Amino acid dipepetide frequency for Paenibacillus sp. Leaf72

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.518AlaAla: 10.518 ± 0.124	0.781AlaCys: 0.781 ± 0.022	4.582AlaAsp: 4.582 ± 0.047	6.142AlaGlu: 6.142 ± 0.076	3.6AlaPhe: 3.6 ± 0.047	7.178AlaGly: 7.178 ± 0.074	1.425AlaHis: 1.425 ± 0.025	5.821AlaIle: 5.821 ± 0.059	4.431AlaLys: 4.431 ± 0.048	8.735AlaLeu: 8.735 ± 0.064	2.376AlaMet: 2.376 ± 0.036	2.864AlaAsn: 2.864 ± 0.04	2.888AlaPro: 2.888 ± 0.047	2.953AlaGln: 2.953 ± 0.034	3.458AlaArg: 3.458 ± 0.043	5.932AlaSer: 5.932 ± 0.057	3.958AlaThr: 3.958 ± 0.054	6.592AlaVal: 6.592 ± 0.063	0.979AlaTrp: 0.979 ± 0.022	2.896AlaTyr: 2.896 ± 0.04	0.001AlaXaa: 0.001 ± 0.001
Cys	0.541CysAla: 0.541 ± 0.017	0.112CysCys: 0.112 ± 0.007	0.362CysAsp: 0.362 ± 0.013	0.454CysGlu: 0.454 ± 0.016	0.339CysPhe: 0.339 ± 0.013	0.7CysGly: 0.7 ± 0.019	0.187CysHis: 0.187 ± 0.011	0.469CysIle: 0.469 ± 0.017	0.323CysLys: 0.323 ± 0.013	0.756CysLeu: 0.756 ± 0.022	0.213CysMet: 0.213 ± 0.01	0.242CysAsn: 0.242 ± 0.011	0.319CysPro: 0.319 ± 0.012	0.205CysGln: 0.205 ± 0.011	0.395CysArg: 0.395 ± 0.013	0.588CysSer: 0.588 ± 0.018	0.387CysThr: 0.387 ± 0.012	0.431CysVal: 0.431 ± 0.015	0.098CysTrp: 0.098 ± 0.006	0.276CysTyr: 0.276 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	4.017AspAla: 4.017 ± 0.054	0.372AspCys: 0.372 ± 0.014	2.376AspAsp: 2.376 ± 0.035	3.779AspGlu: 3.779 ± 0.046	2.158AspPhe: 2.158 ± 0.033	4.057AspGly: 4.057 ± 0.054	1.064AspHis: 1.064 ± 0.025	3.452AspIle: 3.452 ± 0.045	2.765AspLys: 2.765 ± 0.044	4.552AspLeu: 4.552 ± 0.049	1.383AspMet: 1.383 ± 0.026	1.818AspAsn: 1.818 ± 0.029	2.101AspPro: 2.101 ± 0.034	1.821AspGln: 1.821 ± 0.029	2.469AspArg: 2.469 ± 0.035	2.933AspSer: 2.933 ± 0.041	2.483AspThr: 2.483 ± 0.038	3.363AspVal: 3.363 ± 0.043	0.87AspTrp: 0.87 ± 0.021	2.169AspTyr: 2.169 ± 0.031	0.0AspXaa: 0.0 ± 0.0
Glu	6.703GluAla: 6.703 ± 0.068	0.362GluCys: 0.362 ± 0.014	2.938GluAsp: 2.938 ± 0.041	5.163GluGlu: 5.163 ± 0.062	2.042GluPhe: 2.042 ± 0.03	4.386GluGly: 4.386 ± 0.048	1.605GluHis: 1.605 ± 0.034	3.996GluIle: 3.996 ± 0.054	3.779GluLys: 3.779 ± 0.051	7.822GluLeu: 7.822 ± 0.082	1.929GluMet: 1.929 ± 0.031	2.379GluAsn: 2.379 ± 0.038	2.311GluPro: 2.311 ± 0.038	4.542GluGln: 4.542 ± 0.059	4.033GluArg: 4.033 ± 0.059	3.383GluSer: 3.383 ± 0.044	3.196GluThr: 3.196 ± 0.044	4.151GluVal: 4.151 ± 0.05	0.977GluTrp: 0.977 ± 0.023	1.974GluTyr: 1.974 ± 0.035	0.001GluXaa: 0.001 ± 0.001
Phe	3.377PheAla: 3.377 ± 0.046	0.343PheCys: 0.343 ± 0.013	2.284PheAsp: 2.284 ± 0.035	2.561PheGlu: 2.561 ± 0.036	1.816PhePhe: 1.816 ± 0.038	3.118PheGly: 3.118 ± 0.041	0.898PheHis: 0.898 ± 0.02	3.084PheIle: 3.084 ± 0.05	2.123PheLys: 2.123 ± 0.036	3.732PheLeu: 3.732 ± 0.054	1.16PheMet: 1.16 ± 0.023	1.787PheAsn: 1.787 ± 0.028	1.57PhePro: 1.57 ± 0.028	1.481PheGln: 1.481 ± 0.029	1.921PheArg: 1.921 ± 0.032	2.922PheSer: 2.922 ± 0.04	2.472PheThr: 2.472 ± 0.035	2.904PheVal: 2.904 ± 0.045	0.534PheTrp: 0.534 ± 0.018	1.568PheTyr: 1.568 ± 0.029	0.002PheXaa: 0.002 ± 0.001
Gly	5.918GlyAla: 5.918 ± 0.067	0.681GlyCys: 0.681 ± 0.021	3.436GlyAsp: 3.436 ± 0.048	4.776GlyGlu: 4.776 ± 0.05	3.188GlyPhe: 3.188 ± 0.041	5.557GlyGly: 5.557 ± 0.084	1.46GlyHis: 1.46 ± 0.023	5.395GlyIle: 5.395 ± 0.057	4.528GlyLys: 4.528 ± 0.056	7.092GlyLeu: 7.092 ± 0.07	2.297GlyMet: 2.297 ± 0.037	2.732GlyAsn: 2.732 ± 0.054	1.836GlyPro: 1.836 ± 0.034	2.77GlyGln: 2.77 ± 0.042	3.221GlyArg: 3.221 ± 0.052	5.031GlySer: 5.031 ± 0.062	4.382GlyThr: 4.382 ± 0.064	4.892GlyVal: 4.892 ± 0.056	1.092GlyTrp: 1.092 ± 0.026	2.878GlyTyr: 2.878 ± 0.04	0.002GlyXaa: 0.002 ± 0.001
His	1.701HisAla: 1.701 ± 0.028	0.189HisCys: 0.189 ± 0.01	0.958HisAsp: 0.958 ± 0.022	1.294HisGlu: 1.294 ± 0.026	1.087HisPhe: 1.087 ± 0.027	1.402HisGly: 1.402 ± 0.026	0.603HisHis: 0.603 ± 0.02	1.484HisIle: 1.484 ± 0.029	0.872HisLys: 0.872 ± 0.021	2.082HisLeu: 2.082 ± 0.039	0.593HisMet: 0.593 ± 0.015	0.714HisAsn: 0.714 ± 0.02	1.101HisPro: 1.101 ± 0.026	0.777HisGln: 0.777 ± 0.02	0.952HisArg: 0.952 ± 0.025	1.231HisSer: 1.231 ± 0.024	1.091HisThr: 1.091 ± 0.024	1.317HisVal: 1.317 ± 0.026	0.306HisTrp: 0.306 ± 0.013	0.96HisTyr: 0.96 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	6.228IleAla: 6.228 ± 0.058	0.586IleCys: 0.586 ± 0.018	3.648IleAsp: 3.648 ± 0.041	4.302IleGlu: 4.302 ± 0.059	2.488IlePhe: 2.488 ± 0.047	5.266IleGly: 5.266 ± 0.054	1.431IleHis: 1.431 ± 0.029	4.568IleIle: 4.568 ± 0.064	3.19IleLys: 3.19 ± 0.045	5.202IleLeu: 5.202 ± 0.062	1.714IleMet: 1.714 ± 0.03	2.558IleAsn: 2.558 ± 0.036	2.935IlePro: 2.935 ± 0.043	2.508IleGln: 2.508 ± 0.037	3.541IleArg: 3.541 ± 0.046	4.519IleSer: 4.519 ± 0.05	3.775IleThr: 3.775 ± 0.048	5.219IleVal: 5.219 ± 0.06	0.763IleTrp: 0.763 ± 0.02	2.106IleTyr: 2.106 ± 0.032	0.001IleXaa: 0.001 ± 0.001
Lys	4.544LysAla: 4.544 ± 0.051	0.247LysCys: 0.247 ± 0.011	2.625LysAsp: 2.625 ± 0.041	4.108LysGlu: 4.108 ± 0.055	1.582LysPhe: 1.582 ± 0.029	3.467LysGly: 3.467 ± 0.041	1.202LysHis: 1.202 ± 0.029	3.136LysIle: 3.136 ± 0.04	3.085LysLys: 3.085 ± 0.048	5.981LysLeu: 5.981 ± 0.065	1.632LysMet: 1.632 ± 0.029	2.102LysAsn: 2.102 ± 0.036	2.237LysPro: 2.237 ± 0.034	2.92LysGln: 2.92 ± 0.04	2.799LysArg: 2.799 ± 0.04	2.881LysSer: 2.881 ± 0.036	2.746LysThr: 2.746 ± 0.046	3.455LysVal: 3.455 ± 0.044	0.745LysTrp: 0.745 ± 0.017	1.735LysTyr: 1.735 ± 0.033	0.001LysXaa: 0.001 ± 0.001
Leu	9.223LeuAla: 9.223 ± 0.078	0.81LeuCys: 0.81 ± 0.021	5.082LeuAsp: 5.082 ± 0.049	6.452LeuGlu: 6.452 ± 0.069	4.708LeuPhe: 4.708 ± 0.071	6.76LeuGly: 6.76 ± 0.058	2.165LeuHis: 2.165 ± 0.033	6.403LeuIle: 6.403 ± 0.069	5.301LeuLys: 5.301 ± 0.067	11.833LeuLeu: 11.833 ± 0.12	2.654LeuMet: 2.654 ± 0.034	3.933LeuAsn: 3.933 ± 0.043	4.563LeuPro: 4.563 ± 0.05	4.187LeuGln: 4.187 ± 0.055	4.831LeuArg: 4.831 ± 0.053	7.182LeuSer: 7.182 ± 0.065	5.634LeuThr: 5.634 ± 0.051	6.198LeuVal: 6.198 ± 0.059	1.082LeuTrp: 1.082 ± 0.025	3.322LeuTyr: 3.322 ± 0.045	0.002LeuXaa: 0.002 ± 0.001
Met	2.292MetAla: 2.292 ± 0.036	0.146MetCys: 0.146 ± 0.008	1.516MetAsp: 1.516 ± 0.027	1.876MetGlu: 1.876 ± 0.034	1.016MetPhe: 1.016 ± 0.024	1.642MetGly: 1.642 ± 0.036	0.473MetHis: 0.473 ± 0.014	1.815MetIle: 1.815 ± 0.034	2.028MetLys: 2.028 ± 0.029	3.261MetLeu: 3.261 ± 0.042	0.858MetMet: 0.858 ± 0.024	1.475MetAsn: 1.475 ± 0.029	1.158MetPro: 1.158 ± 0.022	1.103MetGln: 1.103 ± 0.025	1.285MetArg: 1.285 ± 0.023	1.798MetSer: 1.798 ± 0.032	1.667MetThr: 1.667 ± 0.03	1.658MetVal: 1.658 ± 0.027	0.248MetTrp: 0.248 ± 0.01	0.793MetTyr: 0.793 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.019AsnAla: 3.019 ± 0.046	0.258AsnCys: 0.258 ± 0.011	2.019AsnAsp: 2.019 ± 0.027	2.71AsnGlu: 2.71 ± 0.041	1.318AsnPhe: 1.318 ± 0.028	3.401AsnGly: 3.401 ± 0.061	0.83AsnHis: 0.83 ± 0.019	2.336AsnIle: 2.336 ± 0.035	2.159AsnLys: 2.159 ± 0.031	3.133AsnLeu: 3.133 ± 0.034	1.042AsnMet: 1.042 ± 0.021	1.694AsnAsn: 1.694 ± 0.038	1.976AsnPro: 1.976 ± 0.035	1.564AsnGln: 1.564 ± 0.029	2.054AsnArg: 2.054 ± 0.038	2.233AsnSer: 2.233 ± 0.038	2.087AsnThr: 2.087 ± 0.034	2.607AsnVal: 2.607 ± 0.036	0.555AsnTrp: 0.555 ± 0.015	1.447AsnTyr: 1.447 ± 0.028	0.001AsnXaa: 0.001 ± 0.001
Pro	3.484ProAla: 3.484 ± 0.047	0.217ProCys: 0.217 ± 0.01	2.354ProAsp: 2.354 ± 0.032	3.142ProGlu: 3.142 ± 0.043	1.961ProPhe: 1.961 ± 0.031	2.707ProGly: 2.707 ± 0.039	0.883ProHis: 0.883 ± 0.022	2.593ProIle: 2.593 ± 0.039	1.68ProLys: 1.68 ± 0.03	4.059ProLeu: 4.059 ± 0.053	0.936ProMet: 0.936 ± 0.02	1.53ProAsn: 1.53 ± 0.03	1.165ProPro: 1.165 ± 0.029	1.503ProGln: 1.503 ± 0.025	1.29ProArg: 1.29 ± 0.026	2.601ProSer: 2.601 ± 0.04	1.992ProThr: 1.992 ± 0.045	2.935ProVal: 2.935 ± 0.041	0.472ProTrp: 0.472 ± 0.015	1.533ProTyr: 1.533 ± 0.028	0.001ProXaa: 0.001 ± 0.001
Gln	3.941GlnAla: 3.941 ± 0.05	0.193GlnCys: 0.193 ± 0.009	1.623GlnAsp: 1.623 ± 0.029	2.705GlnGlu: 2.705 ± 0.041	1.615GlnPhe: 1.615 ± 0.029	2.667GlnGly: 2.667 ± 0.038	0.925GlnHis: 0.925 ± 0.024	2.551GlnIle: 2.551 ± 0.036	1.898GlnLys: 1.898 ± 0.027	5.094GlnLeu: 5.094 ± 0.06	1.254GlnMet: 1.254 ± 0.025	1.432GlnAsn: 1.432 ± 0.024	1.655GlnPro: 1.655 ± 0.027	2.48GlnGln: 2.48 ± 0.046	1.954GlnArg: 1.954 ± 0.029	2.47GlnSer: 2.47 ± 0.032	2.153GlnThr: 2.153 ± 0.032	2.538GlnVal: 2.538 ± 0.038	0.56GlnTrp: 0.56 ± 0.018	1.449GlnTyr: 1.449 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	3.272ArgAla: 3.272 ± 0.04	0.329ArgCys: 0.329 ± 0.013	2.187ArgAsp: 2.187 ± 0.037	3.462ArgGlu: 3.462 ± 0.051	2.196ArgPhe: 2.196 ± 0.031	2.824ArgGly: 2.824 ± 0.037	0.976ArgHis: 0.976 ± 0.022	3.385ArgIle: 3.385 ± 0.048	2.955ArgLys: 2.955 ± 0.048	5.255ArgLeu: 5.255 ± 0.056	1.57ArgMet: 1.57 ± 0.031	1.83ArgAsn: 1.83 ± 0.029	1.572ArgPro: 1.572 ± 0.031	2.044ArgGln: 2.044 ± 0.037	2.405ArgArg: 2.405 ± 0.041	3.148ArgSer: 3.148 ± 0.035	2.51ArgThr: 2.51 ± 0.039	2.891ArgVal: 2.891 ± 0.044	0.606ArgTrp: 0.606 ± 0.017	1.911ArgTyr: 1.911 ± 0.034	0.002ArgXaa: 0.002 ± 0.001
Ser	5.227SerAla: 5.227 ± 0.062	0.481SerCys: 0.481 ± 0.016	3.134SerAsp: 3.134 ± 0.045	4.063SerGlu: 4.063 ± 0.045	3.158SerPhe: 3.158 ± 0.043	5.645SerGly: 5.645 ± 0.07	1.241SerHis: 1.241 ± 0.025	4.46SerIle: 4.46 ± 0.047	3.349SerLys: 3.349 ± 0.041	6.488SerLeu: 6.488 ± 0.062	1.791SerMet: 1.791 ± 0.033	2.394SerAsn: 2.394 ± 0.042	2.528SerPro: 2.528 ± 0.038	2.176SerGln: 2.176 ± 0.031	2.978SerArg: 2.978 ± 0.041	4.846SerSer: 4.846 ± 0.077	3.274SerThr: 3.274 ± 0.051	4.394SerVal: 4.394 ± 0.055	0.882SerTrp: 0.882 ± 0.022	2.51SerTyr: 2.51 ± 0.043	0.002SerXaa: 0.002 ± 0.001
Thr	5.101ThrAla: 5.101 ± 0.058	0.314ThrCys: 0.314 ± 0.014	2.881ThrAsp: 2.881 ± 0.044	3.218ThrGlu: 3.218 ± 0.045	2.307ThrPhe: 2.307 ± 0.036	4.435ThrGly: 4.435 ± 0.061	0.954ThrHis: 0.954 ± 0.025	3.938ThrIle: 3.938 ± 0.052	2.469ThrLys: 2.469 ± 0.035	5.395ThrLeu: 5.395 ± 0.055	1.327ThrMet: 1.327 ± 0.021	2.101ThrAsn: 2.101 ± 0.039	2.46ThrPro: 2.46 ± 0.042	1.594ThrGln: 1.594 ± 0.028	2.056ThrArg: 2.056 ± 0.029	3.349ThrSer: 3.349 ± 0.047	2.969ThrThr: 2.969 ± 0.047	4.366ThrVal: 4.366 ± 0.055	0.584ThrTrp: 0.584 ± 0.018	1.87ThrTyr: 1.87 ± 0.04	0.001ThrXaa: 0.001 ± 0.001
Val	5.284ValAla: 5.284 ± 0.049	0.576ValCys: 0.576 ± 0.018	3.439ValAsp: 3.439 ± 0.042	4.257ValGlu: 4.257 ± 0.045	2.845ValPhe: 2.845 ± 0.044	4.411ValGly: 4.411 ± 0.052	1.395ValHis: 1.395 ± 0.024	4.718ValIle: 4.718 ± 0.059	3.826ValLys: 3.826 ± 0.047	6.975ValLeu: 6.975 ± 0.062	1.912ValMet: 1.912 ± 0.029	2.706ValAsn: 2.706 ± 0.04	2.772ValPro: 2.772 ± 0.043	2.698ValGln: 2.698 ± 0.04	3.121ValArg: 3.121 ± 0.039	4.724ValSer: 4.724 ± 0.046	4.261ValThr: 4.261 ± 0.068	4.738ValVal: 4.738 ± 0.056	0.837ValTrp: 0.837 ± 0.024	2.371ValTyr: 2.371 ± 0.037	0.001ValXaa: 0.001 ± 0.001
Trp	0.845TrpAla: 0.845 ± 0.022	0.097TrpCys: 0.097 ± 0.008	0.638TrpAsp: 0.638 ± 0.019	0.806TrpGlu: 0.806 ± 0.021	0.574TrpPhe: 0.574 ± 0.02	0.857TrpGly: 0.857 ± 0.022	0.304TrpHis: 0.304 ± 0.013	0.83TrpIle: 0.83 ± 0.023	0.722TrpLys: 0.722 ± 0.02	1.511TrpLeu: 1.511 ± 0.029	0.423TrpMet: 0.423 ± 0.014	0.66TrpAsn: 0.66 ± 0.017	0.388TrpPro: 0.388 ± 0.013	0.562TrpGln: 0.562 ± 0.018	0.616TrpArg: 0.616 ± 0.016	0.916TrpSer: 0.916 ± 0.025	0.721TrpThr: 0.721 ± 0.022	0.773TrpVal: 0.773 ± 0.017	0.173TrpTrp: 0.173 ± 0.009	0.441TrpTyr: 0.441 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.873TyrAla: 2.873 ± 0.043	0.3TyrCys: 0.3 ± 0.012	1.923TyrAsp: 1.923 ± 0.053	2.402TyrGlu: 2.402 ± 0.037	1.661TyrPhe: 1.661 ± 0.031	2.683TyrGly: 2.683 ± 0.04	0.695TyrHis: 0.695 ± 0.017	2.113TyrIle: 2.113 ± 0.029	1.747TyrLys: 1.747 ± 0.028	3.365TyrLeu: 3.365 ± 0.043	1.045TyrMet: 1.045 ± 0.02	1.49TyrAsn: 1.49 ± 0.029	1.499TyrPro: 1.499 ± 0.029	1.266TyrGln: 1.266 ± 0.026	1.947TyrArg: 1.947 ± 0.031	2.331TyrSer: 2.331 ± 0.035	1.932TyrThr: 1.932 ± 0.04	2.442TyrVal: 2.442 ± 0.032	0.495TyrTrp: 0.495 ± 0.013	1.438TyrTyr: 1.438 ± 0.028	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.003XaaGly: 0.003 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.001XaaAsn: 0.001 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.002XaaSer: 0.002 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 6399 proteins (2116404 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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