Bacteriophage vB_NpeS-2AV2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ravarandavirus; Nodularia virus 2AV2

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 182 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L2BWU0|A0A1L2BWU0_9CAUD Uncharacterized protein OS=Bacteriophage vB_NpeS-2AV2 OX=1777122 GN=2AV2_57 PE=4 SV=1
MM1 pKa = 7.09SQLKK5 pKa = 9.91IVEE8 pKa = 4.54DD9 pKa = 3.63NLFVFVLNFSDD20 pKa = 3.91TVVDD24 pKa = 4.82LYY26 pKa = 11.39EE27 pKa = 3.9VLKK30 pKa = 10.99VYY32 pKa = 10.38NQYY35 pKa = 11.71AFLSDD40 pKa = 5.08DD41 pKa = 4.54FFPDD45 pKa = 2.9IYY47 pKa = 11.46EE48 pKa = 4.14NLCVNAYY55 pKa = 8.31NARR58 pKa = 11.84NMEE61 pKa = 4.46DD62 pKa = 3.33LRR64 pKa = 11.84II65 pKa = 3.93

Molecular weight: 7.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L2BWT1|A0A1L2BWT1_9CAUD Uncharacterized protein OS=Bacteriophage vB_NpeS-2AV2 OX=1777122 GN=2AV2_42 PE=4 SV=1
MM1 pKa = 7.47IIVRR5 pKa = 11.84GDD7 pKa = 3.12RR8 pKa = 11.84FRR10 pKa = 11.84DD11 pKa = 3.5VRR13 pKa = 11.84VCCLLLIGAKK23 pKa = 9.45KK24 pKa = 8.21WWVVTGNSKK33 pKa = 10.25LLKK36 pKa = 9.83ICSTNVWTTRR46 pKa = 11.84AKK48 pKa = 10.78NKK50 pKa = 10.44LKK52 pKa = 10.63GRR54 pKa = 11.84SPWVYY59 pKa = 10.92

Molecular weight: 6.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

182

0

182

41335

15

3404

227.1

25.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.251 ± 0.276

1.176 ± 0.147

6.101 ± 0.137

6.759 ± 0.242

3.873 ± 0.191

6.063 ± 0.217

1.507 ± 0.145

6.176 ± 0.186

7.338 ± 0.342

9.263 ± 0.21

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.805 ± 0.144

4.981 ± 0.148

4.151 ± 0.166

4.679 ± 0.325

4.65 ± 0.148

7.03 ± 0.223

6.249 ± 0.224

6.363 ± 0.151

1.103 ± 0.107

3.481 ± 0.204
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski