Night heron coronavirus HKU19

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Deltacoronavirus; Herdecovirus

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9BR18|H9BR18_9NIDO Envelope protein OS=Night heron coronavirus HKU19 OX=1159904 GN=E PE=4 SV=1
MM1 pKa = 6.73QCVVLVLTLISIVTSRR17 pKa = 11.84PNSFADD23 pKa = 3.51RR24 pKa = 11.84VFDD27 pKa = 4.78ALTFPHH33 pKa = 7.18ASNYY37 pKa = 9.78LHH39 pKa = 6.9VGDD42 pKa = 4.81KK43 pKa = 10.41TPSRR47 pKa = 11.84PQLLQARR54 pKa = 11.84NQGNYY59 pKa = 9.86SAICPEE65 pKa = 3.77NGYY68 pKa = 8.28ITSTSYY74 pKa = 11.31DD75 pKa = 3.36LSKK78 pKa = 10.42IYY80 pKa = 10.82YY81 pKa = 8.25LTDD84 pKa = 2.93GDD86 pKa = 4.24YY87 pKa = 11.13PIDD90 pKa = 3.72GVYY93 pKa = 10.68KK94 pKa = 10.4SLQPLKK100 pKa = 9.37VTCVPEE106 pKa = 3.47WHH108 pKa = 6.96GNSNFNDD115 pKa = 3.54TTGWKK120 pKa = 9.78HH121 pKa = 5.67YY122 pKa = 10.19FDD124 pKa = 5.57GRR126 pKa = 11.84IKK128 pKa = 10.67QNPNTIWCPCSQSGPGGAQHH148 pKa = 6.51NAAGNSTEE156 pKa = 3.97YY157 pKa = 10.42IRR159 pKa = 11.84FHH161 pKa = 8.1SNITTSVSNLLRR173 pKa = 11.84LYY175 pKa = 10.86AVDD178 pKa = 3.05NQYY181 pKa = 11.55YY182 pKa = 9.72YY183 pKa = 10.6FGCTPTPTPLTFNLTSEE200 pKa = 4.54NITLFTAEE208 pKa = 4.3EE209 pKa = 4.07QVHH212 pKa = 5.27YY213 pKa = 10.34CYY215 pKa = 11.31ANINGTVSYY224 pKa = 10.54IGVLPPKK231 pKa = 9.51ITEE234 pKa = 4.24LTVGRR239 pKa = 11.84YY240 pKa = 9.59GDD242 pKa = 3.34IFVNGFLYY250 pKa = 9.95FKK252 pKa = 9.93IPNVIEE258 pKa = 4.06YY259 pKa = 9.73VQLSHH264 pKa = 7.46TIPHH268 pKa = 6.27NKK270 pKa = 8.81QFYY273 pKa = 8.24TVFYY277 pKa = 11.23ANMTQVLLNISMASINRR294 pKa = 11.84LLYY297 pKa = 10.13CDD299 pKa = 3.92KK300 pKa = 11.02DD301 pKa = 4.13SYY303 pKa = 11.86SSIACAVNQFEE314 pKa = 4.66PANGFYY320 pKa = 9.56STSAIEE326 pKa = 4.26KK327 pKa = 9.06ISRR330 pKa = 11.84KK331 pKa = 7.79FTFVTMPTVQNHH343 pKa = 5.75SYY345 pKa = 11.16YY346 pKa = 10.71SINLTIGGCGHH357 pKa = 6.92GEE359 pKa = 3.96YY360 pKa = 9.9PHH362 pKa = 7.01LSNKK366 pKa = 7.61TGCYY370 pKa = 8.57RR371 pKa = 11.84TDD373 pKa = 3.09ASNINAKK380 pKa = 10.34QITFVINTYY389 pKa = 7.58THH391 pKa = 7.18DD392 pKa = 3.78NWIQWAHH399 pKa = 6.52KK400 pKa = 9.87PGNCPWALNKK410 pKa = 9.97INNYY414 pKa = 7.36NTAGTLQVVPEE425 pKa = 4.13NQATCCTDD433 pKa = 3.35NQASWLYY440 pKa = 9.08LASWTSVNVKK450 pKa = 9.8VCFNYY455 pKa = 10.16QPGTTISIQPQQTGVATDD473 pKa = 3.25ISVIYY478 pKa = 10.42EE479 pKa = 4.17NEE481 pKa = 4.01CVDD484 pKa = 3.41YY485 pKa = 11.21NIYY488 pKa = 10.79GKK490 pKa = 8.52TGTGIIQSTNVTLLAGRR507 pKa = 11.84TYY509 pKa = 10.83TSASGQLLAFKK520 pKa = 10.28YY521 pKa = 10.57LSNQTIYY528 pKa = 11.19SVTPCDD534 pKa = 3.94FSNQVAVYY542 pKa = 8.52NKK544 pKa = 10.37SVIAAILPQNKK555 pKa = 9.01TIFGLTNIQEE565 pKa = 4.46TPNFYY570 pKa = 10.23IANNAHH576 pKa = 4.48QQQRR580 pKa = 11.84FAMYY584 pKa = 9.19MEE586 pKa = 5.27PLNSQQPDD594 pKa = 3.76CTPVLTYY601 pKa = 10.8AQIGICADD609 pKa = 3.51GQFVQVQPEE618 pKa = 4.1KK619 pKa = 9.64SQPMSTTPIVAVNITIPKK637 pKa = 7.65TFNISVQTEE646 pKa = 4.14YY647 pKa = 10.83IQISTDD653 pKa = 3.61NIVIDD658 pKa = 4.05CQRR661 pKa = 11.84YY662 pKa = 7.95VCNGNPRR669 pKa = 11.84CLMLLSQYY677 pKa = 10.88QSACSTIEE685 pKa = 3.73QALHH689 pKa = 4.66QKK691 pKa = 10.28ARR693 pKa = 11.84LEE695 pKa = 3.98SLEE698 pKa = 4.29LSTMLAYY705 pKa = 10.54SPNTLQLANVSNFQSNNMGFNLTNLLPQNNSPQKK739 pKa = 10.12RR740 pKa = 11.84SVIEE744 pKa = 4.18DD745 pKa = 3.59LLFSKK750 pKa = 10.81VVTNGLGTVDD760 pKa = 3.92VDD762 pKa = 4.09YY763 pKa = 11.28KK764 pKa = 11.13KK765 pKa = 9.82CTKK768 pKa = 10.11GLSIADD774 pKa = 4.3LPCAQYY780 pKa = 11.51YY781 pKa = 9.18NGIMVLPGVADD792 pKa = 3.69SGLLAAYY799 pKa = 7.24TASLTGGMVFGGLTSAAAIPFATAVQARR827 pKa = 11.84LNYY830 pKa = 9.72VALQTDD836 pKa = 3.94VLQRR840 pKa = 11.84NQQILANAFNQAMGNITLAFKK861 pKa = 10.39DD862 pKa = 3.66VKK864 pKa = 10.53EE865 pKa = 4.95AIATTADD872 pKa = 4.33AIRR875 pKa = 11.84VVAGALDD882 pKa = 4.87KK883 pKa = 10.83IQQVVNSQGQALSKK897 pKa = 10.15LTGEE901 pKa = 4.23LQRR904 pKa = 11.84NFQAISASIEE914 pKa = 4.2DD915 pKa = 3.63IYY917 pKa = 11.83NRR919 pKa = 11.84LNDD922 pKa = 3.64IEE924 pKa = 6.43ADD926 pKa = 3.62AQVDD930 pKa = 3.69RR931 pKa = 11.84LITGRR936 pKa = 11.84LAALNAFLTQTLTQANEE953 pKa = 3.69VKK955 pKa = 10.47AARR958 pKa = 11.84EE959 pKa = 3.65LALQKK964 pKa = 10.48INEE967 pKa = 4.38CVKK970 pKa = 10.66DD971 pKa = 3.42QSKK974 pKa = 10.82RR975 pKa = 11.84YY976 pKa = 7.99GFCGNGYY983 pKa = 9.62HH984 pKa = 7.05LFSIANAAPKK994 pKa = 10.69GFIFFHH1000 pKa = 6.07TVLQPEE1006 pKa = 4.43TTIEE1010 pKa = 3.8IQAIAGFCVSDD1021 pKa = 3.32RR1022 pKa = 11.84QTYY1025 pKa = 10.01NYY1027 pKa = 9.94YY1028 pKa = 10.08SSNMEE1033 pKa = 3.58GQAYY1037 pKa = 9.18IARR1040 pKa = 11.84DD1041 pKa = 3.53TTQTIFLHH1049 pKa = 6.37EE1050 pKa = 4.87NGTYY1054 pKa = 9.71MITPRR1059 pKa = 11.84KK1060 pKa = 8.96QYY1062 pKa = 9.8QPRR1065 pKa = 11.84TLAQADD1071 pKa = 3.8VVKK1074 pKa = 10.79ISTCDD1079 pKa = 3.13VTYY1082 pKa = 11.46VNLTSIEE1089 pKa = 4.1FEE1091 pKa = 4.05QLIPEE1096 pKa = 4.2YY1097 pKa = 11.05VDD1099 pKa = 3.03INSTVEE1105 pKa = 4.4GILNSTLPGKK1115 pKa = 10.37IPDD1118 pKa = 4.29LNIGHH1123 pKa = 6.4YY1124 pKa = 11.07NNTILNLTTEE1134 pKa = 4.25INDD1137 pKa = 3.86LQSKK1141 pKa = 10.57AEE1143 pKa = 4.12NLSMIAYY1150 pKa = 9.69QLEE1153 pKa = 4.4EE1154 pKa = 4.24YY1155 pKa = 10.04IKK1157 pKa = 10.76NINNTLVDD1165 pKa = 4.54LEE1167 pKa = 4.17WLNRR1171 pKa = 11.84VEE1173 pKa = 5.99TYY1175 pKa = 10.68LKK1177 pKa = 9.3WPWYY1181 pKa = 8.42VWLAIALAFTGFVTILITIFLCTGCCGGCFGCCGGCFGLFSKK1223 pKa = 10.66KK1224 pKa = 9.19IDD1226 pKa = 3.62PMRR1229 pKa = 11.84QYY1231 pKa = 10.23MNRR1234 pKa = 11.84YY1235 pKa = 5.86EE1236 pKa = 4.29TPTSKK1241 pKa = 10.83SDD1243 pKa = 3.39DD1244 pKa = 4.87AIPIIYY1250 pKa = 9.43KK1251 pKa = 10.59KK1252 pKa = 10.54NWW1254 pKa = 3.01

Molecular weight: 139.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9BR22|H9BR22_9NIDO NS7a protein OS=Night heron coronavirus HKU19 OX=1159904 GN=NS7a PE=4 SV=1
MM1 pKa = 7.58AVPRR5 pKa = 11.84VPRR8 pKa = 11.84ADD10 pKa = 3.59ASWFQPIKK18 pKa = 10.52AQNKK22 pKa = 9.59KK23 pKa = 8.02ITAPSFKK30 pKa = 10.65KK31 pKa = 10.62NGVPVNASLQAADD44 pKa = 3.35QHH46 pKa = 6.62GYY48 pKa = 6.39WLRR51 pKa = 11.84YY52 pKa = 9.34NRR54 pKa = 11.84TKK56 pKa = 10.79SGGGSIPAAFSFYY69 pKa = 9.81YY70 pKa = 10.29TGTGPYY76 pKa = 8.4ATLKK80 pKa = 10.76FGEE83 pKa = 4.58TPSDD87 pKa = 3.59RR88 pKa = 11.84ITWVKK93 pKa = 10.46GPSAVTTNKK102 pKa = 10.04PKK104 pKa = 10.09VAKK107 pKa = 9.82RR108 pKa = 11.84DD109 pKa = 3.82PDD111 pKa = 3.53KK112 pKa = 11.45HH113 pKa = 6.25PLLPLTFPPGDD124 pKa = 3.53GVNTGLRR131 pKa = 11.84VDD133 pKa = 4.14PFQNRR138 pKa = 11.84GRR140 pKa = 11.84TMDD143 pKa = 3.78RR144 pKa = 11.84GPNQRR149 pKa = 11.84SSSAPVDD156 pKa = 3.43SGNRR160 pKa = 11.84NQRR163 pKa = 11.84ARR165 pKa = 11.84SKK167 pKa = 10.38SAPSRR172 pKa = 11.84TNPKK176 pKa = 8.0THH178 pKa = 6.58QIPKK182 pKa = 8.71RR183 pKa = 11.84TLTKK187 pKa = 10.51GKK189 pKa = 8.71TISQVFGKK197 pKa = 8.61RR198 pKa = 11.84TPGGANVGSADD209 pKa = 3.67TEE211 pKa = 4.23KK212 pKa = 11.17AGFTDD217 pKa = 3.7PRR219 pKa = 11.84LMCLMRR225 pKa = 11.84HH226 pKa = 4.95TPGTQEE232 pKa = 4.33LLLAGHH238 pKa = 7.79LDD240 pKa = 3.59HH241 pKa = 7.89DD242 pKa = 4.09IQPEE246 pKa = 4.26GVYY249 pKa = 10.85LKK251 pKa = 9.3FTYY254 pKa = 7.52TTQVKK259 pKa = 9.81RR260 pKa = 11.84DD261 pKa = 3.29SAEE264 pKa = 3.74YY265 pKa = 10.68DD266 pKa = 3.37RR267 pKa = 11.84VIAALNNVVNQKK279 pKa = 10.27YY280 pKa = 10.11DD281 pKa = 3.59ADD283 pKa = 4.04NLPSQKK289 pKa = 10.13PKK291 pKa = 9.2STKK294 pKa = 9.88PKK296 pKa = 8.89PKK298 pKa = 9.77KK299 pKa = 9.22EE300 pKa = 4.16KK301 pKa = 10.4KK302 pKa = 9.48EE303 pKa = 3.79RR304 pKa = 11.84APPKK308 pKa = 9.48QQQKK312 pKa = 7.24EE313 pKa = 4.24QKK315 pKa = 9.74QEE317 pKa = 3.93PQHH320 pKa = 6.06EE321 pKa = 4.51SEE323 pKa = 4.5DD324 pKa = 3.68TKK326 pKa = 10.88KK327 pKa = 10.53IQWAEE332 pKa = 3.91DD333 pKa = 3.55DD334 pKa = 3.84TDD336 pKa = 4.1FAASFSS342 pKa = 3.85

Molecular weight: 37.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

8461

82

6280

1057.6

118.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.489 ± 0.421

2.565 ± 0.269

5.46 ± 0.632

3.735 ± 0.328

4.29 ± 0.233

5.035 ± 0.418

2.269 ± 0.256

6.288 ± 0.667

5.874 ± 0.701

8.982 ± 0.777

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.116 ± 0.299

6.217 ± 0.924

4.278 ± 0.679

4.16 ± 0.735

2.801 ± 0.485

6.56 ± 0.463

8.415 ± 0.263

8.013 ± 0.926

0.993 ± 0.471

5.46 ± 0.403
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski