Amino acid dipepetide frequency for Enterobacteria phage SP6 (Bacteriophage SP6)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.896AlaAla: 8.896 ± 1.439
0.754AlaCys: 0.754 ± 0.247
5.202AlaAsp: 5.202 ± 0.67
6.408AlaGlu: 6.408 ± 0.912
2.789AlaPhe: 2.789 ± 0.651
7.312AlaGly: 7.312 ± 1.037
1.432AlaHis: 1.432 ± 0.312
5.805AlaIle: 5.805 ± 0.556
4.9AlaLys: 4.9 ± 0.7
6.785AlaLeu: 6.785 ± 0.735
3.769AlaMet: 3.769 ± 0.733
3.242AlaAsn: 3.242 ± 0.506
3.694AlaPro: 3.694 ± 0.669
4.825AlaGln: 4.825 ± 0.877
5.503AlaArg: 5.503 ± 0.765
5.352AlaSer: 5.352 ± 0.746
4.297AlaThr: 4.297 ± 0.578
7.162AlaVal: 7.162 ± 0.781
1.282AlaTrp: 1.282 ± 0.329
2.865AlaTyr: 2.865 ± 0.422
0.0AlaXaa: 0.0 ± 0.0
Cys
0.377CysAla: 0.377 ± 0.143
0.151CysCys: 0.151 ± 0.113
0.528CysAsp: 0.528 ± 0.248
0.452CysGlu: 0.452 ± 0.196
0.452CysPhe: 0.452 ± 0.181
0.829CysGly: 0.829 ± 0.351
0.452CysHis: 0.452 ± 0.218
0.603CysIle: 0.603 ± 0.19
0.603CysLys: 0.603 ± 0.218
0.905CysLeu: 0.905 ± 0.209
0.452CysMet: 0.452 ± 0.219
0.377CysAsn: 0.377 ± 0.159
0.452CysPro: 0.452 ± 0.185
0.226CysGln: 0.226 ± 0.126
0.678CysArg: 0.678 ± 0.221
0.452CysSer: 0.452 ± 0.186
0.075CysThr: 0.075 ± 0.065
0.754CysVal: 0.754 ± 0.236
0.075CysTrp: 0.075 ± 0.087
0.603CysTyr: 0.603 ± 0.212
0.0CysXaa: 0.0 ± 0.0
Asp
6.031AspAla: 6.031 ± 0.733
0.678AspCys: 0.678 ± 0.349
4.222AspAsp: 4.222 ± 0.698
4.523AspGlu: 4.523 ± 0.626
1.734AspPhe: 1.734 ± 0.312
4.975AspGly: 4.975 ± 0.769
0.98AspHis: 0.98 ± 0.267
3.769AspIle: 3.769 ± 0.5
4.071AspLys: 4.071 ± 0.536
5.88AspLeu: 5.88 ± 0.727
1.583AspMet: 1.583 ± 0.308
2.488AspAsn: 2.488 ± 0.41
2.262AspPro: 2.262 ± 0.385
1.206AspGln: 1.206 ± 0.339
3.015AspArg: 3.015 ± 0.324
4.222AspSer: 4.222 ± 0.443
3.242AspThr: 3.242 ± 0.489
4.372AspVal: 4.372 ± 0.545
0.98AspTrp: 0.98 ± 0.299
2.035AspTyr: 2.035 ± 0.368
0.0AspXaa: 0.0 ± 0.0
Glu
9.046GluAla: 9.046 ± 1.186
0.754GluCys: 0.754 ± 0.28
6.031GluAsp: 6.031 ± 0.686
6.257GluGlu: 6.257 ± 0.851
2.714GluPhe: 2.714 ± 0.402
6.031GluGly: 6.031 ± 0.66
1.583GluHis: 1.583 ± 0.391
2.789GluIle: 2.789 ± 0.371
4.222GluLys: 4.222 ± 0.506
5.805GluLeu: 5.805 ± 0.741
2.563GluMet: 2.563 ± 0.522
1.809GluAsn: 1.809 ± 0.377
1.809GluPro: 1.809 ± 0.394
3.015GluGln: 3.015 ± 0.411
3.392GluArg: 3.392 ± 0.547
3.242GluSer: 3.242 ± 0.384
3.015GluThr: 3.015 ± 0.47
5.503GluVal: 5.503 ± 0.682
1.357GluTrp: 1.357 ± 0.331
1.809GluTyr: 1.809 ± 0.299
0.0GluXaa: 0.0 ± 0.0
Phe
2.94PheAla: 2.94 ± 0.581
0.151PheCys: 0.151 ± 0.107
3.694PheAsp: 3.694 ± 0.513
3.015PheGlu: 3.015 ± 0.452
1.055PhePhe: 1.055 ± 0.307
2.412PheGly: 2.412 ± 0.6
0.603PheHis: 0.603 ± 0.217
2.337PheIle: 2.337 ± 0.291
2.563PheLys: 2.563 ± 0.469
3.242PheLeu: 3.242 ± 0.445
1.357PheMet: 1.357 ± 0.314
2.035PheAsn: 2.035 ± 0.391
1.055PhePro: 1.055 ± 0.236
1.131PheGln: 1.131 ± 0.303
2.111PheArg: 2.111 ± 0.368
2.111PheSer: 2.111 ± 0.428
1.809PheThr: 1.809 ± 0.312
1.357PheVal: 1.357 ± 0.3
0.302PheTrp: 0.302 ± 0.158
0.829PheTyr: 0.829 ± 0.27
0.0PheXaa: 0.0 ± 0.0
Gly
6.785GlyAla: 6.785 ± 0.617
0.754GlyCys: 0.754 ± 0.252
5.503GlyAsp: 5.503 ± 0.639
5.428GlyGlu: 5.428 ± 0.543
3.619GlyPhe: 3.619 ± 0.798
6.106GlyGly: 6.106 ± 0.865
1.96GlyHis: 1.96 ± 0.413
4.9GlyIle: 4.9 ± 0.686
6.483GlyLys: 6.483 ± 0.865
4.975GlyLeu: 4.975 ± 0.782
2.111GlyMet: 2.111 ± 0.356
3.242GlyAsn: 3.242 ± 0.566
0.377GlyPro: 0.377 ± 0.16
3.392GlyGln: 3.392 ± 0.461
4.448GlyArg: 4.448 ± 0.487
5.428GlySer: 5.428 ± 0.789
4.599GlyThr: 4.599 ± 0.648
5.956GlyVal: 5.956 ± 0.757
1.357GlyTrp: 1.357 ± 0.334
2.714GlyTyr: 2.714 ± 0.426
0.0GlyXaa: 0.0 ± 0.0
His
1.055HisAla: 1.055 ± 0.302
0.226HisCys: 0.226 ± 0.147
1.432HisAsp: 1.432 ± 0.241
1.658HisGlu: 1.658 ± 0.415
0.829HisPhe: 0.829 ± 0.21
1.885HisGly: 1.885 ± 0.523
0.603HisHis: 0.603 ± 0.234
1.508HisIle: 1.508 ± 0.458
1.131HisLys: 1.131 ± 0.322
2.488HisLeu: 2.488 ± 0.451
0.754HisMet: 0.754 ± 0.359
0.905HisAsn: 0.905 ± 0.236
0.528HisPro: 0.528 ± 0.212
0.905HisGln: 0.905 ± 0.283
1.055HisArg: 1.055 ± 0.386
0.754HisSer: 0.754 ± 0.284
1.432HisThr: 1.432 ± 0.374
1.432HisVal: 1.432 ± 0.315
0.151HisTrp: 0.151 ± 0.114
0.98HisTyr: 0.98 ± 0.214
0.0HisXaa: 0.0 ± 0.0
Ile
4.9IleAla: 4.9 ± 0.471
0.226IleCys: 0.226 ± 0.134
4.071IleAsp: 4.071 ± 0.567
3.769IleGlu: 3.769 ± 0.512
1.809IlePhe: 1.809 ± 0.456
5.352IleGly: 5.352 ± 0.83
1.282IleHis: 1.282 ± 0.319
3.769IleIle: 3.769 ± 0.716
4.297IleLys: 4.297 ± 0.64
3.468IleLeu: 3.468 ± 0.436
1.658IleMet: 1.658 ± 0.312
2.639IleAsn: 2.639 ± 0.338
1.96IlePro: 1.96 ± 0.398
2.035IleGln: 2.035 ± 0.328
3.543IleArg: 3.543 ± 0.397
2.563IleSer: 2.563 ± 0.49
3.015IleThr: 3.015 ± 0.485
3.995IleVal: 3.995 ± 0.548
0.452IleTrp: 0.452 ± 0.155
1.96IleTyr: 1.96 ± 0.409
0.0IleXaa: 0.0 ± 0.0
Lys
7.011LysAla: 7.011 ± 0.917
0.603LysCys: 0.603 ± 0.205
3.166LysAsp: 3.166 ± 0.584
4.975LysGlu: 4.975 ± 0.721
1.885LysPhe: 1.885 ± 0.296
5.277LysGly: 5.277 ± 0.58
1.885LysHis: 1.885 ± 0.49
2.488LysIle: 2.488 ± 0.344
3.92LysLys: 3.92 ± 0.694
4.825LysLeu: 4.825 ± 0.695
0.905LysMet: 0.905 ± 0.241
1.885LysAsn: 1.885 ± 0.444
3.543LysPro: 3.543 ± 0.551
2.337LysGln: 2.337 ± 0.324
3.845LysArg: 3.845 ± 0.524
4.523LysSer: 4.523 ± 0.472
2.186LysThr: 2.186 ± 0.299
4.222LysVal: 4.222 ± 0.463
0.829LysTrp: 0.829 ± 0.228
2.337LysTyr: 2.337 ± 0.434
0.0LysXaa: 0.0 ± 0.0
Leu
6.332LeuAla: 6.332 ± 1.013
0.829LeuCys: 0.829 ± 0.252
5.202LeuAsp: 5.202 ± 0.579
5.805LeuGlu: 5.805 ± 0.58
2.186LeuPhe: 2.186 ± 0.432
5.654LeuGly: 5.654 ± 0.792
1.206LeuHis: 1.206 ± 0.338
3.995LeuIle: 3.995 ± 0.625
5.051LeuLys: 5.051 ± 0.462
5.051LeuLeu: 5.051 ± 0.592
2.714LeuMet: 2.714 ± 0.547
3.317LeuAsn: 3.317 ± 0.435
3.242LeuPro: 3.242 ± 0.468
3.543LeuGln: 3.543 ± 0.652
4.523LeuArg: 4.523 ± 0.623
5.126LeuSer: 5.126 ± 0.781
4.975LeuThr: 4.975 ± 0.574
5.126LeuVal: 5.126 ± 0.584
1.055LeuTrp: 1.055 ± 0.267
2.412LeuTyr: 2.412 ± 0.48
0.0LeuXaa: 0.0 ± 0.0
Met
3.845MetAla: 3.845 ± 0.43
0.302MetCys: 0.302 ± 0.157
1.357MetAsp: 1.357 ± 0.315
2.035MetGlu: 2.035 ± 0.406
0.829MetPhe: 0.829 ± 0.24
2.412MetGly: 2.412 ± 0.68
0.678MetHis: 0.678 ± 0.3
1.357MetIle: 1.357 ± 0.385
2.035MetLys: 2.035 ± 0.364
3.015MetLeu: 3.015 ± 0.559
0.98MetMet: 0.98 ± 0.275
0.905MetAsn: 0.905 ± 0.271
1.206MetPro: 1.206 ± 0.218
1.508MetGln: 1.508 ± 0.442
1.734MetArg: 1.734 ± 0.481
2.714MetSer: 2.714 ± 0.387
1.96MetThr: 1.96 ± 0.419
1.809MetVal: 1.809 ± 0.409
0.377MetTrp: 0.377 ± 0.137
0.98MetTyr: 0.98 ± 0.271
0.0MetXaa: 0.0 ± 0.0
Asn
3.015AsnAla: 3.015 ± 0.443
0.377AsnCys: 0.377 ± 0.15
1.96AsnAsp: 1.96 ± 0.395
1.96AsnGlu: 1.96 ± 0.543
1.583AsnPhe: 1.583 ± 0.292
3.694AsnGly: 3.694 ± 0.631
1.131AsnHis: 1.131 ± 0.301
2.111AsnIle: 2.111 ± 0.57
2.714AsnLys: 2.714 ± 0.519
3.468AsnLeu: 3.468 ± 0.345
1.131AsnMet: 1.131 ± 0.229
1.96AsnAsn: 1.96 ± 0.323
1.96AsnPro: 1.96 ± 0.434
1.809AsnGln: 1.809 ± 0.541
3.166AsnArg: 3.166 ± 0.422
1.583AsnSer: 1.583 ± 0.371
2.111AsnThr: 2.111 ± 0.424
2.639AsnVal: 2.639 ± 0.398
0.452AsnTrp: 0.452 ± 0.154
1.357AsnTyr: 1.357 ± 0.346
0.0AsnXaa: 0.0 ± 0.0
Pro
3.392ProAla: 3.392 ± 0.433
0.302ProCys: 0.302 ± 0.116
2.789ProAsp: 2.789 ± 0.609
3.242ProGlu: 3.242 ± 0.514
1.206ProPhe: 1.206 ± 0.299
2.186ProGly: 2.186 ± 0.45
0.829ProHis: 0.829 ± 0.323
1.055ProIle: 1.055 ± 0.278
1.809ProLys: 1.809 ± 0.349
2.488ProLeu: 2.488 ± 0.352
1.282ProMet: 1.282 ± 0.238
1.432ProAsn: 1.432 ± 0.381
0.754ProPro: 0.754 ± 0.149
1.583ProGln: 1.583 ± 0.357
1.432ProArg: 1.432 ± 0.302
1.734ProSer: 1.734 ± 0.332
2.262ProThr: 2.262 ± 0.4
3.166ProVal: 3.166 ± 0.497
0.829ProTrp: 0.829 ± 0.247
1.734ProTyr: 1.734 ± 0.382
0.0ProXaa: 0.0 ± 0.0
Gln
4.071GlnAla: 4.071 ± 0.448
0.377GlnCys: 0.377 ± 0.154
1.885GlnAsp: 1.885 ± 0.436
2.563GlnGlu: 2.563 ± 0.43
2.035GlnPhe: 2.035 ± 0.383
2.337GlnGly: 2.337 ± 0.485
0.829GlnHis: 0.829 ± 0.225
2.488GlnIle: 2.488 ± 0.408
1.96GlnLys: 1.96 ± 0.422
3.92GlnLeu: 3.92 ± 0.655
1.583GlnMet: 1.583 ± 0.282
1.734GlnAsn: 1.734 ± 0.383
1.206GlnPro: 1.206 ± 0.278
2.412GlnGln: 2.412 ± 0.481
1.885GlnArg: 1.885 ± 0.464
2.337GlnSer: 2.337 ± 0.359
2.412GlnThr: 2.412 ± 0.405
2.865GlnVal: 2.865 ± 0.377
0.829GlnTrp: 0.829 ± 0.311
1.734GlnTyr: 1.734 ± 0.369
0.0GlnXaa: 0.0 ± 0.0
Arg
4.975ArgAla: 4.975 ± 0.662
0.829ArgCys: 0.829 ± 0.398
3.468ArgAsp: 3.468 ± 0.458
4.674ArgGlu: 4.674 ± 0.714
1.885ArgPhe: 1.885 ± 0.273
4.448ArgGly: 4.448 ± 0.631
1.206ArgHis: 1.206 ± 0.259
3.543ArgIle: 3.543 ± 0.619
2.639ArgLys: 2.639 ± 0.388
4.448ArgLeu: 4.448 ± 0.566
2.488ArgMet: 2.488 ± 0.32
2.412ArgAsn: 2.412 ± 0.445
1.809ArgPro: 1.809 ± 0.354
2.488ArgGln: 2.488 ± 0.416
3.091ArgArg: 3.091 ± 0.518
2.563ArgSer: 2.563 ± 0.409
2.337ArgThr: 2.337 ± 0.481
3.392ArgVal: 3.392 ± 0.451
0.905ArgTrp: 0.905 ± 0.224
2.111ArgTyr: 2.111 ± 0.44
0.0ArgXaa: 0.0 ± 0.0
Ser
5.051SerAla: 5.051 ± 0.822
0.528SerCys: 0.528 ± 0.19
3.619SerAsp: 3.619 ± 0.432
3.995SerGlu: 3.995 ± 0.596
2.412SerPhe: 2.412 ± 0.386
5.654SerGly: 5.654 ± 0.718
1.282SerHis: 1.282 ± 0.257
3.619SerIle: 3.619 ± 0.36
3.015SerLys: 3.015 ± 0.463
4.222SerLeu: 4.222 ± 0.574
1.885SerMet: 1.885 ± 0.428
2.035SerAsn: 2.035 ± 0.352
1.432SerPro: 1.432 ± 0.327
2.639SerGln: 2.639 ± 0.429
3.242SerArg: 3.242 ± 0.491
2.186SerSer: 2.186 ± 0.441
2.865SerThr: 2.865 ± 0.532
3.845SerVal: 3.845 ± 0.597
0.98SerTrp: 0.98 ± 0.225
1.96SerTyr: 1.96 ± 0.382
0.0SerXaa: 0.0 ± 0.0
Thr
3.694ThrAla: 3.694 ± 0.616
0.829ThrCys: 0.829 ± 0.245
2.111ThrAsp: 2.111 ± 0.448
4.222ThrGlu: 4.222 ± 0.477
2.789ThrPhe: 2.789 ± 0.455
4.523ThrGly: 4.523 ± 0.539
1.583ThrHis: 1.583 ± 0.36
3.543ThrIle: 3.543 ± 0.418
3.619ThrLys: 3.619 ± 0.395
4.448ThrLeu: 4.448 ± 0.742
0.98ThrMet: 0.98 ± 0.205
2.262ThrAsn: 2.262 ± 0.423
2.111ThrPro: 2.111 ± 0.33
2.186ThrGln: 2.186 ± 0.425
2.94ThrArg: 2.94 ± 0.522
2.337ThrSer: 2.337 ± 0.365
2.488ThrThr: 2.488 ± 0.43
3.242ThrVal: 3.242 ± 0.474
0.754ThrTrp: 0.754 ± 0.208
1.734ThrTyr: 1.734 ± 0.359
0.0ThrXaa: 0.0 ± 0.0
Val
6.709ValAla: 6.709 ± 0.78
0.377ValCys: 0.377 ± 0.168
3.317ValAsp: 3.317 ± 0.476
4.749ValGlu: 4.749 ± 0.638
2.262ValPhe: 2.262 ± 0.472
5.051ValGly: 5.051 ± 0.693
1.282ValHis: 1.282 ± 0.384
4.222ValIle: 4.222 ± 0.647
4.825ValLys: 4.825 ± 0.582
4.222ValLeu: 4.222 ± 0.692
2.337ValMet: 2.337 ± 0.395
2.94ValAsn: 2.94 ± 0.351
3.769ValPro: 3.769 ± 0.527
2.186ValGln: 2.186 ± 0.432
4.071ValArg: 4.071 ± 0.616
4.146ValSer: 4.146 ± 0.511
4.599ValThr: 4.599 ± 0.666
4.071ValVal: 4.071 ± 0.473
0.678ValTrp: 0.678 ± 0.231
1.658ValTyr: 1.658 ± 0.366
0.0ValXaa: 0.0 ± 0.0
Trp
1.282TrpAla: 1.282 ± 0.248
0.226TrpCys: 0.226 ± 0.108
1.055TrpAsp: 1.055 ± 0.265
1.206TrpGlu: 1.206 ± 0.271
0.528TrpPhe: 0.528 ± 0.228
0.905TrpGly: 0.905 ± 0.229
0.226TrpHis: 0.226 ± 0.133
0.528TrpIle: 0.528 ± 0.205
0.905TrpLys: 0.905 ± 0.302
0.754TrpLeu: 0.754 ± 0.195
0.528TrpMet: 0.528 ± 0.174
0.452TrpAsn: 0.452 ± 0.173
1.131TrpPro: 1.131 ± 0.28
0.754TrpGln: 0.754 ± 0.225
0.754TrpArg: 0.754 ± 0.237
1.055TrpSer: 1.055 ± 0.371
0.452TrpThr: 0.452 ± 0.215
1.055TrpVal: 1.055 ± 0.291
0.226TrpTrp: 0.226 ± 0.111
0.302TrpTyr: 0.302 ± 0.159
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.865TyrAla: 2.865 ± 0.346
0.226TyrCys: 0.226 ± 0.113
1.658TyrAsp: 1.658 ± 0.332
1.809TyrGlu: 1.809 ± 0.361
1.508TyrPhe: 1.508 ± 0.373
3.166TyrGly: 3.166 ± 0.437
0.678TyrHis: 0.678 ± 0.231
2.337TyrIle: 2.337 ± 0.368
1.885TyrLys: 1.885 ± 0.361
2.94TyrLeu: 2.94 ± 0.374
0.905TyrMet: 0.905 ± 0.272
2.111TyrAsn: 2.111 ± 0.389
1.206TyrPro: 1.206 ± 0.354
1.282TyrGln: 1.282 ± 0.26
1.282TyrArg: 1.282 ± 0.353
2.035TyrSer: 2.035 ± 0.486
2.262TyrThr: 2.262 ± 0.304
1.508TyrVal: 1.508 ± 0.296
0.452TyrTrp: 0.452 ± 0.224
0.829TyrTyr: 0.829 ± 0.236
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (13266 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski