Penicillium stoloniferum virus F
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4G3H2|Q4G3H2_9VIRU Putative dsRNA-dependent RNA polymerase OS=Penicillium stoloniferum virus F OX=296210 PE=4 SV=1
MM1 pKa = 7.92 SFEE4 pKa = 4.24 TSEE7 pKa = 4.11 GMSRR11 pKa = 11.84 PGDD14 pKa = 3.54 NPNKK18 pKa = 10.32 LNAKK22 pKa = 8.69 PRR24 pKa = 11.84 QSARR28 pKa = 11.84 PKK30 pKa = 9.11 TRR32 pKa = 11.84 NSTAQSNQTMRR43 pKa = 11.84 LGWIDD48 pKa = 4.7 PLPQVDD54 pKa = 4.42 TIFPLGLEE62 pKa = 4.5 PNVEE66 pKa = 4.66 SIPAGEE72 pKa = 4.35 VEE74 pKa = 5.41 LDD76 pKa = 3.53 FNLPEE81 pKa = 4.38 TIAKK85 pKa = 9.35 PFADD89 pKa = 3.58 TVTSVGDD96 pKa = 4.28 RR97 pKa = 11.84 IQLVDD102 pKa = 4.32 DD103 pKa = 4.59 DD104 pKa = 5.09 KK105 pKa = 12.05 EE106 pKa = 4.48 NIATSIYY113 pKa = 10.37 GLSFFKK119 pKa = 10.59 AARR122 pKa = 11.84 QLYY125 pKa = 8.14 STMLDD130 pKa = 3.05 HH131 pKa = 7.2 EE132 pKa = 4.67 KK133 pKa = 10.83 AVNQPLKK140 pKa = 10.47 AVYY143 pKa = 10.17 YY144 pKa = 10.5 DD145 pKa = 3.65 EE146 pKa = 4.75 TPIPAHH152 pKa = 5.97 MSGALGIIGHH162 pKa = 6.41 MKK164 pKa = 9.66 TKK166 pKa = 10.67 VGDD169 pKa = 3.93 VLVKK173 pKa = 10.72 DD174 pKa = 3.67 AGVLFKK180 pKa = 10.72 RR181 pKa = 11.84 GTAAGVTKK189 pKa = 10.62 FSEE192 pKa = 3.95 IDD194 pKa = 3.64 NDD196 pKa = 3.92 KK197 pKa = 9.73 TWNLDD202 pKa = 3.23 CSKK205 pKa = 10.66 LVWADD210 pKa = 3.45 HH211 pKa = 6.66 SSLSMIKK218 pKa = 10.15 RR219 pKa = 11.84 LASEE223 pKa = 5.13 KK224 pKa = 10.18 ISQLVKK230 pKa = 9.48 QRR232 pKa = 11.84 YY233 pKa = 8.49 RR234 pKa = 11.84 VTDD237 pKa = 3.71 AQGHH241 pKa = 5.21 VYY243 pKa = 9.78 SVSMPQLTDD252 pKa = 3.15 QALPDD257 pKa = 4.25 YY258 pKa = 9.93 YY259 pKa = 11.35 DD260 pKa = 4.84 SIPDD264 pKa = 3.43 VAPNSDD270 pKa = 3.4 QLRR273 pKa = 11.84 VLTAALQMSLAQFRR287 pKa = 11.84 NDD289 pKa = 3.84 EE290 pKa = 4.34 LPHH293 pKa = 8.28 DD294 pKa = 4.55 EE295 pKa = 5.32 DD296 pKa = 6.08 RR297 pKa = 11.84 SDD299 pKa = 5.4 LLTTLDD305 pKa = 4.31 LLYY308 pKa = 10.93 ADD310 pKa = 4.42 GAYY313 pKa = 9.85 EE314 pKa = 4.09 ISALRR319 pKa = 11.84 DD320 pKa = 3.37 QFEE323 pKa = 4.79 LLMARR328 pKa = 11.84 YY329 pKa = 6.15 TTDD332 pKa = 3.63 FKK334 pKa = 11.18 WRR336 pKa = 11.84 VEE338 pKa = 4.52 SIFKK342 pKa = 10.43 VGPPPAGTTGYY353 pKa = 10.34 GAQTVSSTGNTARR366 pKa = 11.84 WQFPLSDD373 pKa = 3.42 ADD375 pKa = 3.68 INIGYY380 pKa = 9.29 LFSPSKK386 pKa = 10.74 SFSLFPKK393 pKa = 9.24 MVGYY397 pKa = 9.88 SKK399 pKa = 10.51 RR400 pKa = 11.84 ARR402 pKa = 11.84 EE403 pKa = 3.87 DD404 pKa = 3.15 ASASFANSDD413 pKa = 3.29 AKK415 pKa = 10.77 KK416 pKa = 10.16 FYY418 pKa = 11.24 ADD420 pKa = 3.14
Molecular weight: 46.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.169
IPC2_protein 5.194
IPC_protein 5.156
Toseland 5.118
ProMoST 5.245
Dawson 5.194
Bjellqvist 5.321
Wikipedia 5.105
Rodwell 5.092
Grimsley 5.054
Solomon 5.194
Lehninger 5.156
Nozaki 5.321
DTASelect 5.55
Thurlkill 5.143
EMBOSS 5.169
Sillero 5.385
Patrickios 4.279
IPC_peptide 5.194
IPC2_peptide 5.372
IPC2.peptide.svr19 5.382
Protein with the highest isoelectric point:
>tr|Q4G3H2|Q4G3H2_9VIRU Putative dsRNA-dependent RNA polymerase OS=Penicillium stoloniferum virus F OX=296210 PE=4 SV=1
MM1 pKa = 7.28 EE2 pKa = 4.67 TTTPDD7 pKa = 3.87 LPFDD11 pKa = 3.69 LHH13 pKa = 5.51 TRR15 pKa = 11.84 EE16 pKa = 4.38 AYY18 pKa = 10.06 DD19 pKa = 3.12 YY20 pKa = 9.61 ATFHH24 pKa = 6.42 RR25 pKa = 11.84 TLLHH29 pKa = 6.37 KK30 pKa = 10.57 PGLSRR35 pKa = 11.84 IKK37 pKa = 10.05 EE38 pKa = 3.96 DD39 pKa = 2.66 RR40 pKa = 11.84 WVYY43 pKa = 10.45 KK44 pKa = 10.76 YY45 pKa = 10.74 NVEE48 pKa = 4.02 QTRR51 pKa = 11.84 MNTDD55 pKa = 3.09 PFVRR59 pKa = 11.84 KK60 pKa = 9.73 SMKK63 pKa = 10.23 LWDD66 pKa = 3.25 EE67 pKa = 4.16 HH68 pKa = 7.26 AYY70 pKa = 10.66 HH71 pKa = 7.51 DD72 pKa = 4.1 MYY74 pKa = 11.6 GFTKK78 pKa = 10.43 KK79 pKa = 10.42 ARR81 pKa = 11.84 LSNGLDD87 pKa = 3.14 AFQGFAKK94 pKa = 9.82 PQKK97 pKa = 9.4 QRR99 pKa = 11.84 SSMSPEE105 pKa = 3.53 MASCYY110 pKa = 10.16 EE111 pKa = 4.0 KK112 pKa = 10.83 ALEE115 pKa = 4.09 EE116 pKa = 4.07 ARR118 pKa = 11.84 HH119 pKa = 5.35 VFTPHH124 pKa = 5.58 EE125 pKa = 4.15 RR126 pKa = 11.84 LTRR129 pKa = 11.84 LSVPNVCDD137 pKa = 3.48 STNLDD142 pKa = 3.46 SAAGFSFPGKK152 pKa = 9.79 KK153 pKa = 9.67 KK154 pKa = 10.54 SEE156 pKa = 4.24 VVEE159 pKa = 4.14 EE160 pKa = 4.38 AFDD163 pKa = 3.62 VASYY167 pKa = 10.04 IAHH170 pKa = 6.29 FVASDD175 pKa = 3.41 RR176 pKa = 11.84 KK177 pKa = 10.6 VFIPPAKK184 pKa = 9.92 LALRR188 pKa = 11.84 GHH190 pKa = 7.07 LSEE193 pKa = 4.81 IDD195 pKa = 3.61 EE196 pKa = 4.73 LKK198 pKa = 10.07 TRR200 pKa = 11.84 AVWVFPFEE208 pKa = 4.18 ISILEE213 pKa = 4.32 GKK215 pKa = 8.26 WALPYY220 pKa = 11.0 YY221 pKa = 10.45 KK222 pKa = 10.13 FLEE225 pKa = 4.21 QNVPEE230 pKa = 4.13 VHH232 pKa = 6.32 FGEE235 pKa = 5.28 GAMQRR240 pKa = 11.84 LAKK243 pKa = 9.95 TLMTDD248 pKa = 3.39 VASHH252 pKa = 5.55 SEE254 pKa = 4.21 CTEE257 pKa = 3.78 VTLDD261 pKa = 3.13 WSGFDD266 pKa = 3.28 TSVSNWLIDD275 pKa = 3.69 DD276 pKa = 4.65 AFDD279 pKa = 4.2 IMFDD283 pKa = 4.01 SFDD286 pKa = 3.72 EE287 pKa = 4.54 TQVEE291 pKa = 4.08 HH292 pKa = 7.42 DD293 pKa = 4.06 GNFVLGGDD301 pKa = 3.73 HH302 pKa = 6.11 MAKK305 pKa = 10.38 KK306 pKa = 10.21 NEE308 pKa = 4.05 KK309 pKa = 9.49 VKK311 pKa = 10.91 KK312 pKa = 9.78 FLKK315 pKa = 10.04 TYY317 pKa = 9.81 FKK319 pKa = 9.47 KK320 pKa = 9.3 TKK322 pKa = 10.15 IMLPDD327 pKa = 3.89 GSLYY331 pKa = 10.88 KK332 pKa = 10.1 KK333 pKa = 10.32 FHH335 pKa = 7.06 GIPSGSFFTQIIGSIVNYY353 pKa = 10.1 LAVKK357 pKa = 9.34 TLDD360 pKa = 5.25 NYY362 pKa = 10.4 FSWNARR368 pKa = 11.84 RR369 pKa = 11.84 FRR371 pKa = 11.84 VLGDD375 pKa = 3.57 DD376 pKa = 3.86 SSFLIPFGRR385 pKa = 11.84 SKK387 pKa = 11.29 VDD389 pKa = 3.23 GVEE392 pKa = 3.88 ISEE395 pKa = 4.55 KK396 pKa = 10.09 AWEE399 pKa = 4.38 TFGFTLKK406 pKa = 10.51 LKK408 pKa = 10.41 KK409 pKa = 10.41 LRR411 pKa = 11.84 IANKK415 pKa = 8.47 QQDD418 pKa = 4.01 RR419 pKa = 11.84 KK420 pKa = 10.49 FLGYY424 pKa = 9.39 QCNAFRR430 pKa = 11.84 YY431 pKa = 8.17 EE432 pKa = 3.9 RR433 pKa = 11.84 STTEE437 pKa = 3.64 WLSMVLYY444 pKa = 9.55 PEE446 pKa = 4.73 RR447 pKa = 11.84 DD448 pKa = 3.55 VEE450 pKa = 4.37 FLEE453 pKa = 4.68 QSASRR458 pKa = 11.84 VFAFYY463 pKa = 11.32 LLGGCNDD470 pKa = 3.02 VTYY473 pKa = 11.2 CEE475 pKa = 4.74 FFHH478 pKa = 7.67 DD479 pKa = 3.87 YY480 pKa = 10.58 LGRR483 pKa = 11.84 YY484 pKa = 7.79 PYY486 pKa = 9.95 IYY488 pKa = 10.54 GKK490 pKa = 9.55 EE491 pKa = 3.97 LPLTRR496 pKa = 11.84 GLKK499 pKa = 10.21 RR500 pKa = 11.84 LFKK503 pKa = 10.61 FVFRR507 pKa = 11.84 LTIDD511 pKa = 3.1 KK512 pKa = 10.68 LAFPDD517 pKa = 4.38 LSRR520 pKa = 11.84 FDD522 pKa = 3.93 PLKK525 pKa = 11.12 VPFSLSLGDD534 pKa = 3.56 KK535 pKa = 10.15 PFWW538 pKa = 4.63
Molecular weight: 62.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.014
IPC2_protein 7.439
IPC_protein 7.38
Toseland 7.293
ProMoST 7.863
Dawson 8.097
Bjellqvist 8.258
Wikipedia 8.039
Rodwell 8.112
Grimsley 7.38
Solomon 8.185
Lehninger 8.185
Nozaki 8.404
DTASelect 8.141
Thurlkill 8.214
EMBOSS 8.317
Sillero 8.463
Patrickios 4.444
IPC_peptide 8.17
IPC2_peptide 7.234
IPC2.peptide.svr19 7.486
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
958
420
538
479.0
54.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.307 ± 1.152
0.731 ± 0.326
7.516 ± 0.699
5.532 ± 0.825
6.681 ± 1.585
5.428 ± 0.032
2.192 ± 0.505
3.758 ± 0.349
7.203 ± 0.827
9.29 ± 0.161
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.401 ± 0.144
3.236 ± 0.38
5.115 ± 0.554
3.132 ± 0.764
5.219 ± 0.303
7.933 ± 0.738
5.95 ± 0.317
5.846 ± 0.087
1.566 ± 0.248
3.967 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here