Frigoribacterium sp. Leaf172

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Frigoribacterium; unclassified Frigoribacterium

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2858 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5N8R6|A0A0Q5N8R6_9MICO Uncharacterized protein OS=Frigoribacterium sp. Leaf172 OX=1736285 GN=ASF89_01875 PE=4 SV=1
MM1 pKa = 7.15GAAAAASLALVLTGCSAGGSNSSSTPSDD29 pKa = 3.19TLVVYY34 pKa = 8.99TGQAGDD40 pKa = 3.56YY41 pKa = 9.88QINFNPYY48 pKa = 8.18SPSSIGGIGAIYY60 pKa = 10.15EE61 pKa = 4.3SLFFVTNVNTEE72 pKa = 3.67AYY74 pKa = 8.31EE75 pKa = 3.96PLLGTEE81 pKa = 4.36YY82 pKa = 9.84TWNDD86 pKa = 3.26AGTEE90 pKa = 4.01LAVTLRR96 pKa = 11.84DD97 pKa = 3.53DD98 pKa = 4.01ATWSDD103 pKa = 4.29GEE105 pKa = 4.82PFTADD110 pKa = 2.79DD111 pKa = 4.06VVFTFQMLLDD121 pKa = 4.12TPSLNTSGFDD131 pKa = 3.82GAVTAPDD138 pKa = 3.51DD139 pKa = 3.71THH141 pKa = 8.99VVFTWDD147 pKa = 2.94EE148 pKa = 4.01PAFVAGPTLLGRR160 pKa = 11.84TPIVPEE166 pKa = 4.32HH167 pKa = 6.34LWSGIDD173 pKa = 3.34PTTDD177 pKa = 2.75VMAEE181 pKa = 4.01PVGTGAFTLEE191 pKa = 4.09NFKK194 pKa = 11.11AQAFTLASNPEE205 pKa = 3.98YY206 pKa = 10.09WGGEE210 pKa = 3.96PEE212 pKa = 4.42VKK214 pKa = 9.92KK215 pKa = 11.02VRR217 pKa = 11.84YY218 pKa = 9.31LSLSGNTAGADD229 pKa = 3.23ALAAGTIDD237 pKa = 3.65WQTGPVPDD245 pKa = 4.28MEE247 pKa = 4.51NVSEE251 pKa = 4.45NYY253 pKa = 9.45PGYY256 pKa = 10.56DD257 pKa = 3.44AVTIPQNQVALLTCSSVEE275 pKa = 4.16LGCEE279 pKa = 4.6GPQTDD284 pKa = 3.7PAVRR288 pKa = 11.84QAIYY292 pKa = 10.01HH293 pKa = 6.15ALNRR297 pKa = 11.84DD298 pKa = 3.56QVNSLAFQNTASEE311 pKa = 4.12ISPTFALTSTQEE323 pKa = 4.1DD324 pKa = 4.85LISADD329 pKa = 3.16VAEE332 pKa = 4.98PVAPSAPDD340 pKa = 3.46LDD342 pKa = 4.16KK343 pKa = 11.36SAEE346 pKa = 4.04LLEE349 pKa = 4.53GAGYY353 pKa = 8.36TKK355 pKa = 10.9GGDD358 pKa = 3.16GVYY361 pKa = 10.69AKK363 pKa = 10.68DD364 pKa = 3.91GEE366 pKa = 4.33PLKK369 pKa = 10.59LTVEE373 pKa = 4.65VVTGWTDD380 pKa = 3.58YY381 pKa = 9.72ITAIDD386 pKa = 3.96TMGQQLKK393 pKa = 10.41AAGIEE398 pKa = 4.15LTASQSSWNEE408 pKa = 3.51WTDD411 pKa = 3.27KK412 pKa = 10.66KK413 pKa = 11.42SKK415 pKa = 10.77GNYY418 pKa = 8.5EE419 pKa = 3.96LAIDD423 pKa = 4.46SLGQGPASDD432 pKa = 4.07PFYY435 pKa = 11.08LYY437 pKa = 10.62DD438 pKa = 3.35QYY440 pKa = 11.93FNTAYY445 pKa = 8.97TAEE448 pKa = 4.27VGEE451 pKa = 4.22AAPTNMARR459 pKa = 11.84FSDD462 pKa = 4.21PAVDD466 pKa = 3.65AALAEE471 pKa = 4.4LKK473 pKa = 10.78AVDD476 pKa = 4.32PADD479 pKa = 3.47TAARR483 pKa = 11.84QAQFDD488 pKa = 4.37VIQAAIVEE496 pKa = 4.47DD497 pKa = 3.91MPYY500 pKa = 10.07IPVMTGGTTSEE511 pKa = 4.01FHH513 pKa = 6.61SSKK516 pKa = 9.43FTGWPTMDD524 pKa = 4.96DD525 pKa = 4.17LYY527 pKa = 11.3AFPAIWASPDD537 pKa = 3.04NAQVFKK543 pKa = 10.84NLKK546 pKa = 7.53PTGEE550 pKa = 3.98

Molecular weight: 58.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5N197|A0A0Q5N197_9MICO Epimerase domain-containing protein OS=Frigoribacterium sp. Leaf172 OX=1736285 GN=ASF89_04105 PE=4 SV=1
MM1 pKa = 7.01TRR3 pKa = 11.84RR4 pKa = 11.84GGAALRR10 pKa = 11.84RR11 pKa = 11.84GQLAVSKK18 pKa = 10.65VLAFVSALALLVGLVRR34 pKa = 11.84ILQAHH39 pKa = 5.43VVAGAAFVIIAVALIAVAFALARR62 pKa = 11.84AATRR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 3.56

Molecular weight: 7.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2858

0

2858

913754

38

1957

319.7

33.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.302 ± 0.056

0.425 ± 0.009

6.771 ± 0.051

5.39 ± 0.044

2.975 ± 0.032

9.266 ± 0.045

1.885 ± 0.023

3.95 ± 0.033

1.734 ± 0.034

10.048 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.625 ± 0.017

1.691 ± 0.021

5.398 ± 0.036

2.549 ± 0.028

7.426 ± 0.055

6.123 ± 0.032

6.412 ± 0.058

9.804 ± 0.05

1.374 ± 0.021

1.851 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski