Thermomonospora echinospora

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Thermomonospora

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7946 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H6D9Z9|A0A1H6D9Z9_9ACTN Transcriptional regulator contains XRE-family HTH domain OS=Thermomonospora echinospora OX=1992 GN=SAMN04489712_1157 PE=4 SV=1
MM1 pKa = 7.93PEE3 pKa = 3.73MTSYY7 pKa = 11.19EE8 pKa = 4.0PGEE11 pKa = 4.24PCWADD16 pKa = 3.46LASPDD21 pKa = 3.73PEE23 pKa = 4.13AAKK26 pKa = 10.6VFYY29 pKa = 10.69GGLFGWGSSTPADD42 pKa = 4.04PGFGGYY48 pKa = 8.07TSFTLGGVEE57 pKa = 3.91GDD59 pKa = 3.61EE60 pKa = 4.37VAGLMEE66 pKa = 5.82LMDD69 pKa = 4.34EE70 pKa = 4.34RR71 pKa = 11.84QPPAWTCYY79 pKa = 10.33ISVADD84 pKa = 4.26ADD86 pKa = 4.03EE87 pKa = 4.32TADD90 pKa = 3.54AVTAAGGQVYY100 pKa = 7.78TPPMDD105 pKa = 3.37VAGLGRR111 pKa = 11.84MAVFGDD117 pKa = 3.5SQGAVFGVWEE127 pKa = 4.7PGDD130 pKa = 3.33FAGARR135 pKa = 11.84RR136 pKa = 11.84VNEE139 pKa = 4.13PGALCWTEE147 pKa = 3.65LACRR151 pKa = 11.84DD152 pKa = 3.37IEE154 pKa = 4.2AAKK157 pKa = 10.49AFYY160 pKa = 10.53GSVIGWEE167 pKa = 4.53SVTEE171 pKa = 4.07PAGTTTYY178 pKa = 10.66TEE180 pKa = 3.93WTTDD184 pKa = 2.92GRR186 pKa = 11.84SIAGMVQMNEE196 pKa = 4.02EE197 pKa = 4.53WPDD200 pKa = 3.53DD201 pKa = 4.02APPYY205 pKa = 8.16WMPYY209 pKa = 9.44FAVEE213 pKa = 4.39DD214 pKa = 4.01CDD216 pKa = 3.83AAADD220 pKa = 3.86KK221 pKa = 10.94AVALGGRR228 pKa = 11.84VPVPPTDD235 pKa = 3.36IPPGRR240 pKa = 11.84FAVVADD246 pKa = 3.88PDD248 pKa = 3.89GAYY251 pKa = 10.78LSIIRR256 pKa = 11.84LTGG259 pKa = 3.14

Molecular weight: 27.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H5VGX3|A0A1H5VGX3_9ACTN Helix-turn-helix OS=Thermomonospora echinospora OX=1992 GN=SAMN04489712_102322 PE=4 SV=1
MM1 pKa = 6.8SQKK4 pKa = 10.08SRR6 pKa = 11.84KK7 pKa = 7.98NRR9 pKa = 11.84RR10 pKa = 11.84TSARR14 pKa = 11.84TAPGRR19 pKa = 11.84RR20 pKa = 11.84SLTVRR25 pKa = 11.84IASGALGALAGGLAGRR41 pKa = 11.84ALRR44 pKa = 11.84PAARR48 pKa = 11.84QISRR52 pKa = 11.84RR53 pKa = 11.84LGVSAEE59 pKa = 3.85SMIRR63 pKa = 11.84VVEE66 pKa = 3.9ITAPVVASMAAGQLAARR83 pKa = 11.84GAARR87 pKa = 11.84NRR89 pKa = 11.84PHH91 pKa = 7.67RR92 pKa = 11.84ARR94 pKa = 11.84ILRR97 pKa = 11.84TRR99 pKa = 11.84TARR102 pKa = 11.84VRR104 pKa = 11.84GSGRR108 pKa = 11.84SRR110 pKa = 11.84LIPQHH115 pKa = 6.3PPSTASKK122 pKa = 10.34NN123 pKa = 3.52

Molecular weight: 13.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7946

0

7946

2513009

25

4309

316.3

34.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.414 ± 0.038

0.819 ± 0.008

5.901 ± 0.021

5.812 ± 0.022

2.694 ± 0.015

9.566 ± 0.03

2.267 ± 0.014

3.393 ± 0.018

1.84 ± 0.021

10.381 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.864 ± 0.012

1.674 ± 0.015

6.387 ± 0.03

2.71 ± 0.016

8.868 ± 0.031

4.581 ± 0.017

5.721 ± 0.023

8.522 ± 0.029

1.536 ± 0.013

2.05 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski