Trichinella nativa
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16771 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0V1L8G2|A0A0V1L8G2_9BILA Uncharacterized protein OS=Trichinella nativa OX=6335 GN=T02_8157 PE=4 SV=1
MM1 pKa = 7.78 EE2 pKa = 5.25 DD3 pKa = 2.75 WKK5 pKa = 11.1 IFDD8 pKa = 4.19 FDD10 pKa = 3.79 VDD12 pKa = 3.85 LMLWLVMLAMLLHH25 pKa = 5.43 MAKK28 pKa = 10.42 YY29 pKa = 10.45 LLDD32 pKa = 3.73 YY33 pKa = 11.44 LEE35 pKa = 4.54 TFQEE39 pKa = 4.26 KK40 pKa = 10.83 EE41 pKa = 3.96 EE42 pKa = 4.85 LIQLLNEE49 pKa = 3.77 THH51 pKa = 6.37 YY52 pKa = 11.81 SMEE55 pKa = 3.9 QVEE58 pKa = 4.68 QIVEE62 pKa = 4.22 HH63 pKa = 5.41 QQSVDD68 pKa = 3.12 NKK70 pKa = 10.54 DD71 pKa = 4.01 YY72 pKa = 11.01 IGEE75 pKa = 4.15 PYY77 pKa = 10.21 IFYY80 pKa = 10.94 DD81 pKa = 3.27 PDD83 pKa = 4.24 KK84 pKa = 11.38 DD85 pKa = 4.21 VFTLQIDD92 pKa = 4.34 FNNLSEE98 pKa = 4.58 DD99 pKa = 3.47 NCIKK103 pKa = 10.69 VCLAVWNALVNIDD116 pKa = 5.08 SDD118 pKa = 4.86 SDD120 pKa = 4.78 DD121 pKa = 5.48 DD122 pKa = 6.7 DD123 pKa = 7.24 SDD125 pKa = 6.18 DD126 pKa = 5.14 DD127 pKa = 5.25 DD128 pKa = 6.09 SEE130 pKa = 4.73 EE131 pKa = 5.05 SDD133 pKa = 3.58 EE134 pKa = 4.16 QQ135 pKa = 3.8
Molecular weight: 16.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.541
IPC_protein 3.554
Toseland 3.325
ProMoST 3.732
Dawson 3.554
Bjellqvist 3.706
Wikipedia 3.503
Rodwell 3.376
Grimsley 3.236
Solomon 3.541
Lehninger 3.503
Nozaki 3.668
DTASelect 3.91
Thurlkill 3.389
EMBOSS 3.516
Sillero 3.681
Patrickios 0.985
IPC_peptide 3.528
IPC2_peptide 3.656
IPC2.peptide.svr19 3.668
Protein with the highest isoelectric point:
>tr|A0A0V1KIL0|A0A0V1KIL0_9BILA Uncharacterized protein (Fragment) OS=Trichinella nativa OX=6335 GN=T02_7353 PE=4 SV=1
LL1 pKa = 5.75 MQRR4 pKa = 11.84 KK5 pKa = 9.51 RR6 pKa = 11.84 GWSRR10 pKa = 11.84 LKK12 pKa = 10.31 RR13 pKa = 11.84 ARR15 pKa = 11.84 KK16 pKa = 8.95 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 8.45 KK20 pKa = 10.0 KK21 pKa = 7.73 MRR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 MRR27 pKa = 11.84 EE28 pKa = 3.39 TRR30 pKa = 11.84 RR31 pKa = 11.84 KK32 pKa = 10.2 RR33 pKa = 11.84 KK34 pKa = 7.82 TKK36 pKa = 9.79 LKK38 pKa = 11.15 KK39 pKa = 9.95 MMNN42 pKa = 3.65
Molecular weight: 5.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.474
IPC2_protein 11.052
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.559
Grimsley 12.837
Solomon 13.276
Lehninger 13.188
Nozaki 12.793
DTASelect 12.778
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.281
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.07
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13202
3569
16771
8159799
30
14853
486.5
55.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.44 ± 0.017
2.644 ± 0.018
5.266 ± 0.015
6.325 ± 0.026
4.582 ± 0.016
4.998 ± 0.019
2.468 ± 0.007
5.538 ± 0.015
5.995 ± 0.021
9.658 ± 0.021
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.409 ± 0.009
4.995 ± 0.013
4.355 ± 0.019
4.27 ± 0.013
5.648 ± 0.018
8.379 ± 0.02
5.296 ± 0.014
6.421 ± 0.012
1.217 ± 0.006
3.092 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here