Helicobacter phage PtB92G

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Schmidvirus; unclassified Schmidvirus

Average proteome isoelectric point is 7.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 39 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5RG95|A0A1S5RG95_9CAUD Putative tail assembly protein OS=Helicobacter phage PtB92G OX=1852682 PE=4 SV=1
MM1 pKa = 7.66GIKK4 pKa = 9.82EE5 pKa = 4.23KK6 pKa = 10.55EE7 pKa = 3.99IEE9 pKa = 4.18LEE11 pKa = 3.99TLKK14 pKa = 11.01RR15 pKa = 11.84EE16 pKa = 3.99IAQAEE21 pKa = 4.06ASLEE25 pKa = 3.89QDD27 pKa = 4.45FIKK30 pKa = 10.94HH31 pKa = 4.8MVEE34 pKa = 3.69KK35 pKa = 10.0TNEE38 pKa = 3.95KK39 pKa = 10.56VEE41 pKa = 4.87DD42 pKa = 3.83LFFSNKK48 pKa = 8.85PEE50 pKa = 4.27FYY52 pKa = 10.1RR53 pKa = 11.84FVFTEE58 pKa = 3.6QNNYY62 pKa = 9.57LRR64 pKa = 11.84EE65 pKa = 4.0KK66 pKa = 10.13LTDD69 pKa = 3.4KK70 pKa = 10.8VGRR73 pKa = 11.84AIDD76 pKa = 4.06LSDD79 pKa = 4.16EE80 pKa = 4.12IQRR83 pKa = 11.84DD84 pKa = 3.65RR85 pKa = 11.84EE86 pKa = 4.0NEE88 pKa = 3.96EE89 pKa = 3.62IEE91 pKa = 4.04KK92 pKa = 10.98DD93 pKa = 3.25KK94 pKa = 11.4EE95 pKa = 4.14AFLKK99 pKa = 10.25KK100 pKa = 10.1HH101 pKa = 6.07PNIDD105 pKa = 3.86LNEE108 pKa = 4.16LLDD111 pKa = 4.19FYY113 pKa = 11.61NEE115 pKa = 4.19EE116 pKa = 3.62IPNRR120 pKa = 11.84FKK122 pKa = 11.17KK123 pKa = 10.58QIDD126 pKa = 3.72KK127 pKa = 11.52LEE129 pKa = 4.31GEE131 pKa = 4.73AFFEE135 pKa = 5.78AILDD139 pKa = 3.77YY140 pKa = 11.03FNAVIAKK147 pKa = 9.89EE148 pKa = 3.99EE149 pKa = 4.08EE150 pKa = 4.15PKK152 pKa = 10.84NEE154 pKa = 4.18EE155 pKa = 3.94KK156 pKa = 10.87EE157 pKa = 4.31EE158 pKa = 3.96EE159 pKa = 4.22SKK161 pKa = 11.13LPKK164 pKa = 9.77EE165 pKa = 3.94ALGNGVSGVGYY176 pKa = 10.54ANNGNIMTRR185 pKa = 11.84YY186 pKa = 9.44

Molecular weight: 21.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5RG62|A0A1S5RG62_9CAUD Uncharacterized protein OS=Helicobacter phage PtB92G OX=1852682 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 9.94PKK4 pKa = 10.65KK5 pKa = 10.04NLNKK9 pKa = 9.94PLRR12 pKa = 11.84KK13 pKa = 9.61KK14 pKa = 10.61ADD16 pKa = 3.58RR17 pKa = 11.84FEE19 pKa = 4.01WALIIAFSLILATTYY34 pKa = 8.73TLRR37 pKa = 11.84SLLL40 pKa = 3.89

Molecular weight: 4.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

39

0

39

9946

40

1584

255.0

29.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.515 ± 0.399

0.865 ± 0.199

4.645 ± 0.356

8.526 ± 0.352

5.027 ± 0.235

4.062 ± 0.271

1.538 ± 0.214

6.937 ± 0.264

10.889 ± 0.38

10.758 ± 0.342

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.719 ± 0.178

7.983 ± 0.417

2.514 ± 0.154

4.635 ± 0.332

3.861 ± 0.22

6.616 ± 0.247

4.746 ± 0.225

4.193 ± 0.316

0.483 ± 0.086

3.489 ± 0.218
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski