Rice gall dwarf virus (RGDV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Phytoreovirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q1WNZ7|MCE_RGDV Putative mRNA-capping enzyme P5 OS=Rice gall dwarf virus OX=10986 PE=3 SV=1
MM1 pKa = 7.68AGKK4 pKa = 9.4LQDD7 pKa = 3.37GVAIAKK13 pKa = 9.87IKK15 pKa = 9.0EE16 pKa = 4.39TINLFCEE23 pKa = 4.4YY24 pKa = 11.03SFGDD28 pKa = 3.47LVNNRR33 pKa = 11.84RR34 pKa = 11.84EE35 pKa = 4.06IVGRR39 pKa = 11.84VHH41 pKa = 7.93DD42 pKa = 4.23ARR44 pKa = 11.84KK45 pKa = 9.27NAALAWPDD53 pKa = 4.76LIMNCFLHH61 pKa = 6.5SASHH65 pKa = 5.48YY66 pKa = 10.24GVVKK70 pKa = 10.28FLLDD74 pKa = 3.13IALSTRR80 pKa = 11.84FGDD83 pKa = 3.85FTLLGVSSQNYY94 pKa = 8.26PFYY97 pKa = 10.58DD98 pKa = 3.34LHH100 pKa = 8.66VVMTKK105 pKa = 10.51AFCNLDD111 pKa = 3.63FAKK114 pKa = 10.71DD115 pKa = 3.79EE116 pKa = 4.09YY117 pKa = 12.0LMINDD122 pKa = 4.62SFSSMMSAFLDD133 pKa = 3.75EE134 pKa = 5.04EE135 pKa = 5.08GVHH138 pKa = 6.0SAMSMEE144 pKa = 4.67LGIHH148 pKa = 6.75DD149 pKa = 3.66IEE151 pKa = 4.71DD152 pKa = 3.51RR153 pKa = 11.84FVLRR157 pKa = 11.84TKK159 pKa = 10.08RR160 pKa = 11.84LFYY163 pKa = 10.37IIHH166 pKa = 7.02EE167 pKa = 4.29YY168 pKa = 10.78HH169 pKa = 6.16MSLDD173 pKa = 4.21EE174 pKa = 4.24IEE176 pKa = 4.48PWLEE180 pKa = 3.88KK181 pKa = 11.03LPDD184 pKa = 3.74ASGGTLLNQKK194 pKa = 9.78SKK196 pKa = 10.54EE197 pKa = 3.83QMRR200 pKa = 11.84VIFSNAKK207 pKa = 9.12VRR209 pKa = 11.84IANSINLYY217 pKa = 8.55VTTHH221 pKa = 5.37TNSYY225 pKa = 9.2NEE227 pKa = 4.13YY228 pKa = 8.09VRR230 pKa = 11.84EE231 pKa = 4.0VAEE234 pKa = 4.18YY235 pKa = 10.86VADD238 pKa = 3.96LWNIQTTTNTQGHH251 pKa = 5.69EE252 pKa = 4.12NEE254 pKa = 4.59LAAEE258 pKa = 4.26DD259 pKa = 4.42FGVLASSSQMNGTKK273 pKa = 9.92SEE275 pKa = 4.59LGDD278 pKa = 3.92SVIKK282 pKa = 10.82SDD284 pKa = 3.57GNEE287 pKa = 4.02VKK289 pKa = 10.75LEE291 pKa = 3.8PAMFTRR297 pKa = 11.84NDD299 pKa = 3.85DD300 pKa = 3.9EE301 pKa = 4.87EE302 pKa = 4.27EE303 pKa = 4.07LAGSEE308 pKa = 4.33FTSLLSDD315 pKa = 4.2DD316 pKa = 4.24GRR318 pKa = 11.84MGG320 pKa = 3.35

Molecular weight: 36.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|O56834|P2_RGDV Minor outer capsid protein P2 OS=Rice gall dwarf virus OX=10986 PE=1 SV=1
MM1 pKa = 7.42TSNEE5 pKa = 4.15EE6 pKa = 4.11NPVGVQTLKK15 pKa = 10.78PNNDD19 pKa = 3.25AKK21 pKa = 10.72RR22 pKa = 11.84VFEE25 pKa = 3.9MRR27 pKa = 11.84KK28 pKa = 8.77IPSSNVMRR36 pKa = 11.84VEE38 pKa = 3.78YY39 pKa = 9.74TKK41 pKa = 9.85QSKK44 pKa = 10.06YY45 pKa = 8.15RR46 pKa = 11.84TCVCPSALHH55 pKa = 5.94FAEE58 pKa = 4.9EE59 pKa = 4.62CPSNDD64 pKa = 2.93VLFSVGAHH72 pKa = 5.84RR73 pKa = 11.84NMLCVAKK80 pKa = 10.67AFDD83 pKa = 3.57QTKK86 pKa = 9.21PRR88 pKa = 11.84GMQRR92 pKa = 11.84GNRR95 pKa = 11.84RR96 pKa = 11.84NNVAKK101 pKa = 10.84VMTLNEE107 pKa = 4.95LMPKK111 pKa = 10.21DD112 pKa = 4.4DD113 pKa = 4.23SNDD116 pKa = 3.2RR117 pKa = 11.84KK118 pKa = 10.23KK119 pKa = 11.1AKK121 pKa = 9.03TSKK124 pKa = 10.11DD125 pKa = 3.12RR126 pKa = 11.84KK127 pKa = 9.03VEE129 pKa = 3.74KK130 pKa = 10.12SSRR133 pKa = 11.84GRR135 pKa = 11.84ASSSRR140 pKa = 11.84SRR142 pKa = 11.84GRR144 pKa = 11.84GGFSRR149 pKa = 11.84NGRR152 pKa = 11.84DD153 pKa = 3.23RR154 pKa = 11.84RR155 pKa = 11.84EE156 pKa = 3.69TDD158 pKa = 3.16SQDD161 pKa = 3.41EE162 pKa = 4.23YY163 pKa = 11.87SEE165 pKa = 4.6DD166 pKa = 3.21NSYY169 pKa = 11.2KK170 pKa = 10.29SEE172 pKa = 4.09EE173 pKa = 4.27EE174 pKa = 4.01STEE177 pKa = 4.69DD178 pKa = 2.98EE179 pKa = 4.35TAPLTKK185 pKa = 9.76KK186 pKa = 10.79DD187 pKa = 3.91MLKK190 pKa = 9.75MMSMFQSGAKK200 pKa = 9.26SKK202 pKa = 10.48RR203 pKa = 11.84RR204 pKa = 11.84PRR206 pKa = 3.77

Molecular weight: 23.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

7782

206

1458

648.5

72.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.808 ± 0.306

1.079 ± 0.096

6.181 ± 0.324

5.423 ± 0.392

3.765 ± 0.252

5.217 ± 0.375

1.748 ± 0.163

7.466 ± 0.359

5.795 ± 0.469

8.314 ± 0.404

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.624 ± 0.169

6.412 ± 0.252

3.958 ± 0.317

2.917 ± 0.252

4.87 ± 0.369

9.098 ± 0.275

6.207 ± 0.308

7.183 ± 0.398

0.784 ± 0.097

4.151 ± 0.305
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski