Capybara microvirus Cap3_SP_333
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W563|A0A4P8W563_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_333 OX=2585431 PE=4 SV=1
MM1 pKa = 7.53 IIFYY5 pKa = 10.69 VFLHH9 pKa = 5.99 IKK11 pKa = 9.54 IFNAMLFYY19 pKa = 11.11 KK20 pKa = 10.34 LFIEE24 pKa = 5.89 FSSQTDD30 pKa = 3.67 SFNEE34 pKa = 4.18 EE35 pKa = 3.75 YY36 pKa = 10.71 DD37 pKa = 3.61 FSSFEE42 pKa = 4.04 DD43 pKa = 3.57 AVAYY47 pKa = 10.6 LSDD50 pKa = 3.61 IASHH54 pKa = 6.9 FLITNVKK61 pKa = 8.9 IDD63 pKa = 3.81 SYY65 pKa = 11.88 YY66 pKa = 10.99 EE67 pKa = 3.88 DD68 pKa = 3.57
Molecular weight: 8.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.953
IPC2_protein 4.164
IPC_protein 4.037
Toseland 3.846
ProMoST 4.075
Dawson 4.012
Bjellqvist 4.253
Wikipedia 3.948
Rodwell 3.872
Grimsley 3.77
Solomon 3.999
Lehninger 3.961
Nozaki 4.151
DTASelect 4.329
Thurlkill 3.91
EMBOSS 3.961
Sillero 4.151
Patrickios 0.477
IPC_peptide 3.999
IPC2_peptide 4.139
IPC2.peptide.svr19 4.12
Protein with the highest isoelectric point:
>tr|A0A4P8W8D2|A0A4P8W8D2_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_333 OX=2585431 PE=4 SV=1
MM1 pKa = 6.81 EE2 pKa = 5.94 TINKK6 pKa = 8.92 FLDD9 pKa = 3.35 WLRR12 pKa = 11.84 GLPIWVRR19 pKa = 11.84 AVACLLAAAAIFLISLQSCSSVRR42 pKa = 11.84 VVGNTADD49 pKa = 3.16 SRR51 pKa = 11.84 VTVRR55 pKa = 11.84 QSALDD60 pKa = 3.54 STRR63 pKa = 11.84 ITIDD67 pKa = 2.97 VLPNFNVNKK76 pKa = 10.46
Molecular weight: 8.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.824
IPC2_protein 8.492
IPC_protein 8.829
Toseland 9.399
ProMoST 9.619
Dawson 9.458
Bjellqvist 9.487
Wikipedia 9.443
Rodwell 9.648
Grimsley 8.975
Solomon 9.882
Lehninger 9.911
Nozaki 9.926
DTASelect 9.282
Thurlkill 9.487
EMBOSS 9.75
Sillero 9.706
Patrickios 10.292
IPC_peptide 9.867
IPC2_peptide 8.873
IPC2.peptide.svr19 8.192
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1902
68
661
271.7
30.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.204 ± 1.216
1.577 ± 0.472
6.94 ± 0.402
4.154 ± 0.481
6.625 ± 1.01
5.205 ± 0.59
2.05 ± 0.695
5.994 ± 0.247
4.469 ± 0.334
9.464 ± 0.598
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.788 ± 0.131
4.837 ± 0.598
3.785 ± 0.741
5.363 ± 1.398
5.1 ± 0.513
9.937 ± 0.544
4.732 ± 0.288
5.678 ± 0.294
1.262 ± 0.168
4.837 ± 0.57
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here