Streptomyces sp. CB03911

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7703 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q4Y8L8|A0A1Q4Y8L8_9ACTN Uncharacterized protein OS=Streptomyces sp. CB03911 OX=1804758 GN=A6A07_01410 PE=4 SV=1
MM1 pKa = 7.42SVTGEE6 pKa = 3.7ATAAGEE12 pKa = 4.0PLEE15 pKa = 4.19VWIDD19 pKa = 3.44QDD21 pKa = 3.99LCTGDD26 pKa = 4.65GICVQYY32 pKa = 10.96APEE35 pKa = 4.18VFEE38 pKa = 5.9LDD40 pKa = 3.05IDD42 pKa = 3.65GLAYY46 pKa = 10.7VKK48 pKa = 10.81GADD51 pKa = 4.38DD52 pKa = 4.89EE53 pKa = 5.64LRR55 pKa = 11.84QQPGDD60 pKa = 3.65TAPVPLTLLQDD71 pKa = 3.88VVDD74 pKa = 4.41SAKK77 pKa = 10.25EE78 pKa = 3.92CPGDD82 pKa = 4.18CIHH85 pKa = 6.28VRR87 pKa = 11.84RR88 pKa = 11.84VADD91 pKa = 3.62RR92 pKa = 11.84VEE94 pKa = 4.48VYY96 pKa = 10.88GPDD99 pKa = 3.33ADD101 pKa = 3.69

Molecular weight: 10.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q4WP50|A0A1Q4WP50_9ACTN Uncharacterized protein OS=Streptomyces sp. CB03911 OX=1804758 GN=A6A07_21410 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.69GRR40 pKa = 11.84TNISAA45 pKa = 3.72

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7703

0

7703

2525808

37

8016

327.9

34.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.49 ± 0.043

0.754 ± 0.008

5.728 ± 0.022

5.32 ± 0.03

2.674 ± 0.015

9.867 ± 0.03

2.198 ± 0.014

2.945 ± 0.019

1.865 ± 0.024

10.703 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.548 ± 0.011

1.78 ± 0.02

6.457 ± 0.032

2.925 ± 0.016

7.801 ± 0.033

4.927 ± 0.023

6.167 ± 0.027

8.323 ± 0.026

1.52 ± 0.013

2.009 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski