Staphylococcus virus Baq_Sau1
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6B7H592|A0A6B7H592_9CAUD Uncharacterized protein OS=Staphylococcus virus Baq_Sau1 OX=2562362 PE=4 SV=1
MM1 pKa = 7.41 ATQKK5 pKa = 10.18 QVEE8 pKa = 4.59 YY9 pKa = 11.35 VMSLQEE15 pKa = 3.92 QLEE18 pKa = 4.35 LEE20 pKa = 4.45 DD21 pKa = 3.95 CEE23 pKa = 5.56 KK24 pKa = 10.67 YY25 pKa = 9.42 TDD27 pKa = 3.83 EE28 pKa = 4.01 QVKK31 pKa = 10.4 AMSHH35 pKa = 6.03 KK36 pKa = 10.06 EE37 pKa = 3.72 VSNVIEE43 pKa = 4.27 NYY45 pKa = 7.67 KK46 pKa = 9.69 TSIRR50 pKa = 11.84 NEE52 pKa = 3.93 EE53 pKa = 4.27 VYY55 pKa = 10.68 DD56 pKa = 3.56 EE57 pKa = 4.64 CMSFGLPNCC66 pKa = 4.51
Molecular weight: 7.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.132
IPC2_protein 4.469
IPC_protein 4.304
Toseland 4.164
ProMoST 4.393
Dawson 4.228
Bjellqvist 4.38
Wikipedia 4.062
Rodwell 4.139
Grimsley 4.088
Solomon 4.215
Lehninger 4.164
Nozaki 4.342
DTASelect 4.406
Thurlkill 4.164
EMBOSS 4.088
Sillero 4.406
Patrickios 1.964
IPC_peptide 4.228
IPC2_peptide 4.393
IPC2.peptide.svr19 4.328
Protein with the highest isoelectric point:
>tr|A0A6B7H7H7|A0A6B7H7H7_9CAUD Terminase large subunit (Fragment) OS=Staphylococcus virus Baq_Sau1 OX=2562362 PE=4 SV=1
MM1 pKa = 7.61 AGIKK5 pKa = 9.47 TKK7 pKa = 10.48 VRR9 pKa = 11.84 IDD11 pKa = 3.68 GKK13 pKa = 10.91 MMMLIDD19 pKa = 5.27 ASDD22 pKa = 3.55 KK23 pKa = 11.18 YY24 pKa = 10.91 DD25 pKa = 3.32 IKK27 pKa = 11.22 VSTLITRR34 pKa = 11.84 YY35 pKa = 10.25 DD36 pKa = 3.02 RR37 pKa = 11.84 GARR40 pKa = 11.84 GKK42 pKa = 10.27 DD43 pKa = 3.3 LIQNVVKK50 pKa = 10.16 PKK52 pKa = 10.1 KK53 pKa = 10.22 VKK55 pKa = 10.48 VDD57 pKa = 3.64 GKK59 pKa = 11.19 LMTVSEE65 pKa = 4.39 MVKK68 pKa = 10.17 KK69 pKa = 10.69 YY70 pKa = 10.62 NLSKK74 pKa = 11.04 GLLNYY79 pKa = 10.28 RR80 pKa = 11.84 IAKK83 pKa = 9.62 GLTGDD88 pKa = 4.42 ALIAPPQEE96 pKa = 4.51 KK97 pKa = 9.94 PPSKK101 pKa = 8.74 YY102 pKa = 8.86 TEE104 pKa = 4.17 YY105 pKa = 10.97 EE106 pKa = 3.78 NEE108 pKa = 3.95 QMKK111 pKa = 10.8 KK112 pKa = 10.27 KK113 pKa = 10.48 GLTPEE118 pKa = 3.71 IVRR121 pKa = 11.84 NRR123 pKa = 11.84 VAKK126 pKa = 10.13 GWEE129 pKa = 3.76 LSEE132 pKa = 6.17 AIDD135 pKa = 4.29 APFGMKK141 pKa = 10.52 LNDD144 pKa = 3.28 YY145 pKa = 10.98 RR146 pKa = 11.84 EE147 pKa = 4.08 IQITKK152 pKa = 8.56 ALEE155 pKa = 4.09 RR156 pKa = 11.84 EE157 pKa = 3.98 RR158 pKa = 11.84 EE159 pKa = 3.89 MARR162 pKa = 11.84 QRR164 pKa = 11.84 RR165 pKa = 11.84 KK166 pKa = 8.97 EE167 pKa = 3.76 AEE169 pKa = 3.84 LRR171 pKa = 11.84 RR172 pKa = 11.84 KK173 pKa = 9.75 KK174 pKa = 10.2 PHH176 pKa = 6.56 LFNVPQKK183 pKa = 10.53 HH184 pKa = 6.08 SRR186 pKa = 11.84 DD187 pKa = 3.98 PYY189 pKa = 10.52 WFDD192 pKa = 2.56 ITYY195 pKa = 10.6 NQMFKK200 pKa = 10.26 KK201 pKa = 9.86 WSEE204 pKa = 3.76 AA205 pKa = 3.32
Molecular weight: 23.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.036
IPC2_protein 9.297
IPC_protein 9.209
Toseland 10.16
ProMoST 9.721
Dawson 10.292
Bjellqvist 9.897
Wikipedia 10.423
Rodwell 10.906
Grimsley 10.35
Solomon 10.321
Lehninger 10.292
Nozaki 10.116
DTASelect 9.897
Thurlkill 10.16
EMBOSS 10.54
Sillero 10.204
Patrickios 10.584
IPC_peptide 10.321
IPC2_peptide 8.273
IPC2.peptide.svr19 8.332
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
67
0
67
13842
48
1155
206.6
23.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.592 ± 0.391
0.412 ± 0.071
6.307 ± 0.331
7.853 ± 0.619
4.284 ± 0.246
5.454 ± 0.308
1.64 ± 0.145
7.528 ± 0.297
9.471 ± 0.389
7.376 ± 0.296
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.521 ± 0.143
7.022 ± 0.314
2.724 ± 0.212
3.829 ± 0.184
4.067 ± 0.217
5.91 ± 0.241
6.083 ± 0.276
6.206 ± 0.305
1.113 ± 0.192
4.609 ± 0.344
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here