Escherichia phage Skarpretter

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Skarprettervirus; Escherichia virus Skarpretter

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8F3F6|A0A3G8F3F6_9CAUD Uncharacterized protein OS=Escherichia phage Skarpretter OX=2488654 PE=4 SV=1
MM1 pKa = 7.99DD2 pKa = 6.54EE3 pKa = 4.08ITISIIDD10 pKa = 3.65QLADD14 pKa = 3.46GVPFYY19 pKa = 11.24DD20 pKa = 4.64LLSGYY25 pKa = 10.39DD26 pKa = 3.77SEE28 pKa = 5.98SLIQEE33 pKa = 4.51LAQRR37 pKa = 11.84LRR39 pKa = 11.84DD40 pKa = 3.73ANDD43 pKa = 3.72EE44 pKa = 4.17IEE46 pKa = 4.31SLGYY50 pKa = 10.5EE51 pKa = 4.2VMEE54 pKa = 4.54LQDD57 pKa = 4.41PGSSS61 pKa = 3.19

Molecular weight: 6.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8F305|A0A3G8F305_9CAUD Uncharacterized protein OS=Escherichia phage Skarpretter OX=2488654 PE=4 SV=1
MM1 pKa = 7.63TSTTPLAAAIRR12 pKa = 11.84SSLPAEE18 pKa = 4.71PYY20 pKa = 10.25DD21 pKa = 3.47PAVAGEE27 pKa = 3.98VRR29 pKa = 11.84KK30 pKa = 10.38IMARR34 pKa = 11.84DD35 pKa = 3.3AGITGNRR42 pKa = 11.84AAMQRR47 pKa = 11.84SLTGYY52 pKa = 9.14AQAEE56 pKa = 4.09EE57 pKa = 4.58TEE59 pKa = 4.4RR60 pKa = 11.84EE61 pKa = 3.84RR62 pKa = 11.84KK63 pKa = 9.49ARR65 pKa = 11.84LIARR69 pKa = 11.84GTVRR73 pKa = 11.84LMSMGLTRR81 pKa = 11.84KK82 pKa = 8.71QAEE85 pKa = 4.09KK86 pKa = 9.64VARR89 pKa = 11.84RR90 pKa = 11.84SYY92 pKa = 11.15HH93 pKa = 6.42LAKK96 pKa = 10.13QDD98 pKa = 3.31AKK100 pKa = 11.33AA101 pKa = 3.56

Molecular weight: 11.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

13179

36

977

209.2

23.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.602 ± 0.565

1.138 ± 0.19

6.457 ± 0.227

5.964 ± 0.382

3.119 ± 0.17

7.922 ± 0.37

1.692 ± 0.2

5.129 ± 0.29

5.805 ± 0.354

7.072 ± 0.274

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.724 ± 0.146

4.553 ± 0.256

4.538 ± 0.233

4.917 ± 0.47

5.812 ± 0.22

4.636 ± 0.19

5.858 ± 0.3

6.199 ± 0.222

1.556 ± 0.149

3.308 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski