Streptococcus satellite phage Javan410
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZPB0|A0A4D5ZPB0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan410 OX=2558687 GN=JavanS410_0006 PE=4 SV=1
MM1 pKa = 7.6 KK2 pKa = 10.4 NLEE5 pKa = 4.17 EE6 pKa = 5.44 KK7 pKa = 10.32 IEE9 pKa = 4.01 PVVIILDD16 pKa = 3.82 EE17 pKa = 4.39 EE18 pKa = 4.81 DD19 pKa = 5.15 DD20 pKa = 4.17 FDD22 pKa = 6.03 FEE24 pKa = 4.36 EE25 pKa = 4.73 TDD27 pKa = 5.58 DD28 pKa = 4.88 EE29 pKa = 4.43 IQAQYY34 pKa = 10.81 EE35 pKa = 4.17 ADD37 pKa = 3.88 CLEE40 pKa = 5.86 DD41 pKa = 3.66 DD42 pKa = 4.18 TQDD45 pKa = 3.43 VINWINEE52 pKa = 4.11 SNTLAEE58 pKa = 4.29 YY59 pKa = 11.21 VSMEE63 pKa = 4.42 FEE65 pKa = 5.29 NMNQDD70 pKa = 3.68 DD71 pKa = 4.68 LGASLEE77 pKa = 4.2 NASQCLDD84 pKa = 4.31 AIVGMLEE91 pKa = 3.76 NGTLVLAGGKK101 pKa = 9.33 HH102 pKa = 5.1 EE103 pKa = 4.14 NN104 pKa = 3.61
Molecular weight: 11.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.027
IPC2_protein 3.376
IPC_protein 3.338
Toseland 3.147
ProMoST 3.516
Dawson 3.338
Bjellqvist 3.49
Wikipedia 3.261
Rodwell 3.185
Grimsley 3.058
Solomon 3.3
Lehninger 3.249
Nozaki 3.465
DTASelect 3.617
Thurlkill 3.21
EMBOSS 3.274
Sillero 3.465
Patrickios 1.697
IPC_peptide 3.287
IPC2_peptide 3.427
IPC2.peptide.svr19 3.638
Protein with the highest isoelectric point:
>tr|A0A4D5ZMG2|A0A4D5ZMG2_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan410 OX=2558687 GN=JavanS410_0010 PE=4 SV=1
MM1 pKa = 7.92 KK2 pKa = 9.25 ITEE5 pKa = 4.15 YY6 pKa = 10.63 KK7 pKa = 9.64 KK8 pKa = 10.76 KK9 pKa = 10.68 NGATVYY15 pKa = 10.31 RR16 pKa = 11.84 SQVYY20 pKa = 10.38 LGIDD24 pKa = 3.31 VVTGKK29 pKa = 10.22 KK30 pKa = 10.34 VKK32 pKa = 9.5 TSVSARR38 pKa = 11.84 TKK40 pKa = 10.94 KK41 pKa = 9.63 EE42 pKa = 3.83 VKK44 pKa = 10.37 LLATQKK50 pKa = 10.89 KK51 pKa = 10.17 YY52 pKa = 11.07 DD53 pKa = 3.74 FKK55 pKa = 11.52 QNGSTIYY62 pKa = 10.47 KK63 pKa = 9.67 SVNIDD68 pKa = 3.32 TFDD71 pKa = 3.56 EE72 pKa = 4.42 LTEE75 pKa = 4.2 LWLEE79 pKa = 4.32 SYY81 pKa = 10.87 KK82 pKa = 10.45 LTVKK86 pKa = 9.78 PQSYY90 pKa = 10.65 KK91 pKa = 9.82 NTISKK96 pKa = 9.52 IDD98 pKa = 3.53 CHH100 pKa = 5.91 IRR102 pKa = 11.84 PYY104 pKa = 9.88 FGHH107 pKa = 6.21 MKK109 pKa = 10.09 LNKK112 pKa = 9.3 INASMIQVFINDD124 pKa = 3.87 LSKK127 pKa = 11.14 KK128 pKa = 9.2 QDD130 pKa = 3.47 SFVMIRR136 pKa = 11.84 SIISRR141 pKa = 11.84 ILQQGVLLNLIPSNPARR158 pKa = 11.84 DD159 pKa = 3.46 IILPRR164 pKa = 11.84 KK165 pKa = 8.3 KK166 pKa = 9.98 HH167 pKa = 5.86 KK168 pKa = 10.71 DD169 pKa = 3.26 NNKK172 pKa = 9.52 VKK174 pKa = 10.68 FIDD177 pKa = 4.47 KK178 pKa = 10.57 EE179 pKa = 4.14 DD180 pKa = 3.45 LKK182 pKa = 11.32 KK183 pKa = 10.83 FLDD186 pKa = 4.5 HH187 pKa = 7.13 IEE189 pKa = 4.09 KK190 pKa = 10.39 KK191 pKa = 10.56 SYY193 pKa = 10.14 GRR195 pKa = 11.84 YY196 pKa = 9.64 GLYY199 pKa = 10.09 FDD201 pKa = 4.29 YY202 pKa = 11.22 VLYY205 pKa = 11.02 NLLLATGLRR214 pKa = 11.84 VGEE217 pKa = 4.2 ACALEE222 pKa = 4.28 WSDD225 pKa = 4.33 IDD227 pKa = 4.56 LEE229 pKa = 4.33 NGIITVTKK237 pKa = 9.74 TYY239 pKa = 10.89 NKK241 pKa = 9.33 SLKK244 pKa = 10.16 IISTTKK250 pKa = 9.0 TKK252 pKa = 10.6 AGNRR256 pKa = 11.84 IISIGKK262 pKa = 8.46 DD263 pKa = 3.1 TVNLMKK269 pKa = 10.41 LYY271 pKa = 10.44 KK272 pKa = 10.15 ARR274 pKa = 11.84 QRR276 pKa = 11.84 QLFSEE281 pKa = 4.77 LGSNSPRR288 pKa = 11.84 IVFSTPTKK296 pKa = 10.32 EE297 pKa = 4.24 YY298 pKa = 9.99 FDD300 pKa = 3.65 NATRR304 pKa = 11.84 QCALDD309 pKa = 3.98 TMCGQAGVPRR319 pKa = 11.84 FTFHH323 pKa = 7.91 AFRR326 pKa = 11.84 HH327 pKa = 4.69 THH329 pKa = 6.92 ASLLLNAGISYY340 pKa = 10.68 KK341 pKa = 10.23 EE342 pKa = 3.61 LQYY345 pKa = 11.51 RR346 pKa = 11.84 LGHH349 pKa = 6.41 ANISMTLDD357 pKa = 3.31 IYY359 pKa = 11.6 SHH361 pKa = 6.83 LSKK364 pKa = 10.92 DD365 pKa = 3.51 KK366 pKa = 10.41 EE367 pKa = 4.26 KK368 pKa = 10.88 EE369 pKa = 3.87 AVSYY373 pKa = 10.09 FEE375 pKa = 4.84 KK376 pKa = 10.98 AMNGLL381 pKa = 3.71
Molecular weight: 43.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.035
IPC2_protein 9.165
IPC_protein 9.033
Toseland 9.853
ProMoST 9.516
Dawson 10.058
Bjellqvist 9.721
Wikipedia 10.218
Rodwell 10.584
Grimsley 10.131
Solomon 10.072
Lehninger 10.043
Nozaki 9.853
DTASelect 9.706
Thurlkill 9.911
EMBOSS 10.262
Sillero 9.97
Patrickios 9.897
IPC_peptide 10.072
IPC2_peptide 8.214
IPC2.peptide.svr19 8.107
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
23
0
23
3976
44
508
172.9
19.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.98 ± 0.654
0.704 ± 0.158
6.212 ± 0.365
7.797 ± 0.619
4.276 ± 0.485
4.225 ± 0.343
1.258 ± 0.164
7.394 ± 0.508
10.262 ± 0.519
9.18 ± 0.679
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.817 ± 0.363
6.514 ± 0.418
3.018 ± 0.337
3.747 ± 0.367
2.968 ± 0.301
7.394 ± 0.86
6.212 ± 0.456
5.609 ± 0.288
0.88 ± 0.156
4.552 ± 0.346
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here