Vibrio phage XM1
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7D7JI10|A0A7D7JI10_9CAUD Pesticin domain-containing protein OS=Vibrio phage XM1 OX=2748688 PE=4 SV=1
MM1 pKa = 7.7 ALIDD5 pKa = 3.92 DD6 pKa = 5.11 FKK8 pKa = 11.72 ARR10 pKa = 11.84 FPNLDD15 pKa = 3.17 GSLVDD20 pKa = 4.31 ALVPVYY26 pKa = 10.16 EE27 pKa = 4.5 NNYY30 pKa = 7.53 SCYY33 pKa = 10.52 YY34 pKa = 9.13 GGSYY38 pKa = 11.0 EE39 pKa = 4.04 NDD41 pKa = 3.38 CDD43 pKa = 4.63 KK44 pKa = 11.26 EE45 pKa = 5.35 AILLLIAHH53 pKa = 6.91 LVVTDD58 pKa = 3.95 PSYY61 pKa = 11.68 SGDD64 pKa = 3.56 EE65 pKa = 4.1 SSSRR69 pKa = 11.84 AVASQSVGSVSVSFVAGSTGSDD91 pKa = 2.65 WTNWLNSTRR100 pKa = 11.84 YY101 pKa = 8.7 GQLFLMVTSNNMGPSFAA118 pKa = 5.17
Molecular weight: 12.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.906
IPC2_protein 4.05
IPC_protein 3.973
Toseland 3.757
ProMoST 4.151
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.935
Rodwell 3.795
Grimsley 3.668
Solomon 3.948
Lehninger 3.91
Nozaki 4.101
DTASelect 4.342
Thurlkill 3.834
EMBOSS 3.935
Sillero 4.088
Patrickios 0.362
IPC_peptide 3.948
IPC2_peptide 4.062
IPC2.peptide.svr19 3.978
Protein with the highest isoelectric point:
>tr|A0A7D7EXM6|A0A7D7EXM6_9CAUD PhageMetallopep domain-containing protein OS=Vibrio phage XM1 OX=2748688 PE=4 SV=1
MM1 pKa = 7.31 KK2 pKa = 10.48 KK3 pKa = 9.9 EE4 pKa = 3.92 LRR6 pKa = 11.84 DD7 pKa = 3.99 LITKK11 pKa = 9.91 KK12 pKa = 10.2 GHH14 pKa = 6.6 SINSVATKK22 pKa = 10.44 CGVQRR27 pKa = 11.84 GTIYY31 pKa = 10.81 SFGRR35 pKa = 11.84 GAGGTQLSTIRR46 pKa = 11.84 KK47 pKa = 7.41 LAKK50 pKa = 9.58 EE51 pKa = 4.07 LGMKK55 pKa = 10.04 PSEE58 pKa = 4.83 LIRR61 pKa = 11.84 FMGEE65 pKa = 3.3
Molecular weight: 7.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.178
IPC2_protein 9.516
IPC_protein 9.575
Toseland 10.643
ProMoST 10.101
Dawson 10.716
Bjellqvist 10.292
Wikipedia 10.804
Rodwell 11.301
Grimsley 10.73
Solomon 10.76
Lehninger 10.745
Nozaki 10.613
DTASelect 10.292
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.628
Patrickios 11.082
IPC_peptide 10.774
IPC2_peptide 8.829
IPC2.peptide.svr19 8.562
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
11934
36
940
217.0
24.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.846 ± 0.313
0.997 ± 0.136
6.435 ± 0.276
7.541 ± 0.546
4.349 ± 0.221
7.005 ± 0.304
1.399 ± 0.126
6.268 ± 0.232
6.863 ± 0.514
8.12 ± 0.213
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.288 ± 0.166
5.363 ± 0.218
3.335 ± 0.194
3.855 ± 0.157
4.282 ± 0.253
8.061 ± 0.33
5.807 ± 0.369
6.645 ± 0.291
1.19 ± 0.118
3.352 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here