Le Dantec virus
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D3R1W0|A0A0D3R1W0_9RHAB Phosphoprotein OS=Le Dantec virus OX=318848 PE=4 SV=1
MM1 pKa = 7.48 LSSRR5 pKa = 11.84 DD6 pKa = 3.4 RR7 pKa = 11.84 VKK9 pKa = 11.03 AIASKK14 pKa = 11.0 EE15 pKa = 3.92 FFNDD19 pKa = 2.63 IDD21 pKa = 3.78 GKK23 pKa = 9.94 QFEE26 pKa = 4.33 EE27 pKa = 4.23 DD28 pKa = 3.16 QGEE31 pKa = 4.3 EE32 pKa = 4.12 EE33 pKa = 4.69 HH34 pKa = 6.77 VSPVPEE40 pKa = 4.48 IDD42 pKa = 4.18 SDD44 pKa = 4.05 FKK46 pKa = 11.21 KK47 pKa = 10.96 LKK49 pKa = 10.62 GSLDD53 pKa = 3.63 DD54 pKa = 4.29 KK55 pKa = 11.32 EE56 pKa = 4.4 EE57 pKa = 4.15 EE58 pKa = 4.2 EE59 pKa = 4.94 GFDD62 pKa = 4.14 DD63 pKa = 3.47 VTEE66 pKa = 4.27 IVDD69 pKa = 3.91 EE70 pKa = 4.58 NGSDD74 pKa = 3.65 SDD76 pKa = 4.01 SAGDD80 pKa = 3.57 INLMISNSEE89 pKa = 3.95 NSGDD93 pKa = 4.16 DD94 pKa = 3.94 DD95 pKa = 4.36 NPAALVGRR103 pKa = 11.84 HH104 pKa = 5.56 EE105 pKa = 4.74 YY106 pKa = 10.44 IEE108 pKa = 4.3 TDD110 pKa = 3.36 LKK112 pKa = 11.2 HH113 pKa = 6.25 FALSDD118 pKa = 3.55 EE119 pKa = 4.93 LEE121 pKa = 4.29 SHH123 pKa = 7.07 LSSQEE128 pKa = 3.43 EE129 pKa = 4.14 IAAHH133 pKa = 5.93 EE134 pKa = 4.54 SGHH137 pKa = 6.26 RR138 pKa = 11.84 NSSLNISLPKK148 pKa = 10.37 LVLDD152 pKa = 4.9 EE153 pKa = 5.38 IKK155 pKa = 10.52 DD156 pKa = 3.7 QKK158 pKa = 11.17 EE159 pKa = 3.66 LDD161 pKa = 3.97 EE162 pKa = 4.49 TCMGLLHH169 pKa = 7.1 AFAAHH174 pKa = 6.93 LGWSVVPMYY183 pKa = 10.84 SQVSEE188 pKa = 4.11 EE189 pKa = 3.82 KK190 pKa = 10.87 LIINVKK196 pKa = 6.97 QVKK199 pKa = 9.67 RR200 pKa = 11.84 SKK202 pKa = 9.6 QHH204 pKa = 5.28 MSISSTDD211 pKa = 2.81 MDD213 pKa = 3.68 QPVVEE218 pKa = 4.89 NLAKK222 pKa = 10.34 VNRR225 pKa = 11.84 GRR227 pKa = 11.84 SSEE230 pKa = 4.03 RR231 pKa = 11.84 FEE233 pKa = 5.41 GKK235 pKa = 9.97 DD236 pKa = 3.02 KK237 pKa = 11.27 EE238 pKa = 4.28 RR239 pKa = 11.84 FKK241 pKa = 11.48 EE242 pKa = 4.51 FVEE245 pKa = 4.52 KK246 pKa = 10.28 MSSGQIRR253 pKa = 11.84 FIKK256 pKa = 9.97 KK257 pKa = 9.27 KK258 pKa = 9.74 KK259 pKa = 10.09 NGFISLTLATPGVTMEE275 pKa = 4.63 LVKK278 pKa = 10.58 QALDD282 pKa = 3.32 QCVLEE287 pKa = 4.76 KK288 pKa = 11.03 DD289 pKa = 4.15 VIDD292 pKa = 4.19 MILRR296 pKa = 11.84 EE297 pKa = 4.62 SNMLTLVKK305 pKa = 9.13 TLCIYY310 pKa = 10.01 PP311 pKa = 3.83
Molecular weight: 35.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.796
IPC2_protein 4.698
IPC_protein 4.647
Toseland 4.52
ProMoST 4.762
Dawson 4.596
Bjellqvist 4.749
Wikipedia 4.457
Rodwell 4.507
Grimsley 4.431
Solomon 4.596
Lehninger 4.558
Nozaki 4.711
DTASelect 4.863
Thurlkill 4.507
EMBOSS 4.482
Sillero 4.774
Patrickios 4.228
IPC_peptide 4.609
IPC2_peptide 4.762
IPC2.peptide.svr19 4.746
Protein with the highest isoelectric point:
>tr|A0A0D3R1R3|A0A0D3R1R3_9RHAB GDP polyribonucleotidyltransferase OS=Le Dantec virus OX=318848 PE=4 SV=1
MM1 pKa = 7.56 FSRR4 pKa = 11.84 FKK6 pKa = 10.93 KK7 pKa = 10.53 SLSKK11 pKa = 10.55 KK12 pKa = 10.16 GKK14 pKa = 9.89 NDD16 pKa = 3.9 DD17 pKa = 3.65 NQLLYY22 pKa = 10.77 DD23 pKa = 4.34 PPAYY27 pKa = 10.52 DD28 pKa = 3.7 SDD30 pKa = 5.48 DD31 pKa = 3.52 FFAPIKK37 pKa = 10.37 PSAPDD42 pKa = 3.62 LEE44 pKa = 6.12 LEE46 pKa = 4.41 DD47 pKa = 4.31 FRR49 pKa = 11.84 KK50 pKa = 8.53 EE51 pKa = 4.18 TLHH54 pKa = 7.74 VEE56 pKa = 3.78 AEE58 pKa = 4.56 LIIKK62 pKa = 6.98 THH64 pKa = 6.22 AGIKK68 pKa = 9.23 TMEE71 pKa = 4.43 EE72 pKa = 3.76 LLKK75 pKa = 10.7 ILGIWIDD82 pKa = 3.54 QCSGPIRR89 pKa = 11.84 QRR91 pKa = 11.84 HH92 pKa = 5.43 LDD94 pKa = 3.33 LWVYY98 pKa = 10.84 LCLGLHH104 pKa = 5.5 VRR106 pKa = 11.84 RR107 pKa = 11.84 DD108 pKa = 3.82 VGIKK112 pKa = 9.91 SYY114 pKa = 11.01 NVYY117 pKa = 10.16 RR118 pKa = 11.84 AALDD122 pKa = 3.47 SSVVFLHH129 pKa = 7.06 APKK132 pKa = 9.16 TQGKK136 pKa = 8.16 FQFVPCHH143 pKa = 5.4 QFFTQKK149 pKa = 10.91 YY150 pKa = 8.04 KK151 pKa = 11.27 GKK153 pKa = 10.22 DD154 pKa = 3.11 CDD156 pKa = 3.69 VDD158 pKa = 4.32 FTCKK162 pKa = 10.19 FKK164 pKa = 9.64 EE165 pKa = 4.51 TKK167 pKa = 9.15 RR168 pKa = 11.84 TGIPAHH174 pKa = 6.28 TIYY177 pKa = 11.1 NQVLKK182 pKa = 10.96 SGFPPPKK189 pKa = 9.17 PIEE192 pKa = 4.05 VFAGYY197 pKa = 10.61 DD198 pKa = 3.26 VTVSVNKK205 pKa = 10.49 NGEE208 pKa = 3.98 HH209 pKa = 6.86 EE210 pKa = 4.33 IAASATT216 pKa = 3.55
Molecular weight: 24.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.219
IPC2_protein 7.585
IPC_protein 7.527
Toseland 7.307
ProMoST 8.009
Dawson 8.229
Bjellqvist 8.478
Wikipedia 8.156
Rodwell 8.244
Grimsley 7.351
Solomon 8.361
Lehninger 8.375
Nozaki 8.712
DTASelect 8.287
Thurlkill 8.361
EMBOSS 8.463
Sillero 8.653
Patrickios 4.457
IPC_peptide 8.361
IPC2_peptide 7.424
IPC2.peptide.svr19 7.58
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3714
64
2126
619.0
70.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.985 ± 0.778
1.939 ± 0.301
6.327 ± 0.41
6.651 ± 0.739
4.793 ± 0.358
6.193 ± 0.352
2.881 ± 0.286
6.893 ± 0.825
7.27 ± 0.432
9.828 ± 0.663
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.746 ± 0.196
4.604 ± 0.367
4.308 ± 0.258
2.8 ± 0.196
5.197 ± 0.413
7.997 ± 0.367
5.089 ± 0.413
5.52 ± 0.688
1.642 ± 0.217
3.339 ± 0.321
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here