 pKa = 3.4PEE7 pKa = 5.12GYY9 pKa = 10.51ARR11 pKa = 11.84FGGDD15 pKa = 4.3CICGPLYY22 pKa = 10.69SYY24 pKa = 11.34AGIIEE29 pKa = 4.54PGGIAPDD36 pKa = 4.19CPMHH40 pKa = 6.95GDD42 pKa = 3.31PEE44 pKa = 4.64FYY46 pKa = 10.19EE47 pKa = 4.31KK48 pKa = 10.35RR49 pKa = 11.84DD50 pKa = 3.61AAVQAQLLAGPQQTVLRR67 pKa = 11.84PEE69 pKa = 4.19SFVEE73 pKa = 4.44VVPQQ77 pKa = 3.46

Molecular weight: 8.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385E2Q2|A0A385E2Q2_9CAUD Uncharacterized protein OS=Gordonia phage Schmidt OX=2301697 GN=33 PE=4 SV=1
MM1 pKa = 6.95TRR3 pKa = 11.84RR4 pKa = 11.84GAGRR8 pKa = 11.84STRR11 pKa = 11.84RR12 pKa = 11.84WRR14 pKa = 11.84KK15 pKa = 9.2LRR17 pKa = 11.84AEE19 pKa = 3.94FRR21 pKa = 11.84DD22 pKa = 3.65YY23 pKa = 10.8CAQLRR28 pKa = 11.84LPCWLCGQAIDD39 pKa = 4.22YY40 pKa = 9.72RR41 pKa = 11.84LKK43 pKa = 10.74HH44 pKa = 5.65PHH46 pKa = 6.4PEE48 pKa = 3.47AWEE51 pKa = 4.12PDD53 pKa = 3.05HH54 pKa = 6.7YY55 pKa = 10.49KK56 pKa = 9.55TVRR59 pKa = 11.84EE60 pKa = 4.1HH61 pKa = 7.42PEE63 pKa = 3.85LAEE66 pKa = 4.11DD67 pKa = 3.75WANLRR72 pKa = 11.84PSHH75 pKa = 6.09GACNKK80 pKa = 9.87ARR82 pKa = 11.84GDD84 pKa = 3.78RR85 pKa = 11.84QPSLGLGRR93 pKa = 11.84QSRR96 pKa = 11.84QWW98 pKa = 3.01

Molecular weight: 11.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

13712

46

1101

180.4

19.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.61 ± 0.51

0.868 ± 0.133

6.68 ± 0.25

6.527 ± 0.288

2.698 ± 0.183

9.583 ± 0.433

2.151 ± 0.185

3.457 ± 0.195

3.501 ± 0.253

7.884 ± 0.246

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.129

2.611 ± 0.19

4.653 ± 0.28

4.099 ± 0.157

7.745 ± 0.456

5.018 ± 0.217

5.915 ± 0.257

7.949 ± 0.283

2.1 ± 0.131

2.625 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski