Vibrio phage Va_90-11-286_p16
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A513SRG6|A0A513SRG6_9CAUD Protease OS=Vibrio phage Va_90-11-286_p16 OX=2565519 PE=3 SV=1
MM1 pKa = 7.51 ILGSVMQLWVLPITLFSAMSYY22 pKa = 9.55 SASNIIPIGYY32 pKa = 7.61 EE33 pKa = 3.72 VPEE36 pKa = 4.26 YY37 pKa = 10.41 IALEE41 pKa = 4.27 EE42 pKa = 4.39 KK43 pKa = 10.96 NSGCEE48 pKa = 4.08 SIVCKK53 pKa = 10.69 DD54 pKa = 3.55 VVEE57 pKa = 4.36 KK58 pKa = 11.05 QSVPKK63 pKa = 10.9 VGDD66 pKa = 3.22 IPKK69 pKa = 10.07 RR70 pKa = 11.84 EE71 pKa = 4.06 GNKK74 pKa = 9.35 TIEE77 pKa = 4.56 TIGSVVYY84 pKa = 9.96 LVGEE88 pKa = 4.39 ASQTNEE94 pKa = 3.86 LPIDD98 pKa = 3.63 EE99 pKa = 5.05 MIEE102 pKa = 4.04 IFSDD106 pKa = 3.29
Molecular weight: 11.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.288
IPC2_protein 4.329
IPC_protein 4.177
Toseland 4.024
ProMoST 4.177
Dawson 4.101
Bjellqvist 4.317
Wikipedia 3.961
Rodwell 4.012
Grimsley 3.935
Solomon 4.088
Lehninger 4.05
Nozaki 4.215
DTASelect 4.291
Thurlkill 4.037
EMBOSS 3.973
Sillero 4.279
Patrickios 3.325
IPC_peptide 4.101
IPC2_peptide 4.266
IPC2.peptide.svr19 4.212
Protein with the highest isoelectric point:
>tr|A0A513SNT1|A0A513SNT1_9CAUD Integrase OS=Vibrio phage Va_90-11-286_p16 OX=2565519 PE=3 SV=1
MM1 pKa = 7.47 GFLRR5 pKa = 11.84 LFVLCCRR12 pKa = 11.84 QMSIFTLKK20 pKa = 11.0 LLVNSNRR27 pKa = 11.84 KK28 pKa = 7.11 TGKK31 pKa = 9.7 FLCLPQLVLALARR44 pKa = 11.84 CQFSGCKK51 pKa = 7.59 VWKK54 pKa = 9.39 GQVNRR59 pKa = 11.84 SFRR62 pKa = 11.84 ICC64 pKa = 3.56
Molecular weight: 7.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.29
IPC2_protein 9.502
IPC_protein 9.75
Toseland 10.862
ProMoST 10.409
Dawson 10.906
Bjellqvist 10.57
Wikipedia 11.038
Rodwell 11.257
Grimsley 10.906
Solomon 11.023
Lehninger 11.008
Nozaki 10.891
DTASelect 10.526
Thurlkill 10.833
EMBOSS 11.257
Sillero 10.847
Patrickios 11.052
IPC_peptide 11.038
IPC2_peptide 10.072
IPC2.peptide.svr19 8.62
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
49
0
49
11544
61
909
235.6
26.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.377 ± 0.61
1.195 ± 0.169
5.449 ± 0.281
6.852 ± 0.324
3.837 ± 0.259
6.272 ± 0.43
2.174 ± 0.227
6.046 ± 0.251
6.67 ± 0.267
9.529 ± 0.261
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.426 ± 0.152
4.479 ± 0.325
3.543 ± 0.22
4.227 ± 0.189
5.57 ± 0.267
7.112 ± 0.394
5.215 ± 0.246
6.679 ± 0.328
1.291 ± 0.15
3.058 ± 0.295
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here