Pseudomonas phage MR6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Krylovirinae; unclassified Krylovirinae

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3TCX2|A0A6M3TCX2_9CAUD Uncharacterized protein OS=Pseudomonas phage MR6 OX=2711173 GN=PssvBMR6_gp08 PE=4 SV=1
MM1 pKa = 7.34TGSSVGTGGAAWVRR15 pKa = 11.84SGAFGASAGGCEE27 pKa = 4.18NCDD30 pKa = 3.84SIGLTGCAGATTGAVAGAWGCDD52 pKa = 3.27TGPP55 pKa = 3.62

Molecular weight: 4.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3TD05|A0A6M3TD05_9CAUD Putative tegument protein OS=Pseudomonas phage MR6 OX=2711173 GN=PssvBMR6_gp26 PE=4 SV=1
MM1 pKa = 7.7INRR4 pKa = 11.84GYY6 pKa = 10.7RR7 pKa = 11.84RR8 pKa = 11.84LHH10 pKa = 6.19WGRR13 pKa = 11.84CLGGTTGKK21 pKa = 8.23TCWQVLDD28 pKa = 3.86VLRR31 pKa = 11.84HH32 pKa = 4.98GLSS35 pKa = 3.14

Molecular weight: 4.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

13404

31

1012

227.2

24.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.944 ± 0.461

1.343 ± 0.22

5.603 ± 0.199

5.26 ± 0.332

3.051 ± 0.166

7.953 ± 0.334

2.007 ± 0.175

4.133 ± 0.204

4.387 ± 0.25

8.915 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.962 ± 0.147

3.924 ± 0.302

4.312 ± 0.278

4.678 ± 0.296

6.17 ± 0.296

6.103 ± 0.29

5.953 ± 0.353

7.505 ± 0.386

1.514 ± 0.135

3.283 ± 0.204
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski