Staphylococcus virus Baq_Sau1 
Average proteome isoelectric point is 6.41 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A6B7H592|A0A6B7H592_9CAUD Uncharacterized protein OS=Staphylococcus virus Baq_Sau1 OX=2562362 PE=4 SV=1MM1 pKa = 7.41  ATQKK5 pKa = 10.18  QVEE8 pKa = 4.59  YY9 pKa = 11.35  VMSLQEE15 pKa = 3.92  QLEE18 pKa = 4.35  LEE20 pKa = 4.45  DD21 pKa = 3.95  CEE23 pKa = 5.56  KK24 pKa = 10.67  YY25 pKa = 9.42  TDD27 pKa = 3.83  EE28 pKa = 4.01  QVKK31 pKa = 10.4  AMSHH35 pKa = 6.03  KK36 pKa = 10.06  EE37 pKa = 3.72  VSNVIEE43 pKa = 4.27  NYY45 pKa = 7.67  KK46 pKa = 9.69  TSIRR50 pKa = 11.84  NEE52 pKa = 3.93  EE53 pKa = 4.27  VYY55 pKa = 10.68  DD56 pKa = 3.56  EE57 pKa = 4.64  CMSFGLPNCC66 pKa = 4.51  
 7.76 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  4.132 
IPC2_protein 4.469 
IPC_protein 4.304 
Toseland    4.164 
ProMoST     4.393 
Dawson      4.228 
Bjellqvist  4.38 
Wikipedia   4.062 
Rodwell     4.139 
Grimsley    4.088 
Solomon     4.215 
Lehninger   4.164 
Nozaki      4.342 
DTASelect   4.406 
Thurlkill   4.164 
EMBOSS      4.088 
Sillero     4.406 
Patrickios  1.964 
IPC_peptide 4.228 
IPC2_peptide  4.393 
IPC2.peptide.svr19  4.328 
 Protein with the highest isoelectric point: 
>tr|A0A6B7H7H7|A0A6B7H7H7_9CAUD Terminase large subunit (Fragment) OS=Staphylococcus virus Baq_Sau1 OX=2562362 PE=4 SV=1MM1 pKa = 7.61  AGIKK5 pKa = 9.47  TKK7 pKa = 10.48  VRR9 pKa = 11.84  IDD11 pKa = 3.68  GKK13 pKa = 10.91  MMMLIDD19 pKa = 5.27  ASDD22 pKa = 3.55  KK23 pKa = 11.18  YY24 pKa = 10.91  DD25 pKa = 3.32  IKK27 pKa = 11.22  VSTLITRR34 pKa = 11.84  YY35 pKa = 10.25  DD36 pKa = 3.02  RR37 pKa = 11.84  GARR40 pKa = 11.84  GKK42 pKa = 10.27  DD43 pKa = 3.3  LIQNVVKK50 pKa = 10.16  PKK52 pKa = 10.1  KK53 pKa = 10.22  VKK55 pKa = 10.48  VDD57 pKa = 3.64  GKK59 pKa = 11.19  LMTVSEE65 pKa = 4.39  MVKK68 pKa = 10.17  KK69 pKa = 10.69  YY70 pKa = 10.62  NLSKK74 pKa = 11.04  GLLNYY79 pKa = 10.28  RR80 pKa = 11.84  IAKK83 pKa = 9.62  GLTGDD88 pKa = 4.42  ALIAPPQEE96 pKa = 4.51  KK97 pKa = 9.94  PPSKK101 pKa = 8.74  YY102 pKa = 8.86  TEE104 pKa = 4.17  YY105 pKa = 10.97  EE106 pKa = 3.78  NEE108 pKa = 3.95  QMKK111 pKa = 10.8  KK112 pKa = 10.27  KK113 pKa = 10.48  GLTPEE118 pKa = 3.71  IVRR121 pKa = 11.84  NRR123 pKa = 11.84  VAKK126 pKa = 10.13  GWEE129 pKa = 3.76  LSEE132 pKa = 6.17  AIDD135 pKa = 4.29  APFGMKK141 pKa = 10.52  LNDD144 pKa = 3.28  YY145 pKa = 10.98  RR146 pKa = 11.84  EE147 pKa = 4.08  IQITKK152 pKa = 8.56  ALEE155 pKa = 4.09  RR156 pKa = 11.84  EE157 pKa = 3.98  RR158 pKa = 11.84  EE159 pKa = 3.89  MARR162 pKa = 11.84  QRR164 pKa = 11.84  RR165 pKa = 11.84  KK166 pKa = 8.97  EE167 pKa = 3.76  AEE169 pKa = 3.84  LRR171 pKa = 11.84  RR172 pKa = 11.84  KK173 pKa = 9.75  KK174 pKa = 10.2  PHH176 pKa = 6.56  LFNVPQKK183 pKa = 10.53  HH184 pKa = 6.08  SRR186 pKa = 11.84  DD187 pKa = 3.98  PYY189 pKa = 10.52  WFDD192 pKa = 2.56  ITYY195 pKa = 10.6  NQMFKK200 pKa = 10.26  KK201 pKa = 9.86  WSEE204 pKa = 3.76  AA205 pKa = 3.32  
 23.95 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.036 
IPC2_protein 9.297 
IPC_protein 9.209 
Toseland    10.16 
ProMoST     9.721 
Dawson      10.292 
Bjellqvist  9.897 
Wikipedia   10.423 
Rodwell     10.906 
Grimsley    10.35 
Solomon     10.321 
Lehninger   10.292 
Nozaki      10.116 
DTASelect   9.897 
Thurlkill   10.16 
EMBOSS      10.54 
Sillero     10.204 
Patrickios  10.584 
IPC_peptide 10.321 
IPC2_peptide  8.273 
IPC2.peptide.svr19  8.332 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        67 
0
67 
13842
48
1155
206.6
23.72
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        5.592 ± 0.391
0.412 ± 0.071
6.307 ± 0.331
7.853 ± 0.619
4.284 ± 0.246
5.454 ± 0.308
1.64 ± 0.145
7.528 ± 0.297
9.471 ± 0.389
7.376 ± 0.296
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.521 ± 0.143
7.022 ± 0.314
2.724 ± 0.212
3.829 ± 0.184
4.067 ± 0.217
5.91 ± 0.241
6.083 ± 0.276
6.206 ± 0.305
1.113 ± 0.192
4.609 ± 0.344
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here