Amino acid dipepetide frequency for Biostraticola tofi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.558AlaAla: 11.558 ± 0.147	1.094AlaCys: 1.094 ± 0.034	5.623AlaAsp: 5.623 ± 0.074	5.841AlaGlu: 5.841 ± 0.068	3.537AlaPhe: 3.537 ± 0.063	8.698AlaGly: 8.698 ± 0.092	1.876AlaHis: 1.876 ± 0.04	6.042AlaIle: 6.042 ± 0.079	3.386AlaLys: 3.386 ± 0.058	12.22AlaLeu: 12.22 ± 0.128	2.942AlaMet: 2.942 ± 0.049	2.925AlaAsn: 2.925 ± 0.05	3.814AlaPro: 3.814 ± 0.071	4.148AlaGln: 4.148 ± 0.063	6.034AlaArg: 6.034 ± 0.086	5.889AlaSer: 5.889 ± 0.072	4.779AlaThr: 4.779 ± 0.067	6.953AlaVal: 6.953 ± 0.086	1.488AlaTrp: 1.488 ± 0.039	2.273AlaTyr: 2.273 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.951CysAla: 0.951 ± 0.026	0.182CysCys: 0.182 ± 0.014	0.521CysAsp: 0.521 ± 0.022	0.509CysGlu: 0.509 ± 0.019	0.396CysPhe: 0.396 ± 0.019	1.029CysGly: 1.029 ± 0.029	0.358CysHis: 0.358 ± 0.021	0.585CysIle: 0.585 ± 0.022	0.262CysLys: 0.262 ± 0.013	1.138CysLeu: 1.138 ± 0.036	0.233CysMet: 0.233 ± 0.015	0.276CysAsn: 0.276 ± 0.015	0.512CysPro: 0.512 ± 0.022	0.481CysGln: 0.481 ± 0.019	0.748CysArg: 0.748 ± 0.027	0.676CysSer: 0.676 ± 0.024	0.446CysThr: 0.446 ± 0.018	0.622CysVal: 0.622 ± 0.022	0.195CysTrp: 0.195 ± 0.012	0.357CysTyr: 0.357 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	5.057AspAla: 5.057 ± 0.072	0.552AspCys: 0.552 ± 0.026	3.025AspAsp: 3.025 ± 0.061	3.284AspGlu: 3.284 ± 0.059	2.114AspPhe: 2.114 ± 0.048	3.873AspGly: 3.873 ± 0.061	1.098AspHis: 1.098 ± 0.028	3.802AspIle: 3.802 ± 0.062	2.406AspLys: 2.406 ± 0.048	4.829AspLeu: 4.829 ± 0.064	1.353AspMet: 1.353 ± 0.033	2.237AspAsn: 2.237 ± 0.05	2.359AspPro: 2.359 ± 0.045	1.951AspGln: 1.951 ± 0.039	3.095AspArg: 3.095 ± 0.05	3.056AspSer: 3.056 ± 0.053	2.446AspThr: 2.446 ± 0.046	3.582AspVal: 3.582 ± 0.056	0.844AspTrp: 0.844 ± 0.026	1.901AspTyr: 1.901 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	4.943GluAla: 4.943 ± 0.072	0.427GluCys: 0.427 ± 0.019	2.309GluAsp: 2.309 ± 0.05	2.697GluGlu: 2.697 ± 0.061	1.767GluPhe: 1.767 ± 0.036	3.356GluGly: 3.356 ± 0.059	1.364GluHis: 1.364 ± 0.032	3.041GluIle: 3.041 ± 0.059	2.63GluLys: 2.63 ± 0.053	5.527GluLeu: 5.527 ± 0.077	1.542GluMet: 1.542 ± 0.04	2.11GluAsn: 2.11 ± 0.045	2.069GluPro: 2.069 ± 0.043	3.443GluGln: 3.443 ± 0.062	3.637GluArg: 3.637 ± 0.061	2.838GluSer: 2.838 ± 0.043	2.641GluThr: 2.641 ± 0.05	3.355GluVal: 3.355 ± 0.062	0.735GluTrp: 0.735 ± 0.025	1.384GluTyr: 1.384 ± 0.038	0.0GluXaa: 0.0 ± 0.0
Phe	3.628PheAla: 3.628 ± 0.058	0.509PheCys: 0.509 ± 0.021	2.282PheAsp: 2.282 ± 0.044	1.679PheGlu: 1.679 ± 0.039	1.624PhePhe: 1.624 ± 0.047	3.116PheGly: 3.116 ± 0.059	0.84PheHis: 0.84 ± 0.03	2.681PheIle: 2.681 ± 0.055	1.214PheLys: 1.214 ± 0.036	3.301PheLeu: 3.301 ± 0.051	0.947PheMet: 0.947 ± 0.03	1.649PheAsn: 1.649 ± 0.039	1.522PhePro: 1.522 ± 0.035	1.112PheGln: 1.112 ± 0.028	1.869PheArg: 1.869 ± 0.041	3.179PheSer: 3.179 ± 0.054	2.238PheThr: 2.238 ± 0.041	2.267PheVal: 2.267 ± 0.053	0.546PheTrp: 0.546 ± 0.022	1.203PheTyr: 1.203 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	6.36GlyAla: 6.36 ± 0.086	1.008GlyCys: 1.008 ± 0.024	3.717GlyAsp: 3.717 ± 0.062	4.228GlyGlu: 4.228 ± 0.067	3.226GlyPhe: 3.226 ± 0.052	5.594GlyGly: 5.594 ± 0.09	1.787GlyHis: 1.787 ± 0.04	5.216GlyIle: 5.216 ± 0.071	3.684GlyLys: 3.684 ± 0.054	8.118GlyLeu: 8.118 ± 0.097	2.349GlyMet: 2.349 ± 0.048	2.768GlyAsn: 2.768 ± 0.05	2.395GlyPro: 2.395 ± 0.038	3.336GlyGln: 3.336 ± 0.061	4.361GlyArg: 4.361 ± 0.068	4.575GlySer: 4.575 ± 0.061	3.663GlyThr: 3.663 ± 0.065	5.506GlyVal: 5.506 ± 0.08	1.291GlyTrp: 1.291 ± 0.038	2.585GlyTyr: 2.585 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	1.985HisAla: 1.985 ± 0.042	0.35HisCys: 0.35 ± 0.017	1.197HisAsp: 1.197 ± 0.034	1.031HisGlu: 1.031 ± 0.032	1.085HisPhe: 1.085 ± 0.032	1.704HisGly: 1.704 ± 0.037	0.827HisHis: 0.827 ± 0.026	1.446HisIle: 1.446 ± 0.031	0.694HisLys: 0.694 ± 0.024	2.416HisLeu: 2.416 ± 0.046	0.533HisMet: 0.533 ± 0.022	0.818HisAsn: 0.818 ± 0.028	1.405HisPro: 1.405 ± 0.04	1.442HisGln: 1.442 ± 0.037	1.54HisArg: 1.54 ± 0.035	1.299HisSer: 1.299 ± 0.035	1.061HisThr: 1.061 ± 0.026	1.135HisVal: 1.135 ± 0.032	0.479HisTrp: 0.479 ± 0.021	0.926HisTyr: 0.926 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	6.734IleAla: 6.734 ± 0.08	0.66IleCys: 0.66 ± 0.023	3.783IleAsp: 3.783 ± 0.059	3.26IleGlu: 3.26 ± 0.06	2.014IlePhe: 2.014 ± 0.044	4.911IleGly: 4.911 ± 0.072	1.192IleHis: 1.192 ± 0.032	3.744IleIle: 3.744 ± 0.061	2.412IleLys: 2.412 ± 0.055	5.161IleLeu: 5.161 ± 0.075	1.35IleMet: 1.35 ± 0.034	2.566IleAsn: 2.566 ± 0.046	2.713IlePro: 2.713 ± 0.048	1.863IleGln: 1.863 ± 0.037	3.184IleArg: 3.184 ± 0.05	4.208IleSer: 4.208 ± 0.062	3.578IleThr: 3.578 ± 0.048	3.64IleVal: 3.64 ± 0.057	0.643IleTrp: 0.643 ± 0.025	1.562IleTyr: 1.562 ± 0.039	0.0IleXaa: 0.0 ± 0.0
Lys	3.761LysAla: 3.761 ± 0.057	0.215LysCys: 0.215 ± 0.014	1.855LysAsp: 1.855 ± 0.051	1.905LysGlu: 1.905 ± 0.047	0.991LysPhe: 0.991 ± 0.026	2.643LysGly: 2.643 ± 0.049	0.801LysHis: 0.801 ± 0.026	2.275LysIle: 2.275 ± 0.049	1.842LysLys: 1.842 ± 0.046	3.887LysLeu: 3.887 ± 0.066	1.073LysMet: 1.073 ± 0.028	1.473LysAsn: 1.473 ± 0.044	1.987LysPro: 1.987 ± 0.044	1.88LysGln: 1.88 ± 0.045	2.415LysArg: 2.415 ± 0.047	2.223LysSer: 2.223 ± 0.04	2.291LysThr: 2.291 ± 0.041	2.558LysVal: 2.558 ± 0.048	0.387LysTrp: 0.387 ± 0.015	0.966LysTyr: 0.966 ± 0.035	0.0LysXaa: 0.0 ± 0.0
Leu	12.614LeuAla: 12.614 ± 0.139	1.286LeuCys: 1.286 ± 0.032	5.877LeuAsp: 5.877 ± 0.071	5.346LeuGlu: 5.346 ± 0.081	4.207LeuPhe: 4.207 ± 0.067	7.761LeuGly: 7.761 ± 0.096	2.341LeuHis: 2.341 ± 0.044	6.086LeuIle: 6.086 ± 0.081	4.222LeuLys: 4.222 ± 0.066	12.773LeuLeu: 12.773 ± 0.162	3.095LeuMet: 3.095 ± 0.055	4.034LeuAsn: 4.034 ± 0.06	6.139LeuPro: 6.139 ± 0.087	4.314LeuGln: 4.314 ± 0.059	6.271LeuArg: 6.271 ± 0.081	8.082LeuSer: 8.082 ± 0.1	6.557LeuThr: 6.557 ± 0.088	7.306LeuVal: 7.306 ± 0.095	1.394LeuTrp: 1.394 ± 0.038	2.756LeuTyr: 2.756 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	2.937MetAla: 2.937 ± 0.047	0.198MetCys: 0.198 ± 0.014	1.172MetAsp: 1.172 ± 0.031	1.147MetGlu: 1.147 ± 0.032	0.844MetPhe: 0.844 ± 0.027	1.797MetGly: 1.797 ± 0.041	0.517MetHis: 0.517 ± 0.021	1.54MetIle: 1.54 ± 0.047	1.167MetLys: 1.167 ± 0.031	3.238MetLeu: 3.238 ± 0.057	0.828MetMet: 0.828 ± 0.024	1.052MetAsn: 1.052 ± 0.031	1.431MetPro: 1.431 ± 0.037	1.1MetGln: 1.1 ± 0.03	1.447MetArg: 1.447 ± 0.036	1.867MetSer: 1.867 ± 0.038	1.757MetThr: 1.757 ± 0.044	1.934MetVal: 1.934 ± 0.043	0.212MetTrp: 0.212 ± 0.015	0.448MetTyr: 0.448 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.425AsnAla: 3.425 ± 0.057	0.318AsnCys: 0.318 ± 0.015	1.882AsnAsp: 1.882 ± 0.045	1.695AsnGlu: 1.695 ± 0.04	1.179AsnPhe: 1.179 ± 0.035	2.745AsnGly: 2.745 ± 0.044	0.84AsnHis: 0.84 ± 0.024	2.249AsnIle: 2.249 ± 0.05	1.345AsnLys: 1.345 ± 0.037	3.44AsnLeu: 3.44 ± 0.054	0.844AsnMet: 0.844 ± 0.026	1.47AsnAsn: 1.47 ± 0.045	2.076AsnPro: 2.076 ± 0.039	1.662AsnGln: 1.662 ± 0.042	2.145AsnArg: 2.145 ± 0.044	1.992AsnSer: 1.992 ± 0.047	1.829AsnThr: 1.829 ± 0.043	2.138AsnVal: 2.138 ± 0.044	0.504AsnTrp: 0.504 ± 0.021	1.13AsnTyr: 1.13 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	5.457ProAla: 5.457 ± 0.095	0.366ProCys: 0.366 ± 0.017	3.012ProAsp: 3.012 ± 0.051	3.176ProGlu: 3.176 ± 0.057	1.821ProPhe: 1.821 ± 0.04	3.662ProGly: 3.662 ± 0.057	1.098ProHis: 1.098 ± 0.033	2.082ProIle: 2.082 ± 0.041	1.36ProLys: 1.36 ± 0.033	5.589ProLeu: 5.589 ± 0.079	1.1ProMet: 1.1 ± 0.027	1.174ProAsn: 1.174 ± 0.034	1.903ProPro: 1.903 ± 0.044	2.033ProGln: 2.033 ± 0.041	2.152ProArg: 2.152 ± 0.052	2.58ProSer: 2.58 ± 0.043	2.116ProThr: 2.116 ± 0.043	3.816ProVal: 3.816 ± 0.066	0.736ProTrp: 0.736 ± 0.028	1.296ProTyr: 1.296 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	5.17GlnAla: 5.17 ± 0.072	0.397GlnCys: 0.397 ± 0.016	2.016GlnAsp: 2.016 ± 0.039	1.953GlnGlu: 1.953 ± 0.051	1.427GlnPhe: 1.427 ± 0.033	3.457GlnGly: 3.457 ± 0.057	1.318GlnHis: 1.318 ± 0.033	2.211GlnIle: 2.211 ± 0.044	1.544GlnLys: 1.544 ± 0.037	5.287GlnLeu: 5.287 ± 0.08	1.146GlnMet: 1.146 ± 0.031	1.209GlnAsn: 1.209 ± 0.031	2.434GlnPro: 2.434 ± 0.051	3.577GlnGln: 3.577 ± 0.076	3.271GlnArg: 3.271 ± 0.053	2.647GlnSer: 2.647 ± 0.044	2.171GlnThr: 2.171 ± 0.048	3.254GlnVal: 3.254 ± 0.049	0.777GlnTrp: 0.777 ± 0.027	1.12GlnTyr: 1.12 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	4.844ArgAla: 4.844 ± 0.071	0.608ArgCys: 0.608 ± 0.024	3.064ArgAsp: 3.064 ± 0.046	3.397ArgGlu: 3.397 ± 0.06	2.634ArgPhe: 2.634 ± 0.043	3.499ArgGly: 3.499 ± 0.064	1.863ArgHis: 1.863 ± 0.04	3.684ArgIle: 3.684 ± 0.057	2.123ArgLys: 2.123 ± 0.044	7.451ArgLeu: 7.451 ± 0.093	1.635ArgMet: 1.635 ± 0.037	1.955ArgAsn: 1.955 ± 0.044	2.626ArgPro: 2.626 ± 0.046	4.048ArgGln: 4.048 ± 0.065	4.155ArgArg: 4.155 ± 0.065	3.139ArgSer: 3.139 ± 0.06	2.515ArgThr: 2.515 ± 0.042	3.925ArgVal: 3.925 ± 0.065	0.978ArgTrp: 0.978 ± 0.029	2.144ArgTyr: 2.144 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	6.203SerAla: 6.203 ± 0.083	0.587SerCys: 0.587 ± 0.024	3.075SerAsp: 3.075 ± 0.047	2.97SerGlu: 2.97 ± 0.055	2.294SerPhe: 2.294 ± 0.047	5.591SerGly: 5.591 ± 0.079	1.548SerHis: 1.548 ± 0.033	3.27SerIle: 3.27 ± 0.049	1.856SerLys: 1.856 ± 0.045	7.839SerLeu: 7.839 ± 0.09	1.494SerMet: 1.494 ± 0.033	1.865SerAsn: 1.865 ± 0.044	2.904SerPro: 2.904 ± 0.048	2.843SerGln: 2.843 ± 0.054	3.914SerArg: 3.914 ± 0.056	3.743SerSer: 3.743 ± 0.07	2.986SerThr: 2.986 ± 0.049	4.286SerVal: 4.286 ± 0.057	0.964SerTrp: 0.964 ± 0.03	1.702SerTyr: 1.702 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	5.317ThrAla: 5.317 ± 0.075	0.449ThrCys: 0.449 ± 0.02	2.741ThrAsp: 2.741 ± 0.046	2.609ThrGlu: 2.609 ± 0.043	1.911ThrPhe: 1.911 ± 0.036	4.547ThrGly: 4.547 ± 0.072	1.231ThrHis: 1.231 ± 0.032	2.55ThrIle: 2.55 ± 0.048	1.303ThrLys: 1.303 ± 0.038	7.441ThrLeu: 7.441 ± 0.077	1.028ThrMet: 1.028 ± 0.027	1.333ThrAsn: 1.333 ± 0.035	3.064ThrPro: 3.064 ± 0.059	1.938ThrGln: 1.938 ± 0.038	3.091ThrArg: 3.091 ± 0.046	2.748ThrSer: 2.748 ± 0.048	2.598ThrThr: 2.598 ± 0.051	3.795ThrVal: 3.795 ± 0.059	0.687ThrTrp: 0.687 ± 0.023	1.145ThrTyr: 1.145 ± 0.033	0.001ThrXaa: 0.001 ± 0.001
Val	6.829ValAla: 6.829 ± 0.089	0.71ValCys: 0.71 ± 0.027	3.628ValAsp: 3.628 ± 0.059	3.515ValGlu: 3.515 ± 0.067	2.521ValPhe: 2.521 ± 0.047	4.718ValGly: 4.718 ± 0.074	1.188ValHis: 1.188 ± 0.031	4.529ValIle: 4.529 ± 0.065	2.768ValLys: 2.768 ± 0.059	7.213ValLeu: 7.213 ± 0.08	2.165ValMet: 2.165 ± 0.043	2.674ValAsn: 2.674 ± 0.049	3.035ValPro: 3.035 ± 0.051	2.236ValGln: 2.236 ± 0.05	3.675ValArg: 3.675 ± 0.053	4.632ValSer: 4.632 ± 0.06	3.99ValThr: 3.99 ± 0.059	5.164ValVal: 5.164 ± 0.075	0.83ValTrp: 0.83 ± 0.028	1.655ValTyr: 1.655 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	0.971TrpAla: 0.971 ± 0.032	0.175TrpCys: 0.175 ± 0.011	0.589TrpAsp: 0.589 ± 0.023	0.495TrpGlu: 0.495 ± 0.022	0.623TrpPhe: 0.623 ± 0.024	0.875TrpGly: 0.875 ± 0.033	0.477TrpHis: 0.477 ± 0.022	0.71TrpIle: 0.71 ± 0.028	0.42TrpLys: 0.42 ± 0.017	2.301TrpLeu: 2.301 ± 0.058	0.379TrpMet: 0.379 ± 0.018	0.412TrpAsn: 0.412 ± 0.02	0.704TrpPro: 0.704 ± 0.025	1.21TrpGln: 1.21 ± 0.03	1.142TrpArg: 1.142 ± 0.035	0.822TrpSer: 0.822 ± 0.027	0.554TrpThr: 0.554 ± 0.023	0.877TrpVal: 0.877 ± 0.029	0.218TrpTrp: 0.218 ± 0.015	0.402TrpTyr: 0.402 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.375TyrAla: 2.375 ± 0.041	0.384TyrCys: 0.384 ± 0.018	1.497TyrAsp: 1.497 ± 0.037	1.078TyrGlu: 1.078 ± 0.03	1.188TyrPhe: 1.188 ± 0.032	2.19TyrGly: 2.19 ± 0.046	0.808TyrHis: 0.808 ± 0.029	1.433TyrIle: 1.433 ± 0.037	0.789TyrLys: 0.789 ± 0.027	3.291TyrLeu: 3.291 ± 0.053	0.595TyrMet: 0.595 ± 0.022	0.909TyrAsn: 0.909 ± 0.03	1.439TyrPro: 1.439 ± 0.037	1.769TyrGln: 1.769 ± 0.043	2.143TyrArg: 2.143 ± 0.044	1.74TyrSer: 1.74 ± 0.045	1.311TyrThr: 1.311 ± 0.036	1.562TyrVal: 1.562 ± 0.043	0.452TyrTrp: 0.452 ± 0.023	0.928TyrTyr: 0.928 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.002

Statistics based on 3912 proteins (1238576 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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