Canis familiaris papillomavirus 7
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C8YJK0|C8YJK0_9PAPI Replication protein E1 OS=Canis familiaris papillomavirus 7 OX=2759772 GN=E1 PE=3 SV=1
MM1 pKa = 7.75 RR2 pKa = 11.84 GSEE5 pKa = 4.07 SDD7 pKa = 3.33 VRR9 pKa = 11.84 DD10 pKa = 3.33 IVLDD14 pKa = 3.4 LHH16 pKa = 6.79 EE17 pKa = 5.51 LVLPQNLLSSEE28 pKa = 4.11 VLEE31 pKa = 4.54 TEE33 pKa = 4.15 EE34 pKa = 4.82 EE35 pKa = 4.31 EE36 pKa = 4.57 PEE38 pKa = 4.32 PEE40 pKa = 3.92 PGRR43 pKa = 11.84 YY44 pKa = 8.06 RR45 pKa = 11.84 VVTCCNLCHH54 pKa = 6.64 SPLRR58 pKa = 11.84 LFIEE62 pKa = 4.68 SADD65 pKa = 3.71 EE66 pKa = 4.14 GQVRR70 pKa = 11.84 IFHH73 pKa = 6.04 QLLLDD78 pKa = 3.91 GLGILCAVCYY88 pKa = 7.75 RR89 pKa = 11.84 THH91 pKa = 6.59 CCDD94 pKa = 2.63 GRR96 pKa = 11.84 RR97 pKa = 11.84 RR98 pKa = 3.61
Molecular weight: 11.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.708
IPC2_protein 4.876
IPC_protein 4.749
Toseland 4.66
ProMoST 4.863
Dawson 4.711
Bjellqvist 4.851
Wikipedia 4.558
Rodwell 4.635
Grimsley 4.571
Solomon 4.711
Lehninger 4.66
Nozaki 4.825
DTASelect 4.927
Thurlkill 4.66
EMBOSS 4.584
Sillero 4.902
Patrickios 3.274
IPC_peptide 4.724
IPC2_peptide 4.902
IPC2.peptide.svr19 4.84
Protein with the highest isoelectric point:
>tr|C8YJK2|C8YJK2_9PAPI E4 (Fragment) OS=Canis familiaris papillomavirus 7 OX=2759772 PE=4 SV=1
MM1 pKa = 7.22 MEE3 pKa = 4.0 SLGRR7 pKa = 11.84 SFDD10 pKa = 3.58 VLQQVMLVHH19 pKa = 5.99 YY20 pKa = 9.52 EE21 pKa = 3.98 RR22 pKa = 11.84 GSKK25 pKa = 10.15 RR26 pKa = 11.84 LSDD29 pKa = 4.62 QIIFWEE35 pKa = 4.16 AVRR38 pKa = 11.84 RR39 pKa = 11.84 EE40 pKa = 3.98 NALLHH45 pKa = 5.37 YY46 pKa = 10.26 ARR48 pKa = 11.84 KK49 pKa = 9.36 QRR51 pKa = 11.84 IARR54 pKa = 11.84 LGYY57 pKa = 8.53 TPVPSSATSEE67 pKa = 3.97 AAAKK71 pKa = 10.16 NAIKK75 pKa = 8.25 MTLLLTSLNEE85 pKa = 3.98 SEE87 pKa = 5.2 YY88 pKa = 11.04 KK89 pKa = 10.62 DD90 pKa = 4.47 EE91 pKa = 4.57 PWTMSDD97 pKa = 3.17 TSLEE101 pKa = 3.97 MLQCKK106 pKa = 9.84 PKK108 pKa = 10.74 GCFKK112 pKa = 10.83 KK113 pKa = 10.48 RR114 pKa = 11.84 GFTVHH119 pKa = 6.23 VLFDD123 pKa = 4.31 GDD125 pKa = 4.04 PGNSFPYY132 pKa = 9.46 TSWGEE137 pKa = 3.67 IYY139 pKa = 10.55 YY140 pKa = 10.79 QNLNDD145 pKa = 3.63 KK146 pKa = 9.25 WIKK149 pKa = 9.84 TEE151 pKa = 4.06 GKK153 pKa = 9.59 VDD155 pKa = 3.76 YY156 pKa = 10.92 DD157 pKa = 3.27 GLYY160 pKa = 8.86 YY161 pKa = 10.35 TDD163 pKa = 4.23 EE164 pKa = 5.85 DD165 pKa = 4.5 GEE167 pKa = 4.18 KK168 pKa = 10.44 LYY170 pKa = 11.51 YY171 pKa = 9.31 EE172 pKa = 4.7 TFAKK176 pKa = 10.62 DD177 pKa = 3.11 AMRR180 pKa = 11.84 FGTTGMWKK188 pKa = 9.11 VCYY191 pKa = 9.97 KK192 pKa = 9.97 STVLSALVSSSGTPAPSSVLWSQEE216 pKa = 3.17 WARR219 pKa = 11.84 EE220 pKa = 4.06 GGLEE224 pKa = 4.14 TQNPPVPATSDD235 pKa = 3.05 SRR237 pKa = 11.84 QPEE240 pKa = 4.27 SSVSHH245 pKa = 6.8 ASGTSTGSFARR256 pKa = 11.84 PGGEE260 pKa = 3.98 ACSSTPYY267 pKa = 10.27 RR268 pKa = 11.84 RR269 pKa = 11.84 GQEE272 pKa = 3.84 GGRR275 pKa = 11.84 GRR277 pKa = 11.84 GRR279 pKa = 11.84 GRR281 pKa = 11.84 GRR283 pKa = 11.84 GRR285 pKa = 11.84 GRR287 pKa = 11.84 GRR289 pKa = 11.84 PAGRR293 pKa = 11.84 QEE295 pKa = 4.18 GTPGSPSRR303 pKa = 11.84 STLPCSVLGRR313 pKa = 11.84 QGAQKK318 pKa = 9.94 RR319 pKa = 11.84 QRR321 pKa = 11.84 EE322 pKa = 4.04 ASPNRR327 pKa = 11.84 GSEE330 pKa = 3.74 QDD332 pKa = 3.06 RR333 pKa = 11.84 GGGEE337 pKa = 4.19 GGQGEE342 pKa = 4.73 SGEE345 pKa = 4.44 SASNEE350 pKa = 4.0 PEE352 pKa = 4.17 STNSPRR358 pKa = 11.84 LPRR361 pKa = 11.84 LSTPGSVSRR370 pKa = 11.84 VGGPCRR376 pKa = 11.84 PSPRR380 pKa = 11.84 KK381 pKa = 9.31 RR382 pKa = 11.84 APPRR386 pKa = 11.84 LSPAVSYY393 pKa = 10.38 RR394 pKa = 11.84 QVGGGCGGSEE404 pKa = 4.2 GQGKK408 pKa = 9.16 LLGGCTEE415 pKa = 4.49 GEE417 pKa = 4.17 DD418 pKa = 3.88 RR419 pKa = 11.84 PTPILIFRR427 pKa = 11.84 GDD429 pKa = 3.76 GNTQKK434 pKa = 10.32 CWRR437 pKa = 11.84 RR438 pKa = 11.84 RR439 pKa = 11.84 LRR441 pKa = 11.84 IRR443 pKa = 11.84 HH444 pKa = 5.33 SSRR447 pKa = 11.84 YY448 pKa = 7.85 MCASTGFSWTIPRR461 pKa = 11.84 GPNTVGRR468 pKa = 11.84 HH469 pKa = 4.45 RR470 pKa = 11.84 MLLAFNSDD478 pKa = 3.26 SQRR481 pKa = 11.84 AAFLEE486 pKa = 4.6 TVTIPRR492 pKa = 11.84 GLEE495 pKa = 3.69 FSEE498 pKa = 5.88 GYY500 pKa = 10.28 LDD502 pKa = 5.12 SII504 pKa = 5.19
Molecular weight: 55.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.928
IPC2_protein 8.916
IPC_protein 8.946
Toseland 9.399
ProMoST 9.311
Dawson 9.736
Bjellqvist 9.56
Wikipedia 9.94
Rodwell 9.882
Grimsley 9.823
Solomon 9.78
Lehninger 9.721
Nozaki 9.648
DTASelect 9.487
Thurlkill 9.575
EMBOSS 9.867
Sillero 9.721
Patrickios 4.952
IPC_peptide 9.765
IPC2_peptide 8.521
IPC2.peptide.svr19 7.818
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2603
98
605
371.9
41.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.34 ± 0.324
2.536 ± 0.737
5.839 ± 0.708
6.531 ± 0.632
4.034 ± 0.684
8.529 ± 1.164
2.113 ± 0.356
3.765 ± 0.732
4.495 ± 0.701
8.759 ± 1.019
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.614 ± 0.396
3.803 ± 0.744
6.954 ± 1.039
4.495 ± 0.561
6.723 ± 1.055
8.951 ± 0.903
5.839 ± 0.677
5.532 ± 0.717
1.268 ± 0.329
2.881 ± 0.415
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here