Human endogenous retrovirus HCML-ARV
Average proteome isoelectric point is 7.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q7ZGS4|Q7ZGS4_9RETR Retroviral gag protein OS=Human endogenous retrovirus HCML-ARV OX=228277 PE=4 SV=1
MM1 pKa = 7.63 LKK3 pKa = 10.26 NFKK6 pKa = 10.36 KK7 pKa = 10.67 GFNGDD12 pKa = 3.62 YY13 pKa = 10.08 GVTMTPGKK21 pKa = 10.46 LRR23 pKa = 11.84 ILCEE27 pKa = 4.13 IDD29 pKa = 3.06 WPTLEE34 pKa = 4.62 VGWPSEE40 pKa = 3.91 GSLDD44 pKa = 3.4 RR45 pKa = 11.84 SLVSKK50 pKa = 10.13 VWHH53 pKa = 6.23 KK54 pKa = 9.68 VTGKK58 pKa = 10.11 SGHH61 pKa = 6.23 SDD63 pKa = 3.07 QFPYY67 pKa = 10.4 IDD69 pKa = 3.26 TWLLQLVQDD78 pKa = 4.23 PPQWLRR84 pKa = 11.84 GQAAAVLVAKK94 pKa = 10.01 GQIAKK99 pKa = 9.83 EE100 pKa = 4.55 GSRR103 pKa = 11.84 STHH106 pKa = 5.62 WGKK109 pKa = 8.47 STPEE113 pKa = 3.79 VLFDD117 pKa = 3.9 PTSEE121 pKa = 5.11 DD122 pKa = 3.82 PLQEE126 pKa = 4.18 MAPVIPVLPSPYY138 pKa = 9.24 QAEE141 pKa = 4.03 RR142 pKa = 11.84 LPTFEE147 pKa = 4.49 PTVLVPPQDD156 pKa = 3.16 KK157 pKa = 10.56 HH158 pKa = 7.02 IPRR161 pKa = 11.84 PPRR164 pKa = 11.84 VDD166 pKa = 2.92 KK167 pKa = 11.27 RR168 pKa = 11.84 GGEE171 pKa = 4.17 ASGEE175 pKa = 4.3 TPPLAACLRR184 pKa = 11.84 PKK186 pKa = 10.23 TGIQMPLRR194 pKa = 11.84 EE195 pKa = 3.95 QRR197 pKa = 11.84 YY198 pKa = 7.07 TGIEE202 pKa = 3.97 EE203 pKa = 4.52 DD204 pKa = 3.11 GHH206 pKa = 5.06 MVEE209 pKa = 5.01 KK210 pKa = 10.43 RR211 pKa = 11.84 VFVYY215 pKa = 10.58 QPFTSANLLNWKK227 pKa = 9.98 NNTLSYY233 pKa = 9.95 TEE235 pKa = 4.87 KK236 pKa = 10.24 PQALIDD242 pKa = 4.01 LLQTIIQTHH251 pKa = 5.78 NSTRR255 pKa = 11.84 ADD257 pKa = 3.52 CHH259 pKa = 5.93 QLLMFLFNTDD269 pKa = 2.09 EE270 pKa = 4.22 RR271 pKa = 11.84 QRR273 pKa = 11.84 VLQAATKK280 pKa = 8.79 WVQEE284 pKa = 4.27 HH285 pKa = 6.82 APADD289 pKa = 3.69 YY290 pKa = 10.76 QNPQEE295 pKa = 4.42 CVRR298 pKa = 11.84 TQLPGTDD305 pKa = 4.01 PQWDD309 pKa = 3.67 PNEE312 pKa = 4.31 RR313 pKa = 11.84 EE314 pKa = 3.87 DD315 pKa = 3.7 MQRR318 pKa = 11.84 LNRR321 pKa = 11.84 DD322 pKa = 3.0 RR323 pKa = 11.84 EE324 pKa = 4.26 AVLEE328 pKa = 3.97 GLKK331 pKa = 10.55 RR332 pKa = 11.84 GAQKK336 pKa = 9.47 ATNVNKK342 pKa = 9.77 VSEE345 pKa = 4.46 VIRR348 pKa = 11.84 GKK350 pKa = 10.51 EE351 pKa = 3.54 EE352 pKa = 4.0 SPAQFYY358 pKa = 10.81 QRR360 pKa = 11.84 LCEE363 pKa = 4.34 GYY365 pKa = 10.82 RR366 pKa = 11.84 MYY368 pKa = 11.01 TPFDD372 pKa = 3.52 PVSPEE377 pKa = 3.68 NQRR380 pKa = 11.84 MVNMALVSQSAEE392 pKa = 4.01 DD393 pKa = 3.97 IRR395 pKa = 11.84 RR396 pKa = 11.84 KK397 pKa = 9.24 LQKK400 pKa = 9.73 QDD402 pKa = 3.01 GFAGTNTSQLLEE414 pKa = 4.0 VANQVFVNRR423 pKa = 11.84 DD424 pKa = 3.02 AVSPKK429 pKa = 9.56 EE430 pKa = 3.75 NRR432 pKa = 11.84 RR433 pKa = 11.84 EE434 pKa = 3.87 NEE436 pKa = 3.47 RR437 pKa = 11.84 QARR440 pKa = 11.84 RR441 pKa = 11.84 NAEE444 pKa = 3.96 LLAAAVGGVSSKK456 pKa = 10.68 RR457 pKa = 11.84 QGKK460 pKa = 9.35 GGPGKK465 pKa = 8.6 EE466 pKa = 4.23 TQPGCQSLQCNQCAYY481 pKa = 10.08 CKK483 pKa = 10.51 EE484 pKa = 3.37 IGYY487 pKa = 8.55 WKK489 pKa = 10.42 NKK491 pKa = 9.25 CPQLKK496 pKa = 10.08 GKK498 pKa = 10.18 QGDD501 pKa = 4.11 LEE503 pKa = 4.37 QEE505 pKa = 4.3 VPDD508 pKa = 4.24 KK509 pKa = 11.65 EE510 pKa = 4.2 EE511 pKa = 4.06 GALLNLAEE519 pKa = 4.74 EE520 pKa = 4.63 LLDD523 pKa = 3.77
Molecular weight: 59.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.269
IPC2_protein 6.326
IPC_protein 6.389
Toseland 6.453
ProMoST 6.605
Dawson 6.766
Bjellqvist 6.751
Wikipedia 6.751
Rodwell 6.766
Grimsley 6.532
Solomon 6.781
Lehninger 6.781
Nozaki 7.044
DTASelect 7.146
Thurlkill 7.176
EMBOSS 7.161
Sillero 7.176
Patrickios 4.507
IPC_peptide 6.795
IPC2_peptide 6.825
IPC2.peptide.svr19 6.785
Protein with the highest isoelectric point:
>tr|Q7ZGS4|Q7ZGS4_9RETR Retroviral gag protein OS=Human endogenous retrovirus HCML-ARV OX=228277 PE=4 SV=1
MM1 pKa = 7.63 ARR3 pKa = 11.84 MTVGGKK9 pKa = 10.16 DD10 pKa = 2.84 IDD12 pKa = 4.04 FLVDD16 pKa = 3.28 TGAEE20 pKa = 4.15 HH21 pKa = 6.87 SVVTAPVAPLSKK33 pKa = 9.73 KK34 pKa = 9.21 TIDD37 pKa = 3.59 IIGAMGVSAKK47 pKa = 9.95 QAFCLPRR54 pKa = 11.84 TCTVGGHH61 pKa = 5.09 KK62 pKa = 10.27 VIHH65 pKa = 5.97 QFLYY69 pKa = 10.35 MPDD72 pKa = 3.85 CPLPLLGRR80 pKa = 11.84 DD81 pKa = 3.68 LLSKK85 pKa = 10.69 LRR87 pKa = 11.84 ATISFTEE94 pKa = 4.27 HH95 pKa = 6.48 GSLLLKK101 pKa = 10.92 LPGTGVIMTLTVPRR115 pKa = 11.84 EE116 pKa = 4.06 EE117 pKa = 4.03 EE118 pKa = 3.47 WRR120 pKa = 11.84 LFLTEE125 pKa = 4.16 SGQEE129 pKa = 3.76 IRR131 pKa = 11.84 PALAKK136 pKa = 9.96 RR137 pKa = 11.84 WPRR140 pKa = 11.84 VWAEE144 pKa = 4.13 DD145 pKa = 3.97 NPPGLAVNQAPVLIEE160 pKa = 4.16 VKK162 pKa = 10.47 PGAQPVRR169 pKa = 11.84 QKK171 pKa = 10.57 QYY173 pKa = 9.4 PVPRR177 pKa = 11.84 EE178 pKa = 3.98 ALEE181 pKa = 4.94 GIQVPLKK188 pKa = 10.17 CLRR191 pKa = 11.84 TFGMIVPCQSPWNTPLLPVPEE212 pKa = 5.37 PKK214 pKa = 10.23 TKK216 pKa = 10.41 DD217 pKa = 3.24 YY218 pKa = 11.13 WPGQDD223 pKa = 4.26 LRR225 pKa = 11.84 LVKK228 pKa = 10.3 QATVTLHH235 pKa = 6.24 PAVPNPYY242 pKa = 8.66 TLLGLLPAEE251 pKa = 4.96 DD252 pKa = 3.94 SCFTCLDD259 pKa = 3.54 LKK261 pKa = 11.19 DD262 pKa = 3.98 AFFSIRR268 pKa = 11.84 FAPEE272 pKa = 3.69 SQKK275 pKa = 10.98 LFAFQWEE282 pKa = 4.4 DD283 pKa = 3.47 PEE285 pKa = 5.34 SGVTTQYY292 pKa = 9.45 TWTWLPQGFKK302 pKa = 10.67 NSPTIFGEE310 pKa = 3.92 EE311 pKa = 4.24 LARR314 pKa = 11.84 DD315 pKa = 3.78 LQKK318 pKa = 11.19 CPTRR322 pKa = 11.84 DD323 pKa = 3.47 LGCVLLQYY331 pKa = 11.08 VDD333 pKa = 5.2 DD334 pKa = 5.23 LLLGHH339 pKa = 6.79 PTAVGCAKK347 pKa = 9.79 GTDD350 pKa = 3.06 ALLRR354 pKa = 11.84 HH355 pKa = 6.45 LEE357 pKa = 4.13 DD358 pKa = 3.96 CGYY361 pKa = 10.72 KK362 pKa = 10.05 VSKK365 pKa = 10.39 KK366 pKa = 9.79 KK367 pKa = 10.77 AQICRR372 pKa = 11.84 PQVRR376 pKa = 11.84 YY377 pKa = 10.19 LGFTIRR383 pKa = 11.84 QGEE386 pKa = 4.05 RR387 pKa = 11.84 SLGSEE392 pKa = 4.54 RR393 pKa = 11.84 KK394 pKa = 8.9 QVICTLPEE402 pKa = 4.11 PKK404 pKa = 9.78 SRR406 pKa = 11.84 KK407 pKa = 6.87 QVRR410 pKa = 11.84 KK411 pKa = 9.13 FLGAAGFCRR420 pKa = 11.84 LWIPNFAVLAKK431 pKa = 9.96 PLYY434 pKa = 10.18 EE435 pKa = 3.94 VTKK438 pKa = 10.04 WGDD441 pKa = 3.31 RR442 pKa = 11.84 EE443 pKa = 4.01 PFEE446 pKa = 4.96 WGSQQQQAFRR456 pKa = 11.84 EE457 pKa = 4.34 LKK459 pKa = 9.44 EE460 pKa = 4.27 KK461 pKa = 10.77 LMSAPALGLPNLTKK475 pKa = 10.63 PFTLYY480 pKa = 10.2 VSEE483 pKa = 4.24 RR484 pKa = 11.84 EE485 pKa = 4.05 KK486 pKa = 10.46 MAVRR490 pKa = 11.84 VLTQTVGPWPRR501 pKa = 11.84 PVAYY505 pKa = 10.33 LSKK508 pKa = 10.82 QLDD511 pKa = 4.2 GVSKK515 pKa = 10.42 GWPPCLRR522 pKa = 11.84 ALAATALLVQEE533 pKa = 4.5 AVKK536 pKa = 9.29 LTLGQNLNIKK546 pKa = 9.68 APHH549 pKa = 6.32 AMVTLINTKK558 pKa = 8.1 GHH560 pKa = 6.06 HH561 pKa = 6.14 WLTNARR567 pKa = 11.84 LTKK570 pKa = 10.08 YY571 pKa = 10.49 QSLLCEE577 pKa = 4.23 NPRR580 pKa = 11.84 ITIEE584 pKa = 3.6 ICNTLNPTTLLLVSEE599 pKa = 5.29 GPVEE603 pKa = 4.12 HH604 pKa = 7.47 DD605 pKa = 3.45 CVEE608 pKa = 4.29 VLDD611 pKa = 4.29 SVYY614 pKa = 10.96 SSRR617 pKa = 11.84 PDD619 pKa = 3.86 LQDD622 pKa = 3.45 QPWAPVDD629 pKa = 3.15 WEE631 pKa = 4.66 LYY633 pKa = 8.59 MDD635 pKa = 4.7 GGSFINPQGEE645 pKa = 4.03 RR646 pKa = 11.84 GAGYY650 pKa = 10.73 AVVTLDD656 pKa = 3.38 TVVEE660 pKa = 4.15 ARR662 pKa = 11.84 SLPQATSAQKK672 pKa = 10.89 AEE674 pKa = 4.68 LIAFIRR680 pKa = 11.84 ALEE683 pKa = 4.06 LSEE686 pKa = 4.83 GEE688 pKa = 4.43 TVNIYY693 pKa = 10.01 TDD695 pKa = 2.81 SRR697 pKa = 11.84 YY698 pKa = 9.87 PFLTLQVHH706 pKa = 5.21 EE707 pKa = 4.79 ASYY710 pKa = 10.98 KK711 pKa = 10.61 EE712 pKa = 3.97 KK713 pKa = 11.0 GLLNSGGKK721 pKa = 9.51 DD722 pKa = 2.53 IKK724 pKa = 10.48 YY725 pKa = 9.12 QQEE728 pKa = 3.77 ILQLLEE734 pKa = 4.96 AVWKK738 pKa = 7.04 PHH740 pKa = 5.46 KK741 pKa = 10.21 VAVMHH746 pKa = 6.47 CRR748 pKa = 11.84 GHH750 pKa = 5.39 QRR752 pKa = 11.84 ASTLVGLGNSRR763 pKa = 11.84 ADD765 pKa = 3.32 ARR767 pKa = 11.84 KK768 pKa = 9.04 AASAPFRR775 pKa = 11.84 ASVTAPLLPQAPDD788 pKa = 3.73 LLPTYY793 pKa = 10.67 SKK795 pKa = 11.0 EE796 pKa = 4.3 EE797 pKa = 3.94 KK798 pKa = 10.48 DD799 pKa = 3.53 FLQAEE804 pKa = 5.33 GGQVMEE810 pKa = 5.01 EE811 pKa = 3.95 GWIRR815 pKa = 11.84 LPDD818 pKa = 3.49 GRR820 pKa = 11.84 EE821 pKa = 3.79 AVPQLLGAAVVLAVHH836 pKa = 6.4 KK837 pKa = 6.85 TTHH840 pKa = 6.85 LGQEE844 pKa = 4.32 SLEE847 pKa = 4.21 KK848 pKa = 10.87 LLVRR852 pKa = 11.84 YY853 pKa = 8.39 FYY855 pKa = 10.5 ILHH858 pKa = 6.75 LSALAKK864 pKa = 9.76 TVTQRR869 pKa = 11.84 CVTCPKK875 pKa = 10.25 HH876 pKa = 5.98 NAKK879 pKa = 10.08 QGPAVPPVIQAYY891 pKa = 9.37 GAAPFEE897 pKa = 4.37 DD898 pKa = 3.74 VQVDD902 pKa = 4.04 FTEE905 pKa = 4.31 MPKK908 pKa = 10.71 CGGNKK913 pKa = 9.18 YY914 pKa = 10.7 LLVLVCTYY922 pKa = 10.9 SGGWRR927 pKa = 11.84 LIQHH931 pKa = 7.17 KK932 pKa = 9.93 LRR934 pKa = 11.84 KK935 pKa = 8.92 LVKK938 pKa = 8.68 LTCVLLRR945 pKa = 11.84 DD946 pKa = 4.64 PIPRR950 pKa = 11.84 FGLPLRR956 pKa = 11.84 IGSDD960 pKa = 3.05 NGPAFVADD968 pKa = 4.47 LVQKK972 pKa = 10.03 RR973 pKa = 11.84 AKK975 pKa = 10.59 VLGNTWKK982 pKa = 10.35 LHH984 pKa = 4.82 AAYY987 pKa = 10.0 RR988 pKa = 11.84 PQSSGKK994 pKa = 9.05 VEE996 pKa = 3.87 QMNWTIKK1003 pKa = 9.43 NSKK1006 pKa = 10.34 GKK1008 pKa = 9.72 VCQEE1012 pKa = 4.39 TGLKK1016 pKa = 9.21 WIQALPMVLFKK1027 pKa = 10.57 IRR1029 pKa = 11.84 CTPSKK1034 pKa = 9.46 RR1035 pKa = 11.84 TGYY1038 pKa = 10.04 SPYY1041 pKa = 9.84 EE1042 pKa = 4.04 ILYY1045 pKa = 9.54 HH1046 pKa = 6.5 RR1047 pKa = 11.84 PPPILRR1053 pKa = 11.84 GLPGTPRR1060 pKa = 11.84 EE1061 pKa = 4.07 LGEE1064 pKa = 5.09 IEE1066 pKa = 5.17 LQRR1069 pKa = 11.84 QLQALGKK1076 pKa = 8.06 ITQTISAWVNEE1087 pKa = 4.32 RR1088 pKa = 11.84 CPVSLFSPVHH1098 pKa = 5.89 PFSPGNRR1105 pKa = 11.84 VWIKK1109 pKa = 9.86 DD1110 pKa = 3.33 WNVASLCPRR1119 pKa = 11.84 WKK1121 pKa = 10.69 GPQTVILTTPTAVKK1135 pKa = 10.8 VEE1137 pKa = 4.4 GVPAWIHH1144 pKa = 5.39 HH1145 pKa = 5.76 SRR1147 pKa = 11.84 VKK1149 pKa = 10.31 PAVPEE1154 pKa = 4.03 TSEE1157 pKa = 4.0 VRR1159 pKa = 11.84 PSPEE1163 pKa = 3.84 DD1164 pKa = 3.32 PCRR1167 pKa = 11.84 VTLKK1171 pKa = 9.54 KK1172 pKa = 7.36 TTSPAPVTPGSS1183 pKa = 3.42
Molecular weight: 131.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.553
IPC2_protein 8.419
IPC_protein 8.229
Toseland 8.741
ProMoST 8.843
Dawson 9.224
Bjellqvist 9.224
Wikipedia 9.311
Rodwell 9.399
Grimsley 9.063
Solomon 9.253
Lehninger 9.224
Nozaki 9.443
DTASelect 9.048
Thurlkill 9.18
EMBOSS 9.385
Sillero 9.385
Patrickios 4.749
IPC_peptide 9.238
IPC2_peptide 8.009
IPC2.peptide.svr19 7.756
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2388
523
1183
796.0
89.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.658 ± 0.612
2.596 ± 0.334
3.727 ± 0.403
6.03 ± 0.656
3.015 ± 0.275
6.826 ± 0.039
2.052 ± 0.179
4.523 ± 1.097
6.407 ± 0.032
10.469 ± 0.849
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.34 ± 0.195
3.601 ± 0.834
7.58 ± 0.79
5.695 ± 0.752
5.486 ± 0.431
4.899 ± 0.044
6.826 ± 0.675
7.161 ± 0.492
2.387 ± 0.234
2.722 ± 0.322
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here