Streptococcus satellite phage Javan373
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZL52|A0A4D5ZL52_9VIRU Integrase/recombinase OS=Streptococcus satellite phage Javan373 OX=2558665 GN=JavanS373_0001 PE=3 SV=1
MM1 pKa = 7.35 NKK3 pKa = 9.55 IKK5 pKa = 10.52 NLRR8 pKa = 11.84 EE9 pKa = 4.29 SKK11 pKa = 10.73 GLSQDD16 pKa = 3.35 RR17 pKa = 11.84 LSQEE21 pKa = 3.66 LGINLRR27 pKa = 11.84 TLQRR31 pKa = 11.84 WEE33 pKa = 4.09 NGEE36 pKa = 3.77 TAIRR40 pKa = 11.84 SKK42 pKa = 10.88 NINRR46 pKa = 11.84 IADD49 pKa = 3.81 YY50 pKa = 11.07 FNVTVPEE57 pKa = 4.05 LLGYY61 pKa = 10.62 EE62 pKa = 4.06 DD63 pKa = 4.67 FYY65 pKa = 11.41 KK66 pKa = 10.16 IEE68 pKa = 4.24 RR69 pKa = 11.84 EE70 pKa = 4.06 ALEE73 pKa = 4.28 SSSDD77 pKa = 3.26 ISEE80 pKa = 4.38 KK81 pKa = 11.15 LNDD84 pKa = 3.73 PNYY87 pKa = 10.67 NRR89 pKa = 11.84 IILEE93 pKa = 4.07 YY94 pKa = 11.02 NEE96 pKa = 4.42 SNKK99 pKa = 10.3 KK100 pKa = 9.45 NGKK103 pKa = 9.1 FVLLLSTEE111 pKa = 4.01 TDD113 pKa = 3.22 AVPLIEE119 pKa = 4.82 KK120 pKa = 10.23 SIEE123 pKa = 3.99 KK124 pKa = 10.55 VILDD128 pKa = 4.5 NYY130 pKa = 10.82 HH131 pKa = 6.78 KK132 pKa = 10.81 LQDD135 pKa = 3.43 GDD137 pKa = 4.15 YY138 pKa = 9.32 EE139 pKa = 4.21 EE140 pKa = 5.22 SLTGILSEE148 pKa = 5.85 DD149 pKa = 3.06 ITNTYY154 pKa = 9.52 IALSFLPIPFNDD166 pKa = 3.87 FFSKK170 pKa = 10.55 FLTLSNSDD178 pKa = 3.11 KK179 pKa = 11.07 KK180 pKa = 10.87 IVLKK184 pKa = 10.71 LVSSLHH190 pKa = 6.94 DD191 pKa = 3.86 KK192 pKa = 11.25 DD193 pKa = 5.04 DD194 pKa = 3.72 GLGIIKK200 pKa = 8.91 EE201 pKa = 4.19 QNDD204 pKa = 3.94 LNSNTDD210 pKa = 2.88 NN211 pKa = 3.95
Molecular weight: 24.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.857
IPC2_protein 4.825
IPC_protein 4.736
Toseland 4.584
ProMoST 4.838
Dawson 4.673
Bjellqvist 4.825
Wikipedia 4.546
Rodwell 4.571
Grimsley 4.495
Solomon 4.673
Lehninger 4.635
Nozaki 4.787
DTASelect 4.94
Thurlkill 4.584
EMBOSS 4.558
Sillero 4.851
Patrickios 4.228
IPC_peptide 4.685
IPC2_peptide 4.838
IPC2.peptide.svr19 4.849
Protein with the highest isoelectric point:
>tr|A0A4D5ZLD7|A0A4D5ZLD7_9VIRU Major capsid protein OS=Streptococcus satellite phage Javan373 OX=2558665 GN=JavanS373_0008 PE=4 SV=1
MM1 pKa = 7.54 NIKK4 pKa = 8.96 TVVKK8 pKa = 10.72 KK9 pKa = 10.62 NGQTVYY15 pKa = 10.41 RR16 pKa = 11.84 ASVYY20 pKa = 10.77 LGVDD24 pKa = 2.88 QMTGKK29 pKa = 10.55 KK30 pKa = 10.01 SRR32 pKa = 11.84 TTITANTKK40 pKa = 8.53 TAVKK44 pKa = 9.95 IKK46 pKa = 10.44 ARR48 pKa = 11.84 EE49 pKa = 4.34 AINTFANNGYY59 pKa = 8.85 CAKK62 pKa = 10.05 NKK64 pKa = 8.48 PTITTYY70 pKa = 10.95 RR71 pKa = 11.84 EE72 pKa = 4.14 LVALWWEE79 pKa = 4.57 SYY81 pKa = 11.28 KK82 pKa = 10.03 NTIKK86 pKa = 10.78 PNSQQSMEE94 pKa = 4.61 GIVRR98 pKa = 11.84 LHH100 pKa = 6.54 ILPVFGDD107 pKa = 3.69 YY108 pKa = 10.92 KK109 pKa = 10.98 LNSLTTPIIQQQVNKK124 pKa = 9.5 WADD127 pKa = 3.16 KK128 pKa = 10.84 ANRR131 pKa = 11.84 GEE133 pKa = 3.99 KK134 pKa = 9.88 GAYY137 pKa = 9.71 ANYY140 pKa = 10.84 SFLNNINRR148 pKa = 11.84 RR149 pKa = 11.84 ILQYY153 pKa = 10.72 GVTMQVIQHH162 pKa = 5.79 NPARR166 pKa = 11.84 DD167 pKa = 3.51 VIIPRR172 pKa = 11.84 KK173 pKa = 8.85 QQAKK177 pKa = 6.27 EE178 pKa = 3.48 QKK180 pKa = 9.56 IRR182 pKa = 11.84 FFSNQEE188 pKa = 3.74 LKK190 pKa = 10.95 KK191 pKa = 10.46 FLAYY195 pKa = 10.41 LDD197 pKa = 4.4 SLDD200 pKa = 4.03 LNLYY204 pKa = 9.79 EE205 pKa = 5.02 NLFDD209 pKa = 3.74 YY210 pKa = 10.98 VLYY213 pKa = 8.9 KK214 pKa = 10.39 TLLASGCRR222 pKa = 11.84 IGEE225 pKa = 4.0 ALALEE230 pKa = 4.35 WSDD233 pKa = 4.01 INLKK237 pKa = 9.52 TGTISISKK245 pKa = 8.18 TLNRR249 pKa = 11.84 YY250 pKa = 8.7 QEE252 pKa = 4.35 TNTPKK257 pKa = 10.61 SKK259 pKa = 10.87 AGLRR263 pKa = 11.84 DD264 pKa = 3.45 IEE266 pKa = 4.69 IDD268 pKa = 3.36 PATISLLKK276 pKa = 10.13 QYY278 pKa = 10.83 KK279 pKa = 9.51 KK280 pKa = 10.33 RR281 pKa = 11.84 QQVEE285 pKa = 3.92 CWKK288 pKa = 10.56 LGRR291 pKa = 11.84 SEE293 pKa = 5.56 NIVFTPFTTKK303 pKa = 10.34 YY304 pKa = 10.75 AYY306 pKa = 10.29 ACLLRR311 pKa = 11.84 KK312 pKa = 9.66 RR313 pKa = 11.84 LQGHH317 pKa = 6.11 FKK319 pKa = 10.67 SAGVPDD325 pKa = 3.94 ISFHH329 pKa = 5.86 GFRR332 pKa = 11.84 HH333 pKa = 4.4 THH335 pKa = 4.65 ATIMLYY341 pKa = 10.62 AGIEE345 pKa = 4.18 AKK347 pKa = 10.42 DD348 pKa = 3.35 LQYY351 pKa = 11.55 RR352 pKa = 11.84 LGHH355 pKa = 5.94 SNITMTLNTYY365 pKa = 8.72 VHH367 pKa = 6.14 ATKK370 pKa = 10.47 EE371 pKa = 4.25 GAKK374 pKa = 9.7 KK375 pKa = 10.18 AVSIFEE381 pKa = 4.16 TAISNLL387 pKa = 3.59
Molecular weight: 44.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.122
IPC2_protein 9.341
IPC_protein 9.268
Toseland 9.955
ProMoST 9.648
Dawson 10.175
Bjellqvist 9.838
Wikipedia 10.335
Rodwell 10.657
Grimsley 10.248
Solomon 10.189
Lehninger 10.145
Nozaki 9.97
DTASelect 9.838
Thurlkill 10.014
EMBOSS 10.365
Sillero 10.087
Patrickios 10.072
IPC_peptide 10.189
IPC2_peptide 8.419
IPC2.peptide.svr19 8.305
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
2104
48
479
233.8
27.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.658 ± 1.053
0.428 ± 0.177
5.656 ± 0.808
8.65 ± 1.175
4.8 ± 0.563
5.608 ± 0.643
0.998 ± 0.288
7.082 ± 0.703
10.124 ± 0.615
10.266 ± 0.935
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.711 ± 0.404
6.464 ± 0.637
2.757 ± 0.175
3.85 ± 0.611
4.563 ± 0.832
5.656 ± 0.471
5.703 ± 0.736
5.276 ± 0.473
1.378 ± 0.252
4.373 ± 0.483
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here