Cyclophragma undans nucleopolyhedrovirus
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 147 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A288Q7F6|A0A288Q7F6_9ABAC Ie1 OS=Cyclophragma undans nucleopolyhedrovirus OX=1906244 PE=4 SV=1
MM1 pKa = 7.27 NLDD4 pKa = 3.41 LPYY7 pKa = 10.76 YY8 pKa = 10.51 RR9 pKa = 11.84 LGNHH13 pKa = 6.15 EE14 pKa = 4.38 KK15 pKa = 10.79 VEE17 pKa = 4.14 YY18 pKa = 10.29 IPLKK22 pKa = 10.59 LALNDD27 pKa = 5.06 DD28 pKa = 4.08 ISNEE32 pKa = 3.98 QQQQQHH38 pKa = 4.9 QKK40 pKa = 8.98 DD41 pKa = 3.99 TFVHH45 pKa = 6.88 DD46 pKa = 3.81 EE47 pKa = 4.03 SGANGGVIIYY57 pKa = 10.04 NAGAEE62 pKa = 4.06 PAMPVAFIILISLAAFVALFLLLYY86 pKa = 9.9 IIYY89 pKa = 9.24 YY90 pKa = 8.95 YY91 pKa = 11.04 VVLRR95 pKa = 11.84 EE96 pKa = 3.91 QQQQYY101 pKa = 11.23 SDD103 pKa = 4.25 DD104 pKa = 4.02 NFDD107 pKa = 3.75 IDD109 pKa = 4.65 IEE111 pKa = 4.21 PSSLIFNKK119 pKa = 9.69 FDD121 pKa = 3.13
Molecular weight: 13.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.986
IPC2_protein 4.215
IPC_protein 4.139
Toseland 3.948
ProMoST 4.215
Dawson 4.126
Bjellqvist 4.329
Wikipedia 4.05
Rodwell 3.973
Grimsley 3.872
Solomon 4.113
Lehninger 4.075
Nozaki 4.24
DTASelect 4.457
Thurlkill 3.999
EMBOSS 4.062
Sillero 4.266
Patrickios 0.693
IPC_peptide 4.113
IPC2_peptide 4.24
IPC2.peptide.svr19 4.172
Protein with the highest isoelectric point:
>tr|A0A288Q7J0|A0A288Q7J0_9ABAC Cg30 OS=Cyclophragma undans nucleopolyhedrovirus OX=1906244 PE=4 SV=1
MM1 pKa = 7.46 VYY3 pKa = 9.47 TRR5 pKa = 11.84 RR6 pKa = 11.84 LSSPSSYY13 pKa = 8.8 TRR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 PSYY20 pKa = 8.79 RR21 pKa = 11.84 RR22 pKa = 11.84 SRR24 pKa = 11.84 SMEE27 pKa = 3.33 YY28 pKa = 9.55 RR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 PGRR34 pKa = 11.84 PRR36 pKa = 11.84 SPGRR40 pKa = 11.84 PRR42 pKa = 11.84 SPGRR46 pKa = 11.84 ARR48 pKa = 11.84 SQNGRR53 pKa = 11.84 YY54 pKa = 9.23 KK55 pKa = 10.5 RR56 pKa = 11.84 RR57 pKa = 11.84 NRR59 pKa = 11.84 SSGGRR64 pKa = 11.84 RR65 pKa = 11.84 RR66 pKa = 11.84 SYY68 pKa = 9.2 RR69 pKa = 11.84 TRR71 pKa = 11.84 RR72 pKa = 11.84 YY73 pKa = 9.88
Molecular weight: 8.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.263
IPC2_protein 11.023
IPC_protein 12.164
Toseland 12.31
ProMoST 12.808
Dawson 12.31
Bjellqvist 12.31
Wikipedia 12.778
Rodwell 11.828
Grimsley 12.34
Solomon 12.808
Lehninger 12.705
Nozaki 12.296
DTASelect 12.31
Thurlkill 12.31
EMBOSS 12.808
Sillero 12.31
Patrickios 11.55
IPC_peptide 12.808
IPC2_peptide 11.798
IPC2.peptide.svr19 9.11
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
147
0
147
41972
43
1265
285.5
32.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.974 ± 0.232
2.404 ± 0.116
6.311 ± 0.148
5.051 ± 0.156
4.644 ± 0.123
3.764 ± 0.137
2.506 ± 0.095
6.452 ± 0.133
6.266 ± 0.26
9.128 ± 0.153
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.604 ± 0.08
7.364 ± 0.195
3.629 ± 0.189
3.676 ± 0.197
5.082 ± 0.186
6.402 ± 0.177
5.885 ± 0.155
6.516 ± 0.176
0.751 ± 0.055
4.501 ± 0.107
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here