Altererythrobacter insulae
Average proteome isoelectric point is 5.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2967 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A411Z8T7|A0A411Z8T7_9SPHN Multifunctional CCA protein OS=Altererythrobacter insulae OX=2169535 GN=BPTFM16_02798 PE=4 SV=1
MM1 pKa = 8.06 LDD3 pKa = 3.75 PLLLPMNSAVDD14 pKa = 4.07 GMQCLYY20 pKa = 10.3 IDD22 pKa = 4.61 DD23 pKa = 4.11 MSANFLRR30 pKa = 11.84 HH31 pKa = 5.61 GNPVQAYY38 pKa = 7.13 NAPAEE43 pKa = 4.37 GSVQVYY49 pKa = 10.1 IGEE52 pKa = 4.68 DD53 pKa = 3.48 EE54 pKa = 4.22 NDD56 pKa = 3.39 ANAEE60 pKa = 4.16 FIGVGFINDD69 pKa = 4.46 DD70 pKa = 3.42 GLLAPKK76 pKa = 9.99 RR77 pKa = 11.84 IVVII81 pKa = 4.34
Molecular weight: 8.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.821
IPC_protein 3.745
Toseland 3.541
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.719
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.681
Nozaki 3.884
DTASelect 4.113
Thurlkill 3.617
EMBOSS 3.732
Sillero 3.872
Patrickios 1.888
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.773
Protein with the highest isoelectric point:
>tr|A0A411ZA29|A0A411ZA29_9SPHN Phosphoenolpyruvate--protein phosphotransferase OS=Altererythrobacter insulae OX=2169535 GN=ptsP PE=3 SV=1
MM1 pKa = 7.11 QKK3 pKa = 9.97 QVAPQAKK10 pKa = 9.11 LAAGTMRR17 pKa = 11.84 APQWVLLIALLLGNAALATGPWLVRR42 pKa = 11.84 LADD45 pKa = 3.74 TGSVSAAFWRR55 pKa = 11.84 LFLAIPFLLIFARR68 pKa = 11.84 LAGQRR73 pKa = 11.84 IGGMPKK79 pKa = 8.57 RR80 pKa = 11.84 TLVFVAIGASAFAMDD95 pKa = 4.77 LASWHH100 pKa = 6.67 IGIGMTRR107 pKa = 11.84 LGNATLFGNAGSIILLFWGFVIARR131 pKa = 11.84 MLPQKK136 pKa = 10.44 FEE138 pKa = 3.88 WLAIFCALSGAAILLGRR155 pKa = 11.84 SLEE158 pKa = 4.06 ISTTTFIGDD167 pKa = 4.66 LFCLAAGVLYY177 pKa = 10.42 AVYY180 pKa = 10.93 LLTLQGARR188 pKa = 11.84 AKK190 pKa = 10.36 FGNWSLLAWVSIFASPLLLALALALGEE217 pKa = 4.52 PVWPTNWTPILILFVTSQLIGQGLLVFSLRR247 pKa = 11.84 HH248 pKa = 5.52 FPPLVIGLALLTQPAVAALIGYY270 pKa = 7.35 QVFGEE275 pKa = 4.3 VLLPLDD281 pKa = 3.88 ILGMALLGSALVLARR296 pKa = 11.84 KK297 pKa = 9.8 SNARR301 pKa = 11.84 PVNPP305 pKa = 4.33
Molecular weight: 32.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.34
IPC2_protein 9.765
IPC_protein 10.716
Toseland 10.847
ProMoST 10.643
Dawson 10.935
Bjellqvist 10.687
Wikipedia 11.169
Rodwell 11.082
Grimsley 10.979
Solomon 11.096
Lehninger 11.067
Nozaki 10.847
DTASelect 10.672
Thurlkill 10.847
EMBOSS 11.272
Sillero 10.877
Patrickios 10.833
IPC_peptide 11.111
IPC2_peptide 9.823
IPC2.peptide.svr19 8.526
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2967
0
2967
877807
29
4416
295.9
32.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.678 ± 0.06
0.822 ± 0.013
6.279 ± 0.049
6.574 ± 0.047
3.812 ± 0.03
8.463 ± 0.057
1.928 ± 0.026
5.481 ± 0.033
3.645 ± 0.045
9.581 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.623 ± 0.024
3.054 ± 0.026
4.802 ± 0.03
3.363 ± 0.025
6.246 ± 0.051
5.795 ± 0.033
5.248 ± 0.046
6.915 ± 0.035
1.407 ± 0.02
2.285 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here