Listeria phage vB_LmoS_188

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5D142|A0A0B5D142_9CAUD Uncharacterized protein OS=Listeria phage vB_LmoS_188 OX=1591072 GN=SE24_054 PE=4 SV=1
MM1 pKa = 7.5ILYY4 pKa = 10.08GVVTYY9 pKa = 10.98DD10 pKa = 4.66EE11 pKa = 4.38ATEE14 pKa = 4.13WTTDD18 pKa = 3.33LLTAKK23 pKa = 10.05KK24 pKa = 9.11WVEE27 pKa = 3.45NAKK30 pKa = 10.12QVFCDD35 pKa = 4.06GEE37 pKa = 4.13VDD39 pKa = 2.87EE40 pKa = 5.94DD41 pKa = 5.44YY42 pKa = 11.07YY43 pKa = 11.99VKK45 pKa = 10.75LIKK48 pKa = 10.44LDD50 pKa = 3.79VEE52 pKa = 3.94AFLYY56 pKa = 10.6DD57 pKa = 3.73KK58 pKa = 11.01YY59 pKa = 11.55DD60 pKa = 3.66KK61 pKa = 10.53EE62 pKa = 4.71TDD64 pKa = 4.31LSDD67 pKa = 3.64QLHH70 pKa = 7.14DD71 pKa = 3.53EE72 pKa = 4.48AEE74 pKa = 4.32TLKK77 pKa = 10.53EE78 pKa = 3.95YY79 pKa = 10.69HH80 pKa = 6.85LSLDD84 pKa = 3.61DD85 pKa = 5.17DD86 pKa = 3.94GTYY89 pKa = 9.22MVKK92 pKa = 10.24EE93 pKa = 4.11VAKK96 pKa = 10.76

Molecular weight: 11.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5D0E4|A0A0B5D0E4_9CAUD Uncharacterized protein OS=Listeria phage vB_LmoS_188 OX=1591072 GN=SE24_048 PE=4 SV=1
MM1 pKa = 7.43GKK3 pKa = 10.33YY4 pKa = 8.07YY5 pKa = 10.08WHH7 pKa = 6.92VSRR10 pKa = 11.84LGGKK14 pKa = 6.75PTEE17 pKa = 3.88IRR19 pKa = 11.84HH20 pKa = 5.44YY21 pKa = 10.3NHH23 pKa = 5.92ITKK26 pKa = 9.45MYY28 pKa = 10.54KK29 pKa = 10.34FILRR33 pKa = 11.84NPAMFKK39 pKa = 10.89DD40 pKa = 3.79KK41 pKa = 10.4TLTIYY46 pKa = 11.02DD47 pKa = 3.5HH48 pKa = 7.08AKK50 pKa = 10.36AVTNMTFNEE59 pKa = 3.72IKK61 pKa = 10.7YY62 pKa = 9.87RR63 pKa = 11.84ASLNLCEE70 pKa = 4.31TVEE73 pKa = 3.87RR74 pKa = 11.84RR75 pKa = 11.84YY76 pKa = 10.5VLSLTQRR83 pKa = 11.84LKK85 pKa = 11.07EE86 pKa = 4.04EE87 pKa = 4.13QAA89 pKa = 3.46

Molecular weight: 10.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

11853

41

1601

197.6

22.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.952 ± 0.559

0.633 ± 0.131

6.285 ± 0.249

7.914 ± 0.494

3.957 ± 0.291

5.712 ± 0.304

1.358 ± 0.183

6.69 ± 0.225

9.171 ± 0.42

8.032 ± 0.275

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.153

6.564 ± 0.282

2.759 ± 0.189

3.712 ± 0.24

3.577 ± 0.265

6.378 ± 0.269

6.378 ± 0.293

5.973 ± 0.26

1.147 ± 0.116

4.185 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski