Ctenophore-associated circular virus 4
Average proteome isoelectric point is 8.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A141MJB2|A0A141MJB2_9VIRU Coat protein OS=Ctenophore-associated circular virus 4 OX=1778561 PE=4 SV=1
MM1 pKa = 7.64 TEE3 pKa = 3.9 PTQQDD8 pKa = 3.41 TAGNSGGNTKK18 pKa = 10.13 GKK20 pKa = 9.69 RR21 pKa = 11.84 RR22 pKa = 11.84 CFAFTWNNYY31 pKa = 8.37 PEE33 pKa = 4.13 EE34 pKa = 4.21 HH35 pKa = 6.57 FKK37 pKa = 11.4 LFDD40 pKa = 3.48 TVAQLSGCKK49 pKa = 9.07 FVYY52 pKa = 10.26 QEE54 pKa = 3.97 EE55 pKa = 4.33 TGEE58 pKa = 4.11 NGTKK62 pKa = 10.13 HH63 pKa = 5.93 LQGALYY69 pKa = 10.32 FEE71 pKa = 5.2 NARR74 pKa = 11.84 SWTSVRR80 pKa = 11.84 KK81 pKa = 10.14 LMDD84 pKa = 3.32 GWHH87 pKa = 6.65 LEE89 pKa = 4.12 VARR92 pKa = 11.84 NWMACVKK99 pKa = 10.13 YY100 pKa = 9.05 ATKK103 pKa = 10.78 VEE105 pKa = 4.21 TRR107 pKa = 11.84 TGDD110 pKa = 3.11 QYY112 pKa = 11.96 SNFLEE117 pKa = 4.62 LRR119 pKa = 11.84 PKK121 pKa = 10.75 DD122 pKa = 3.61 PLEE125 pKa = 4.34 GKK127 pKa = 9.42 VLHH130 pKa = 6.66 PWQSDD135 pKa = 3.53 VMNLISSDD143 pKa = 3.61 CSNDD147 pKa = 3.25 RR148 pKa = 11.84 TVHH151 pKa = 5.4 WFVDD155 pKa = 3.69 SEE157 pKa = 4.46 GAKK160 pKa = 10.6 GKK162 pKa = 8.94 TSLCKK167 pKa = 10.29 HH168 pKa = 5.7 ILLNKK173 pKa = 9.59 VGWLFISAGKK183 pKa = 10.6 GGDD186 pKa = 3.32 ILCAVARR193 pKa = 11.84 YY194 pKa = 8.82 LKK196 pKa = 10.45 SGKK199 pKa = 9.53 KK200 pKa = 9.77 LRR202 pKa = 11.84 GVLANYY208 pKa = 10.32 SRR210 pKa = 11.84 DD211 pKa = 3.42 KK212 pKa = 11.15 EE213 pKa = 4.41 GFISYY218 pKa = 10.21 SALEE222 pKa = 4.22 SIKK225 pKa = 10.96 DD226 pKa = 3.53 GLIFSTKK233 pKa = 10.14 YY234 pKa = 10.04 EE235 pKa = 4.15 SQHH238 pKa = 7.27 AIFDD242 pKa = 4.19 SPHH245 pKa = 6.18 VICFANWIPDD255 pKa = 3.39 KK256 pKa = 11.07 RR257 pKa = 11.84 KK258 pKa = 10.36 LSDD261 pKa = 3.73 DD262 pKa = 3.12 RR263 pKa = 11.84 WDD265 pKa = 3.2 IRR267 pKa = 11.84 TLL269 pKa = 3.45
Molecular weight: 30.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.493
IPC2_protein 7.819
IPC_protein 7.702
Toseland 7.424
ProMoST 8.244
Dawson 8.434
Bjellqvist 8.726
Wikipedia 8.361
Rodwell 8.463
Grimsley 7.424
Solomon 8.536
Lehninger 8.551
Nozaki 8.96
DTASelect 8.478
Thurlkill 8.551
EMBOSS 8.639
Sillero 8.858
Patrickios 4.507
IPC_peptide 8.536
IPC2_peptide 7.6
IPC2.peptide.svr19 7.78
Protein with the highest isoelectric point:
>tr|A0A141MJB2|A0A141MJB2_9VIRU Coat protein OS=Ctenophore-associated circular virus 4 OX=1778561 PE=4 SV=1
MM1 pKa = 7.12 VRR3 pKa = 11.84 KK4 pKa = 7.74 TKK6 pKa = 10.62 KK7 pKa = 9.79 KK8 pKa = 10.25 GKK10 pKa = 9.46 QLVTKK15 pKa = 10.25 QYY17 pKa = 11.68 LNAKK21 pKa = 8.55 LNKK24 pKa = 10.14 AIEE27 pKa = 4.12 TKK29 pKa = 10.64 YY30 pKa = 10.84 YY31 pKa = 7.89 YY32 pKa = 10.14 TLGASSPDD40 pKa = 3.35 TGGSLVTVSNGIAQGDD56 pKa = 4.22 AYY58 pKa = 10.97 NEE60 pKa = 4.01 RR61 pKa = 11.84 LGDD64 pKa = 4.38 RR65 pKa = 11.84 ITPIHH70 pKa = 6.03 CHH72 pKa = 5.49 IKK74 pKa = 9.65 GTVTLADD81 pKa = 3.28 TTNWVRR87 pKa = 11.84 IVLIRR92 pKa = 11.84 WKK94 pKa = 10.17 QDD96 pKa = 3.18 YY97 pKa = 11.46 GDD99 pKa = 4.27 LTDD102 pKa = 4.3 ANQIFEE108 pKa = 4.46 ATGATTSPYY117 pKa = 10.58 SLFVKK122 pKa = 10.44 EE123 pKa = 4.02 PDD125 pKa = 2.87 NRR127 pKa = 11.84 ARR129 pKa = 11.84 FDD131 pKa = 3.49 VLADD135 pKa = 3.62 RR136 pKa = 11.84 LFNLHH141 pKa = 6.65 SNEE144 pKa = 3.89 PQKK147 pKa = 11.38 AFDD150 pKa = 3.59 IRR152 pKa = 11.84 VSSRR156 pKa = 11.84 KK157 pKa = 8.87 LHH159 pKa = 5.58 SRR161 pKa = 11.84 PVSFTAGGSGGNGQLVMVTISDD183 pKa = 4.01 SAAVAHH189 pKa = 6.29 PSISYY194 pKa = 11.12 YY195 pKa = 11.5 LMLKK199 pKa = 10.48 YY200 pKa = 10.34 KK201 pKa = 10.57 DD202 pKa = 3.42 AA203 pKa = 5.65
Molecular weight: 22.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.872
IPC2_protein 9.063
IPC_protein 8.99
Toseland 9.619
ProMoST 9.385
Dawson 9.897
Bjellqvist 9.575
Wikipedia 10.072
Rodwell 10.204
Grimsley 9.97
Solomon 9.926
Lehninger 9.882
Nozaki 9.619
DTASelect 9.575
Thurlkill 9.721
EMBOSS 10.043
Sillero 9.794
Patrickios 5.13
IPC_peptide 9.911
IPC2_peptide 8.126
IPC2.peptide.svr19 8.017
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
472
203
269
236.0
26.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.992 ± 0.834
1.695 ± 0.725
6.356 ± 0.029
4.237 ± 1.07
4.025 ± 0.645
7.627 ± 0.144
2.754 ± 0.176
4.661 ± 0.457
8.263 ± 0.23
8.475 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.483 ± 0.003
4.873 ± 0.032
2.966 ± 0.291
3.178 ± 0.163
5.297 ± 0.074
7.839 ± 0.026
6.992 ± 0.834
5.932 ± 0.582
2.331 ± 0.811
4.025 ± 0.543
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here