Vibrio phage XM1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A7D7JI10|A0A7D7JI10_9CAUD Pesticin domain-containing protein OS=Vibrio phage XM1 OX=2748688 PE=4 SV=1
MM1 pKa = 7.7ALIDD5 pKa = 3.92DD6 pKa = 5.11FKK8 pKa = 11.72ARR10 pKa = 11.84FPNLDD15 pKa = 3.17GSLVDD20 pKa = 4.31ALVPVYY26 pKa = 10.16EE27 pKa = 4.5NNYY30 pKa = 7.53SCYY33 pKa = 10.52YY34 pKa = 9.13GGSYY38 pKa = 11.0EE39 pKa = 4.04NDD41 pKa = 3.38CDD43 pKa = 4.63KK44 pKa = 11.26EE45 pKa = 5.35AILLLIAHH53 pKa = 6.91LVVTDD58 pKa = 3.95PSYY61 pKa = 11.68SGDD64 pKa = 3.56EE65 pKa = 4.1SSSRR69 pKa = 11.84AVASQSVGSVSVSFVAGSTGSDD91 pKa = 2.65WTNWLNSTRR100 pKa = 11.84YY101 pKa = 8.7GQLFLMVTSNNMGPSFAA118 pKa = 5.17

Molecular weight:
12.74 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A7D7EXM6|A0A7D7EXM6_9CAUD PhageMetallopep domain-containing protein OS=Vibrio phage XM1 OX=2748688 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 10.48KK3 pKa = 9.9EE4 pKa = 3.92LRR6 pKa = 11.84DD7 pKa = 3.99LITKK11 pKa = 9.91KK12 pKa = 10.2GHH14 pKa = 6.6SINSVATKK22 pKa = 10.44CGVQRR27 pKa = 11.84GTIYY31 pKa = 10.81SFGRR35 pKa = 11.84GAGGTQLSTIRR46 pKa = 11.84KK47 pKa = 7.41LAKK50 pKa = 9.58EE51 pKa = 4.07LGMKK55 pKa = 10.04PSEE58 pKa = 4.83LIRR61 pKa = 11.84FMGEE65 pKa = 3.3

Molecular weight:
7.13 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11934

36

940

217.0

24.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.846 ± 0.313

0.997 ± 0.136

6.435 ± 0.276

7.541 ± 0.546

4.349 ± 0.221

7.005 ± 0.304

1.399 ± 0.126

6.268 ± 0.232

6.863 ± 0.514

8.12 ± 0.213

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.288 ± 0.166

5.363 ± 0.218

3.335 ± 0.194

3.855 ± 0.157

4.282 ± 0.253

8.061 ± 0.33

5.807 ± 0.369

6.645 ± 0.291

1.19 ± 0.118

3.352 ± 0.163

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski