Phytophthora parasitica P1569
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 26477 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V9F7K1|V9F7K1_PHYPR Uncharacterized protein OS=Phytophthora parasitica P1569 OX=1317065 GN=F443_08917 PE=4 SV=1
MM1 pKa = 7.07 QVFGVTALVFLALQGSVQAVQNAITCTTTADD32 pKa = 4.08 CAWGEE37 pKa = 4.35 TCVAGDD43 pKa = 4.38 ADD45 pKa = 4.21 TSVQACVAPTVCGGSSMGNCPSDD68 pKa = 3.53 DD69 pKa = 3.83 TGKK72 pKa = 10.26 LACLWRR78 pKa = 11.84 PFNDD82 pKa = 3.49 CSEE85 pKa = 4.19 GCAILNGNKK94 pKa = 10.11 GIYY97 pKa = 9.36 KK98 pKa = 9.7 CVSITRR104 pKa = 11.84 CDD106 pKa = 3.2 AYY108 pKa = 11.34 YY109 pKa = 10.86 GGSKK113 pKa = 10.56 CSDD116 pKa = 3.33 GCSVNGVRR124 pKa = 11.84 CNGQGSCNMMSSNADD139 pKa = 3.18 GTPVFGCTCNNGYY152 pKa = 10.29 SGEE155 pKa = 4.16 KK156 pKa = 10.27 CEE158 pKa = 4.58 NAPGLTNTSSTDD170 pKa = 3.15 TDD172 pKa = 4.45 DD173 pKa = 5.33 EE174 pKa = 5.0 DD175 pKa = 4.87 SFWGALDD182 pKa = 4.37 GGSDD186 pKa = 4.78 DD187 pKa = 5.26 DD188 pKa = 4.56 ASKK191 pKa = 11.23 NGSDD195 pKa = 5.13 DD196 pKa = 4.78 ANADD200 pKa = 3.74 DD201 pKa = 5.94 SGTSDD206 pKa = 5.21 DD207 pKa = 4.56 KK208 pKa = 11.59 STDD211 pKa = 3.3 ASDD214 pKa = 5.54 DD215 pKa = 3.73 SATDD219 pKa = 3.38 EE220 pKa = 4.69 SSTDD224 pKa = 3.33 TSDD227 pKa = 5.54 DD228 pKa = 3.51 SSTDD232 pKa = 3.34 ADD234 pKa = 3.91 QSTTDD239 pKa = 4.91 ASDD242 pKa = 3.3 EE243 pKa = 4.63 SNADD247 pKa = 3.72 DD248 pKa = 4.71 NAEE251 pKa = 4.43 DD252 pKa = 4.61 DD253 pKa = 4.38 SASSSSSASSSGEE266 pKa = 3.78 LANSEE271 pKa = 4.76 SVSSTSRR278 pKa = 11.84 SGIRR282 pKa = 11.84 PGVLILVLVMTAFFLVGTVLLVAYY306 pKa = 9.47 SRR308 pKa = 11.84 RR309 pKa = 11.84 RR310 pKa = 11.84 KK311 pKa = 7.81 QQEE314 pKa = 3.7 EE315 pKa = 4.23 EE316 pKa = 4.47 EE317 pKa = 4.48 YY318 pKa = 11.43 ANALASTQGVGAVGARR334 pKa = 11.84 DD335 pKa = 3.4 LAAGHH340 pKa = 6.21 TGRR343 pKa = 11.84 TPRR346 pKa = 11.84 SNIARR351 pKa = 11.84 MM352 pKa = 3.51
Molecular weight: 35.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.77
IPC_protein 3.821
Toseland 3.579
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.63
Grimsley 3.478
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.215
Thurlkill 3.643
EMBOSS 3.783
Sillero 3.935
Patrickios 0.858
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.81
Protein with the highest isoelectric point:
>tr|V9FPT3|V9FPT3_PHYPR Uncharacterized protein (Fragment) OS=Phytophthora parasitica P1569 OX=1317065 GN=F443_04150 PE=4 SV=1
MM1 pKa = 7.22 SRR3 pKa = 11.84 SPGRR7 pKa = 11.84 SRR9 pKa = 11.84 HH10 pKa = 5.3 GSGAAPVLARR20 pKa = 11.84 SRR22 pKa = 11.84 RR23 pKa = 11.84 KK24 pKa = 10.31 LGTAHH29 pKa = 7.34 AMLAHH34 pKa = 6.89 AKK36 pKa = 8.69 TRR38 pKa = 3.52
Molecular weight: 4.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.468
IPC2_protein 11.023
IPC_protein 12.618
Toseland 12.778
ProMoST 13.29
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.398
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.149
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.128
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
22968
3509
26477
10979262
30
11994
414.7
46.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.605 ± 0.015
1.655 ± 0.007
5.669 ± 0.012
6.675 ± 0.018
3.749 ± 0.01
5.864 ± 0.017
2.34 ± 0.007
4.264 ± 0.01
5.21 ± 0.017
9.4 ± 0.02
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.458 ± 0.007
3.671 ± 0.009
4.671 ± 0.012
4.261 ± 0.012
6.324 ± 0.018
8.368 ± 0.019
6.007 ± 0.014
7.001 ± 0.012
1.207 ± 0.005
2.599 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here