Candidatus Paraburkholderia sp. 4M-K11

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia; unclassified Paraburkholderia

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7801 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R5MF20|A0A4R5MF20_9BURK Uncharacterized protein OS=Candidatus Paraburkholderia sp. 4M-K11 OX=2528715 GN=EYW47_00260 PE=4 SV=1
MM1 pKa = 7.81VYY3 pKa = 10.42DD4 pKa = 4.36SSAHH8 pKa = 6.03DD9 pKa = 3.63TLTLGGADD17 pKa = 3.19ASAPVALHH25 pKa = 5.9NVANGDD31 pKa = 3.78LSVASTDD38 pKa = 3.5AVNGSQLYY46 pKa = 8.13ATNSNISNLSGDD58 pKa = 3.61VTNIQGDD65 pKa = 3.52ITNINGKK72 pKa = 9.18LADD75 pKa = 3.68AVIYY79 pKa = 10.34DD80 pKa = 4.07SSAHH84 pKa = 5.92NSVTLGGAGASVPVALHH101 pKa = 5.64NVANGDD107 pKa = 3.78LSVASTDD114 pKa = 3.5AVNGSQLFATNSNISNLSGDD134 pKa = 3.61VTNIQGDD141 pKa = 3.52ITNINGKK148 pKa = 9.17LADD151 pKa = 3.6AVVYY155 pKa = 10.5DD156 pKa = 4.35SSAHH160 pKa = 5.69NSVTLGGAGASVPVALHH177 pKa = 5.64NVANGDD183 pKa = 3.78LSVASTDD190 pKa = 3.6AVNGAQLFATNSNISNLSGDD210 pKa = 3.58VTNIQQ215 pKa = 3.31

Molecular weight: 21.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R5MCS6|A0A4R5MCS6_9BURK DUF2968 domain-containing protein OS=Candidatus Paraburkholderia sp. 4M-K11 OX=2528715 GN=EYW47_09510 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.74TAGGRR28 pKa = 11.84KK29 pKa = 9.04VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LAII44 pKa = 4.0

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7801

0

7801

2532828

27

3316

324.7

35.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.213 ± 0.047

0.978 ± 0.009

5.268 ± 0.021

5.219 ± 0.028

3.646 ± 0.018

8.316 ± 0.033

2.357 ± 0.012

4.551 ± 0.018

2.954 ± 0.021

10.089 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.342 ± 0.012

2.837 ± 0.021

5.098 ± 0.02

3.669 ± 0.015

6.931 ± 0.03

5.643 ± 0.027

5.362 ± 0.023

7.66 ± 0.021

1.393 ± 0.011

2.473 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski