Naegleria fowleri (Brain eating amoeba)

Taxonomy: cellular organisms; Eukaryota; Discoba; Heterolobosea; Tetramitia; Eutetramitia; Vahlkampfiidae; Naegleria

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13596 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6A5AU86|A0A6A5AU86_NAEFO RING-type domain-containing protein OS=Naegleria fowleri OX=5763 GN=FDP41_010076 PE=4 SV=1
MM1 pKa = 7.75DD2 pKa = 4.38LWYY5 pKa = 10.38CEE7 pKa = 4.33KK8 pKa = 10.45EE9 pKa = 3.96IEE11 pKa = 4.53SADD14 pKa = 4.31RR15 pKa = 11.84MIEE18 pKa = 3.73QWKK21 pKa = 8.21TEE23 pKa = 3.76IGEE26 pKa = 4.31GFDD29 pKa = 3.53VNFMRR34 pKa = 11.84FEE36 pKa = 4.34LLSEE40 pKa = 4.21SLKK43 pKa = 10.7HH44 pKa = 5.61IQRR47 pKa = 11.84EE48 pKa = 4.3DD49 pKa = 3.23EE50 pKa = 4.84FLNLSNNGYY59 pKa = 10.3CDD61 pKa = 4.95DD62 pKa = 6.25DD63 pKa = 4.4EE64 pKa = 5.04EE65 pKa = 6.52CDD67 pKa = 5.05DD68 pKa = 5.44EE69 pKa = 4.91EE70 pKa = 4.52EE71 pKa = 4.41CDD73 pKa = 3.84YY74 pKa = 11.74EE75 pKa = 4.28EE76 pKa = 4.61YY77 pKa = 10.47EE78 pKa = 4.2YY79 pKa = 11.42SRR81 pKa = 11.84DD82 pKa = 3.55CATPISPAACEE93 pKa = 3.89YY94 pKa = 10.52FSFVSEE100 pKa = 4.47CSDD103 pKa = 5.12DD104 pKa = 3.87EE105 pKa = 6.56DD106 pKa = 4.05EE107 pKa = 5.5DD108 pKa = 4.42EE109 pKa = 4.9YY110 pKa = 11.98YY111 pKa = 10.94SDD113 pKa = 6.08DD114 pKa = 4.12EE115 pKa = 5.85DD116 pKa = 5.66DD117 pKa = 4.78EE118 pKa = 5.15EE119 pKa = 6.01DD120 pKa = 5.33FEE122 pKa = 5.87EE123 pKa = 4.9EE124 pKa = 4.49DD125 pKa = 3.36NCVYY129 pKa = 11.46DD130 pKa = 4.68NMDD133 pKa = 3.94TVSVICDD140 pKa = 3.36QNHH143 pKa = 6.57PNGNSIEE150 pKa = 4.29DD151 pKa = 3.69TTADD155 pKa = 3.52NSEE158 pKa = 4.58KK159 pKa = 10.66GHH161 pKa = 5.72PHH163 pKa = 5.49QDD165 pKa = 2.8ASNKK169 pKa = 9.28HH170 pKa = 5.44MKK172 pKa = 9.17TFSRR176 pKa = 11.84DD177 pKa = 3.58LIHH180 pKa = 7.67DD181 pKa = 4.31EE182 pKa = 4.41YY183 pKa = 10.94IGGLLHH189 pKa = 7.15EE190 pKa = 5.71FGPCSTHH197 pKa = 7.53SNTASNKK204 pKa = 9.98LQMDD208 pKa = 3.67DD209 pKa = 4.05HH210 pKa = 7.18EE211 pKa = 4.99EE212 pKa = 4.27GQEE215 pKa = 4.02EE216 pKa = 4.92EE217 pKa = 4.55PSSPQPIMISNCDD230 pKa = 3.48TLSEE234 pKa = 4.27QEE236 pKa = 3.95MSSINSSDD244 pKa = 3.44NEE246 pKa = 4.33TSHH249 pKa = 7.41SCIVNNKK256 pKa = 5.24QTVDD260 pKa = 3.98FSKK263 pKa = 9.61HH264 pKa = 3.57QQVIFEE270 pKa = 4.25

Molecular weight: 31.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6A5C3D5|A0A6A5C3D5_NAEFO Guanylate cyclase domain-containing protein OS=Naegleria fowleri OX=5763 GN=FDP41_000812 PE=4 SV=1
MM1 pKa = 7.49NKK3 pKa = 10.1FILLALAMLFCMVMFVATTTTQAASADD30 pKa = 3.74TLAASTLCFGLKK42 pKa = 9.42VKK44 pKa = 10.57RR45 pKa = 11.84NRR47 pKa = 11.84RR48 pKa = 11.84ATCIRR53 pKa = 11.84RR54 pKa = 11.84HH55 pKa = 5.7RR56 pKa = 11.84KK57 pKa = 8.64LLRR60 pKa = 11.84RR61 pKa = 11.84VPSKK65 pKa = 9.96RR66 pKa = 11.84RR67 pKa = 11.84AVIRR71 pKa = 11.84RR72 pKa = 11.84LLKK75 pKa = 8.81RR76 pKa = 11.84CRR78 pKa = 11.84RR79 pKa = 11.84NRR81 pKa = 11.84RR82 pKa = 11.84AVKK85 pKa = 10.14KK86 pKa = 10.06ALKK89 pKa = 9.97RR90 pKa = 11.84SRR92 pKa = 11.84KK93 pKa = 7.13LARR96 pKa = 11.84RR97 pKa = 11.84IRR99 pKa = 11.84RR100 pKa = 11.84VKK102 pKa = 10.12SKK104 pKa = 10.0RR105 pKa = 11.84ARR107 pKa = 11.84RR108 pKa = 11.84AIRR111 pKa = 11.84RR112 pKa = 11.84ALKK115 pKa = 9.89RR116 pKa = 11.84ARR118 pKa = 11.84RR119 pKa = 11.84QAKK122 pKa = 9.54KK123 pKa = 9.46IRR125 pKa = 11.84RR126 pKa = 11.84AARR129 pKa = 11.84RR130 pKa = 11.84CVKK133 pKa = 10.22RR134 pKa = 11.84ASKK137 pKa = 10.31KK138 pKa = 9.26ARR140 pKa = 11.84KK141 pKa = 8.91ARR143 pKa = 11.84KK144 pKa = 8.0LARR147 pKa = 11.84RR148 pKa = 11.84RR149 pKa = 11.84ARR151 pKa = 11.84RR152 pKa = 11.84AARR155 pKa = 11.84KK156 pKa = 8.44ALKK159 pKa = 9.77RR160 pKa = 11.84ARR162 pKa = 11.84RR163 pKa = 11.84LAKK166 pKa = 9.61RR167 pKa = 11.84RR168 pKa = 11.84RR169 pKa = 11.84ALRR172 pKa = 11.84RR173 pKa = 11.84RR174 pKa = 11.84AALKK178 pKa = 10.17RR179 pKa = 11.84RR180 pKa = 11.84AALRR184 pKa = 11.84RR185 pKa = 11.84RR186 pKa = 11.84ARR188 pKa = 11.84RR189 pKa = 11.84LSKK192 pKa = 10.41RR193 pKa = 11.84SSLAKK198 pKa = 8.56RR199 pKa = 11.84CRR201 pKa = 11.84RR202 pKa = 11.84FVRR205 pKa = 11.84RR206 pKa = 11.84ATKK209 pKa = 10.35KK210 pKa = 10.08AVKK213 pKa = 10.04KK214 pKa = 9.4ATKK217 pKa = 10.2KK218 pKa = 9.96LQKK221 pKa = 10.08KK222 pKa = 8.87LKK224 pKa = 9.36KK225 pKa = 9.78VKK227 pKa = 10.05KK228 pKa = 8.95AAKK231 pKa = 9.77KK232 pKa = 9.88AVKK235 pKa = 9.62KK236 pKa = 10.58AKK238 pKa = 10.15KK239 pKa = 8.87QVKK242 pKa = 8.93KK243 pKa = 10.6AKK245 pKa = 9.8KK246 pKa = 8.92AARR249 pKa = 11.84KK250 pKa = 9.17AKK252 pKa = 9.57RR253 pKa = 11.84AAKK256 pKa = 9.96RR257 pKa = 11.84RR258 pKa = 11.84VALRR262 pKa = 11.84RR263 pKa = 11.84RR264 pKa = 11.84RR265 pKa = 11.84APRR268 pKa = 11.84KK269 pKa = 8.52CGRR272 pKa = 11.84ARR274 pKa = 11.84RR275 pKa = 11.84STRR278 pKa = 11.84KK279 pKa = 8.3ATKK282 pKa = 9.91KK283 pKa = 9.7AAKK286 pKa = 9.82KK287 pKa = 8.79ATKK290 pKa = 9.91KK291 pKa = 9.66QLKK294 pKa = 9.4KK295 pKa = 10.3QLKK298 pKa = 9.58KK299 pKa = 10.55LQKK302 pKa = 9.94KK303 pKa = 9.27LKK305 pKa = 8.88KK306 pKa = 9.98AKK308 pKa = 10.14KK309 pKa = 9.4AVKK312 pKa = 9.59KK313 pKa = 10.54AKK315 pKa = 10.03KK316 pKa = 8.44QAKK319 pKa = 8.52KK320 pKa = 10.46AKK322 pKa = 9.77KK323 pKa = 9.24AAKK326 pKa = 9.22KK327 pKa = 10.02AKK329 pKa = 9.8KK330 pKa = 9.35AAKK333 pKa = 9.55KK334 pKa = 9.15RR335 pKa = 11.84AARR338 pKa = 11.84RR339 pKa = 11.84KK340 pKa = 9.49VVLKK344 pKa = 10.71KK345 pKa = 10.4KK346 pKa = 9.87AAKK349 pKa = 9.73KK350 pKa = 9.9LNKK353 pKa = 9.31IVKK356 pKa = 9.88KK357 pKa = 10.41LLRR360 pKa = 11.84LKK362 pKa = 10.58KK363 pKa = 10.48KK364 pKa = 9.95LGKK367 pKa = 10.08RR368 pKa = 11.84FNPKK372 pKa = 9.14KK373 pKa = 9.92HH374 pKa = 5.47VPKK377 pKa = 10.47KK378 pKa = 9.66VRR380 pKa = 11.84KK381 pKa = 9.13LLRR384 pKa = 11.84KK385 pKa = 9.97LKK387 pKa = 9.25IQKK390 pKa = 8.9RR391 pKa = 11.84CRR393 pKa = 11.84RR394 pKa = 11.84SRR396 pKa = 11.84RR397 pKa = 11.84ARR399 pKa = 11.84RR400 pKa = 11.84TRR402 pKa = 11.84RR403 pKa = 11.84CRR405 pKa = 11.84KK406 pKa = 9.63VAVKK410 pKa = 10.42LPKK413 pKa = 10.35NLLKK417 pKa = 10.8KK418 pKa = 10.27LNKK421 pKa = 8.73VQKK424 pKa = 10.72SNN426 pKa = 3.27

Molecular weight: 50.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13588

8

13596

7262538

66

5561

534.2

60.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.529 ± 0.016

1.592 ± 0.012

5.106 ± 0.012

6.775 ± 0.023

4.61 ± 0.015

4.414 ± 0.026

2.79 ± 0.014

6.363 ± 0.017

7.246 ± 0.024

9.264 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.449 ± 0.008

6.217 ± 0.016

4.082 ± 0.017

4.709 ± 0.016

4.001 ± 0.012

9.947 ± 0.029

6.347 ± 0.018

5.423 ± 0.015

0.813 ± 0.005

3.318 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski