Nocardiopsis sp. JB363

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Nocardiopsaceae; Nocardiopsis; unclassified Nocardiopsis

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6273 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1R3ULY7|A0A1R3ULY7_9ACTN Uncharacterized protein SCO1141 OS=Nocardiopsis sp. JB363 OX=1434837 GN=BQ8420_14230 PE=3 SV=1
MM1 pKa = 7.49HH2 pKa = 7.37RR3 pKa = 11.84TTLTAGCAVLLLSLAACSGGDD24 pKa = 3.7GEE26 pKa = 4.95SEE28 pKa = 4.07EE29 pKa = 5.46DD30 pKa = 4.06GLSADD35 pKa = 3.67IDD37 pKa = 3.94AAPVRR42 pKa = 11.84AFGPGCADD50 pKa = 4.31FPDD53 pKa = 4.36TGPGSLSTMAGQPLSVAVADD73 pKa = 4.11NPLLSRR79 pKa = 11.84LSTLLDD85 pKa = 3.58RR86 pKa = 11.84SGLGPDD92 pKa = 4.68LDD94 pKa = 4.41SADD97 pKa = 4.75DD98 pKa = 3.71MTLFAPTDD106 pKa = 3.8DD107 pKa = 4.56AFDD110 pKa = 3.69MYY112 pKa = 10.36PAEE115 pKa = 4.77EE116 pKa = 4.33IQDD119 pKa = 4.34LMDD122 pKa = 4.81APDD125 pKa = 4.54GPAHH129 pKa = 6.84LLGHH133 pKa = 5.95HH134 pKa = 5.89VVLGEE139 pKa = 4.12FGSAEE144 pKa = 4.06LDD146 pKa = 3.32GGSFDD151 pKa = 3.84TLNGDD156 pKa = 3.36RR157 pKa = 11.84VGTTEE162 pKa = 4.44SEE164 pKa = 4.14GEE166 pKa = 4.25YY167 pKa = 9.8TVEE170 pKa = 4.54GYY172 pKa = 11.3APVVCADD179 pKa = 3.26VHH181 pKa = 5.26TANATVHH188 pKa = 4.81VVGMLLIPSS197 pKa = 4.12

Molecular weight: 20.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1R3UV54|A0A1R3UV54_9ACTN Deoxyguanosinetriphosphate triphosphohydrolase OS=Nocardiopsis sp. JB363 OX=1434837 GN=BQ8420_28450 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIASRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.5GRR40 pKa = 11.84AALTVAHH47 pKa = 7.09

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6273

0

6273

1999419

37

6538

318.7

34.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.85 ± 0.041

0.705 ± 0.009

6.412 ± 0.03

6.82 ± 0.031

2.862 ± 0.017

9.212 ± 0.031

2.474 ± 0.019

3.429 ± 0.019

1.747 ± 0.022

10.379 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.026 ± 0.012

1.86 ± 0.015

5.916 ± 0.023

2.578 ± 0.02

8.071 ± 0.04

5.383 ± 0.023

6.079 ± 0.019

8.704 ± 0.034

1.525 ± 0.013

1.968 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski