Mosquito VEM Anellovirus SDBVL B

Taxonomy: Viruses; Anelloviridae; unclassified Anelloviridae

Average proteome isoelectric point is 7.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6KID2|F6KID2_9VIRU Capsid protein OS=Mosquito VEM Anellovirus SDBVL B OX=1034791 PE=3 SV=1
MM1 pKa = 7.75AFTASLGQDD10 pKa = 3.09EE11 pKa = 5.51DD12 pKa = 3.11RR13 pKa = 11.84WKK15 pKa = 11.13LVIQTSHH22 pKa = 6.51MLFCDD27 pKa = 3.24CGDD30 pKa = 3.92YY31 pKa = 11.01VKK33 pKa = 10.6HH34 pKa = 5.89LQNILGPQWLCTSEE48 pKa = 4.76EE49 pKa = 4.07EE50 pKa = 5.34GGTGDD55 pKa = 3.89QSGAGEE61 pKa = 4.25ATGEE65 pKa = 4.22DD66 pKa = 3.86PTDD69 pKa = 3.52QLMAAALDD77 pKa = 3.9AAEE80 pKa = 4.68EE81 pKa = 4.13LAGAPGSGG89 pKa = 3.22

Molecular weight: 9.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F6KID3|F6KID3_9VIRU Uncharacterized protein OS=Mosquito VEM Anellovirus SDBVL B OX=1034791 PE=4 SV=1
MM1 pKa = 7.54ALYY4 pKa = 9.76FRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84YY11 pKa = 8.08WRR13 pKa = 11.84PKK15 pKa = 6.8WRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84GYY21 pKa = 8.14WRR23 pKa = 11.84RR24 pKa = 11.84SYY26 pKa = 10.7RR27 pKa = 11.84PAYY30 pKa = 9.45GRR32 pKa = 11.84RR33 pKa = 11.84LRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84TRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84SRR45 pKa = 11.84VRR47 pKa = 11.84VRR49 pKa = 11.84VLTEE53 pKa = 3.47RR54 pKa = 11.84QPRR57 pKa = 11.84YY58 pKa = 8.33WRR60 pKa = 11.84RR61 pKa = 11.84CTIRR65 pKa = 11.84GWWPVLITGRR75 pKa = 11.84KK76 pKa = 7.69YY77 pKa = 10.69PSTAAAGDD85 pKa = 3.76DD86 pKa = 4.37RR87 pKa = 11.84GTQMLTFKK95 pKa = 10.27PFQPFGSKK103 pKa = 10.44LSNAWFYY110 pKa = 11.34RR111 pKa = 11.84DD112 pKa = 3.59YY113 pKa = 11.05EE114 pKa = 4.16IVRR117 pKa = 11.84GGFSAGTFSLQALYY131 pKa = 9.61MEE133 pKa = 5.38HH134 pKa = 6.84LMCRR138 pKa = 11.84NRR140 pKa = 11.84WSQSNCGFDD149 pKa = 3.28LASYY153 pKa = 10.31RR154 pKa = 11.84GTKK157 pKa = 10.09LYY159 pKa = 9.93FVPHH163 pKa = 6.85PNYY166 pKa = 10.77DD167 pKa = 4.12YY168 pKa = 11.1IVLIDD173 pKa = 3.4PEE175 pKa = 4.31YY176 pKa = 11.01RR177 pKa = 11.84KK178 pKa = 10.14FEE180 pKa = 3.91QWIKK184 pKa = 10.76QPMHH188 pKa = 6.55PGVLITHH195 pKa = 6.17PQSRR199 pKa = 11.84IIRR202 pKa = 11.84SITHH206 pKa = 6.37AGPRR210 pKa = 11.84RR211 pKa = 11.84KK212 pKa = 8.68MPKK215 pKa = 8.94MFVPPPSTMNSGWQWMGEE233 pKa = 4.03LANEE237 pKa = 4.3GLFAFYY243 pKa = 10.2VQWIDD248 pKa = 5.72LGAPWLGDD256 pKa = 3.3VQNPNQVKK264 pKa = 8.2WWKK267 pKa = 9.23TGDD270 pKa = 3.42ATTKK274 pKa = 10.19PDD276 pKa = 3.25WVTKK280 pKa = 9.9GIEE283 pKa = 4.16MQGKK287 pKa = 6.7TVQEE291 pKa = 4.21GLDD294 pKa = 3.26AYY296 pKa = 8.81YY297 pKa = 9.31TQAGQGGKK305 pKa = 9.51KK306 pKa = 8.22WVNPGGAPYY315 pKa = 10.5QLGFGPFVFKK325 pKa = 10.71GSHH328 pKa = 6.15SGDD331 pKa = 3.41QEE333 pKa = 4.54HH334 pKa = 6.55YY335 pKa = 9.54PQICFFYY342 pKa = 10.47KK343 pKa = 10.46SYY345 pKa = 8.74WQWGGSTTSIKK356 pKa = 10.46NVCDD360 pKa = 3.8PKK362 pKa = 11.2DD363 pKa = 3.79NPNAEE368 pKa = 4.44YY369 pKa = 10.78LSAWNRR375 pKa = 11.84AGFQPAQARR384 pKa = 11.84NNDD387 pKa = 3.88IYY389 pKa = 11.22NQPP392 pKa = 3.25

Molecular weight: 46.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

749

89

392

249.7

28.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.142 ± 1.274

1.602 ± 0.376

4.406 ± 0.937

4.94 ± 1.557

3.605 ± 0.897

8.411 ± 1.33

2.27 ± 0.38

3.738 ± 0.31

4.806 ± 0.522

6.409 ± 1.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.537 ± 0.189

3.338 ± 0.598

6.943 ± 0.73

5.607 ± 0.378

9.613 ± 1.813

7.61 ± 2.165

6.943 ± 1.454

3.872 ± 0.4

3.204 ± 1.413

4.005 ± 1.524
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski