Erysiphe necator mitovirus 1
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I5ARE7|A0A2I5ARE7_9VIRU RNA-dependent RNA polymerase OS=Erysiphe necator mitovirus 1 OX=2052561 PE=4 SV=1
MM1 pKa = 7.74 AGEE4 pKa = 4.71 SCCSVQKK11 pKa = 11.02 DD12 pKa = 3.41 HH13 pKa = 7.36 VLYY16 pKa = 10.18 LWGWIEE22 pKa = 3.93 TSLKK26 pKa = 10.71 HH27 pKa = 6.38 KK28 pKa = 10.32 GLSHH32 pKa = 7.34 TILSLKK38 pKa = 8.14 TMRR41 pKa = 11.84 NCVTKK46 pKa = 10.27 YY47 pKa = 10.13 IAGEE51 pKa = 3.79 PVKK54 pKa = 10.73 VVEE57 pKa = 4.86 IRR59 pKa = 11.84 LSLTKK64 pKa = 10.61 DD65 pKa = 4.32 GIPKK69 pKa = 10.42 ALGPLVPIIRR79 pKa = 11.84 KK80 pKa = 9.46 LEE82 pKa = 4.2 PQDD85 pKa = 3.54 LRR87 pKa = 11.84 IVLTLLSVGKK97 pKa = 9.96 LYY99 pKa = 10.51 PGDD102 pKa = 3.45 GTVNTKK108 pKa = 10.66 SITDD112 pKa = 3.8 PFSGSMSKK120 pKa = 10.04 LHH122 pKa = 6.76 EE123 pKa = 4.09 YY124 pKa = 10.66 ALRR127 pKa = 11.84 GSFILEE133 pKa = 3.69 NLFKK137 pKa = 11.12 NQLTQLDD144 pKa = 4.41 IEE146 pKa = 4.9 QSSNWNAPHH155 pKa = 7.46 LSTKK159 pKa = 10.06 KK160 pKa = 10.8 GPIGHH165 pKa = 7.4 ALGTSMIEE173 pKa = 4.6 LGHH176 pKa = 7.45 LPDD179 pKa = 4.63 KK180 pKa = 11.1 LLVQIIKK187 pKa = 10.75 LGGLKK192 pKa = 10.06 LRR194 pKa = 11.84 NYY196 pKa = 9.08 ILSIIKK202 pKa = 8.66 MARR205 pKa = 11.84 TISKK209 pKa = 10.28 FEE211 pKa = 4.06 NSKK214 pKa = 10.88 KK215 pKa = 10.18 PIPKK219 pKa = 9.64 VYY221 pKa = 10.19 RR222 pKa = 11.84 KK223 pKa = 10.24 LSIVKK228 pKa = 10.33 DD229 pKa = 3.63 KK230 pKa = 10.65 EE231 pKa = 4.26 LKK233 pKa = 9.98 NRR235 pKa = 11.84 PIAIFDD241 pKa = 3.62 YY242 pKa = 10.11 WSQSALKK249 pKa = 8.77 PLHH252 pKa = 6.78 DD253 pKa = 3.91 SVMNLLRR260 pKa = 11.84 TFKK263 pKa = 10.62 TDD265 pKa = 3.08 LTFNQEE271 pKa = 3.73 GCEE274 pKa = 4.22 EE275 pKa = 4.24 IPSLIGPQAKK285 pKa = 9.98 LFSLDD290 pKa = 3.65 LSSATDD296 pKa = 3.69 RR297 pKa = 11.84 FPVEE301 pKa = 3.75 FQEE304 pKa = 4.79 IIVSRR309 pKa = 11.84 LIGQEE314 pKa = 3.62 RR315 pKa = 11.84 AEE317 pKa = 3.84 AWRR320 pKa = 11.84 YY321 pKa = 10.33 IMTEE325 pKa = 4.06 FPFNTPEE332 pKa = 4.49 GDD334 pKa = 3.61 SVKK337 pKa = 10.75 YY338 pKa = 8.79 NTGQPMGGYY347 pKa = 9.81 SSWCIFTLCHH357 pKa = 6.18 HH358 pKa = 6.86 LVVQIAYY365 pKa = 10.26 NEE367 pKa = 4.15 AYY369 pKa = 9.23 NRR371 pKa = 11.84 NWSRR375 pKa = 11.84 GARR378 pKa = 11.84 SGLPITPKK386 pKa = 10.42 LFEE389 pKa = 5.29 DD390 pKa = 3.97 YY391 pKa = 10.53 RR392 pKa = 11.84 ILGDD396 pKa = 6.0 DD397 pKa = 3.21 IVIWDD402 pKa = 3.62 EE403 pKa = 3.96 MVARR407 pKa = 11.84 SYY409 pKa = 11.31 KK410 pKa = 10.7 SIMADD415 pKa = 3.15 LGVEE419 pKa = 4.0 ISPFKK424 pKa = 10.31 SHH426 pKa = 6.21 EE427 pKa = 4.36 SYY429 pKa = 8.76 HH430 pKa = 4.93 TFEE433 pKa = 3.81 MAKK436 pKa = 9.65 RR437 pKa = 11.84 WFHH440 pKa = 6.73 KK441 pKa = 9.62 GTEE444 pKa = 4.02 ITGFPLNNLLEE455 pKa = 4.68 AKK457 pKa = 10.08 SNPWGIGKK465 pKa = 9.7 ALSDD469 pKa = 4.57 GIWRR473 pKa = 11.84 GWITKK478 pKa = 9.91 SNQGVPGNILIRR490 pKa = 11.84 RR491 pKa = 11.84 LLYY494 pKa = 10.58 SLGWSKK500 pKa = 11.0 RR501 pKa = 11.84 SINNRR506 pKa = 11.84 EE507 pKa = 3.62 EE508 pKa = 4.1 SVRR511 pKa = 11.84 DD512 pKa = 3.29 SFLFFNFSVTRR523 pKa = 11.84 KK524 pKa = 7.52 TDD526 pKa = 3.03 WVGEE530 pKa = 3.92 RR531 pKa = 11.84 SVRR534 pKa = 11.84 EE535 pKa = 3.82 FVSRR539 pKa = 11.84 HH540 pKa = 4.99 GPGQTMYY547 pKa = 7.93 TTQSARR553 pKa = 11.84 QEE555 pKa = 3.85 LAEE558 pKa = 4.07 VVRR561 pKa = 11.84 RR562 pKa = 11.84 IRR564 pKa = 11.84 VKK566 pKa = 11.01 GLLSLLNKK574 pKa = 9.72 GQEE577 pKa = 4.37 LIEE580 pKa = 4.2 KK581 pKa = 9.97 RR582 pKa = 11.84 NTAFGDD588 pKa = 3.62 YY589 pKa = 10.39 LRR591 pKa = 11.84 SLKK594 pKa = 10.82 DD595 pKa = 3.32 CGLPPTAFSLIEE607 pKa = 4.54 SIPVFQVLDD616 pKa = 3.98 RR617 pKa = 11.84 NQDD620 pKa = 3.91 LIKK623 pKa = 10.6 QAVIDD628 pKa = 3.97 LEE630 pKa = 4.54 GTNEE634 pKa = 3.91 EE635 pKa = 4.49 YY636 pKa = 10.96 ALSLDD641 pKa = 3.6 EE642 pKa = 4.68 SLSIGADD649 pKa = 3.2 LVVPDD654 pKa = 5.03 PDD656 pKa = 4.15 YY657 pKa = 11.65 IMSEE661 pKa = 3.92 RR662 pKa = 11.84 KK663 pKa = 9.1 SRR665 pKa = 11.84 LIIKK669 pKa = 10.05 QNAKK673 pKa = 8.54 TLATLLCAFEE683 pKa = 5.35 DD684 pKa = 3.98 PLIIIDD690 pKa = 6.08 DD691 pKa = 4.7 DD692 pKa = 4.46 DD693 pKa = 4.62 QDD695 pKa = 3.81 TCIEE699 pKa = 4.48 DD700 pKa = 5.15 DD701 pKa = 4.28 EE702 pKa = 6.42 DD703 pKa = 4.56 SEE705 pKa = 4.93 PDD707 pKa = 3.44 SLFQGLYY714 pKa = 10.49 DD715 pKa = 5.45 EE716 pKa = 5.11 EE717 pKa = 5.47 PDD719 pKa = 3.29 WSAMM723 pKa = 3.64
Molecular weight: 81.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.197
IPC2_protein 6.186
IPC_protein 6.262
Toseland 6.517
ProMoST 6.62
Dawson 6.59
Bjellqvist 6.576
Wikipedia 6.605
Rodwell 6.576
Grimsley 6.649
Solomon 6.59
Lehninger 6.59
Nozaki 6.854
DTASelect 7.015
Thurlkill 7.044
EMBOSS 7.015
Sillero 6.971
Patrickios 4.457
IPC_peptide 6.605
IPC2_peptide 6.751
IPC2.peptide.svr19 6.789
Protein with the highest isoelectric point:
>tr|A0A2I5ARE7|A0A2I5ARE7_9VIRU RNA-dependent RNA polymerase OS=Erysiphe necator mitovirus 1 OX=2052561 PE=4 SV=1
MM1 pKa = 7.74 AGEE4 pKa = 4.71 SCCSVQKK11 pKa = 11.02 DD12 pKa = 3.41 HH13 pKa = 7.36 VLYY16 pKa = 10.18 LWGWIEE22 pKa = 3.93 TSLKK26 pKa = 10.71 HH27 pKa = 6.38 KK28 pKa = 10.32 GLSHH32 pKa = 7.34 TILSLKK38 pKa = 8.14 TMRR41 pKa = 11.84 NCVTKK46 pKa = 10.27 YY47 pKa = 10.13 IAGEE51 pKa = 3.79 PVKK54 pKa = 10.73 VVEE57 pKa = 4.86 IRR59 pKa = 11.84 LSLTKK64 pKa = 10.61 DD65 pKa = 4.32 GIPKK69 pKa = 10.42 ALGPLVPIIRR79 pKa = 11.84 KK80 pKa = 9.46 LEE82 pKa = 4.2 PQDD85 pKa = 3.54 LRR87 pKa = 11.84 IVLTLLSVGKK97 pKa = 9.96 LYY99 pKa = 10.51 PGDD102 pKa = 3.45 GTVNTKK108 pKa = 10.66 SITDD112 pKa = 3.8 PFSGSMSKK120 pKa = 10.04 LHH122 pKa = 6.76 EE123 pKa = 4.09 YY124 pKa = 10.66 ALRR127 pKa = 11.84 GSFILEE133 pKa = 3.69 NLFKK137 pKa = 11.12 NQLTQLDD144 pKa = 4.41 IEE146 pKa = 4.9 QSSNWNAPHH155 pKa = 7.46 LSTKK159 pKa = 10.06 KK160 pKa = 10.8 GPIGHH165 pKa = 7.4 ALGTSMIEE173 pKa = 4.6 LGHH176 pKa = 7.45 LPDD179 pKa = 4.63 KK180 pKa = 11.1 LLVQIIKK187 pKa = 10.75 LGGLKK192 pKa = 10.06 LRR194 pKa = 11.84 NYY196 pKa = 9.08 ILSIIKK202 pKa = 8.66 MARR205 pKa = 11.84 TISKK209 pKa = 10.28 FEE211 pKa = 4.06 NSKK214 pKa = 10.88 KK215 pKa = 10.18 PIPKK219 pKa = 9.64 VYY221 pKa = 10.19 RR222 pKa = 11.84 KK223 pKa = 10.24 LSIVKK228 pKa = 10.33 DD229 pKa = 3.63 KK230 pKa = 10.65 EE231 pKa = 4.26 LKK233 pKa = 9.98 NRR235 pKa = 11.84 PIAIFDD241 pKa = 3.62 YY242 pKa = 10.11 WSQSALKK249 pKa = 8.77 PLHH252 pKa = 6.78 DD253 pKa = 3.91 SVMNLLRR260 pKa = 11.84 TFKK263 pKa = 10.62 TDD265 pKa = 3.08 LTFNQEE271 pKa = 3.73 GCEE274 pKa = 4.22 EE275 pKa = 4.24 IPSLIGPQAKK285 pKa = 9.98 LFSLDD290 pKa = 3.65 LSSATDD296 pKa = 3.69 RR297 pKa = 11.84 FPVEE301 pKa = 3.75 FQEE304 pKa = 4.79 IIVSRR309 pKa = 11.84 LIGQEE314 pKa = 3.62 RR315 pKa = 11.84 AEE317 pKa = 3.84 AWRR320 pKa = 11.84 YY321 pKa = 10.33 IMTEE325 pKa = 4.06 FPFNTPEE332 pKa = 4.49 GDD334 pKa = 3.61 SVKK337 pKa = 10.75 YY338 pKa = 8.79 NTGQPMGGYY347 pKa = 9.81 SSWCIFTLCHH357 pKa = 6.18 HH358 pKa = 6.86 LVVQIAYY365 pKa = 10.26 NEE367 pKa = 4.15 AYY369 pKa = 9.23 NRR371 pKa = 11.84 NWSRR375 pKa = 11.84 GARR378 pKa = 11.84 SGLPITPKK386 pKa = 10.42 LFEE389 pKa = 5.29 DD390 pKa = 3.97 YY391 pKa = 10.53 RR392 pKa = 11.84 ILGDD396 pKa = 6.0 DD397 pKa = 3.21 IVIWDD402 pKa = 3.62 EE403 pKa = 3.96 MVARR407 pKa = 11.84 SYY409 pKa = 11.31 KK410 pKa = 10.7 SIMADD415 pKa = 3.15 LGVEE419 pKa = 4.0 ISPFKK424 pKa = 10.31 SHH426 pKa = 6.21 EE427 pKa = 4.36 SYY429 pKa = 8.76 HH430 pKa = 4.93 TFEE433 pKa = 3.81 MAKK436 pKa = 9.65 RR437 pKa = 11.84 WFHH440 pKa = 6.73 KK441 pKa = 9.62 GTEE444 pKa = 4.02 ITGFPLNNLLEE455 pKa = 4.68 AKK457 pKa = 10.08 SNPWGIGKK465 pKa = 9.7 ALSDD469 pKa = 4.57 GIWRR473 pKa = 11.84 GWITKK478 pKa = 9.91 SNQGVPGNILIRR490 pKa = 11.84 RR491 pKa = 11.84 LLYY494 pKa = 10.58 SLGWSKK500 pKa = 11.0 RR501 pKa = 11.84 SINNRR506 pKa = 11.84 EE507 pKa = 3.62 EE508 pKa = 4.1 SVRR511 pKa = 11.84 DD512 pKa = 3.29 SFLFFNFSVTRR523 pKa = 11.84 KK524 pKa = 7.52 TDD526 pKa = 3.03 WVGEE530 pKa = 3.92 RR531 pKa = 11.84 SVRR534 pKa = 11.84 EE535 pKa = 3.82 FVSRR539 pKa = 11.84 HH540 pKa = 4.99 GPGQTMYY547 pKa = 7.93 TTQSARR553 pKa = 11.84 QEE555 pKa = 3.85 LAEE558 pKa = 4.07 VVRR561 pKa = 11.84 RR562 pKa = 11.84 IRR564 pKa = 11.84 VKK566 pKa = 11.01 GLLSLLNKK574 pKa = 9.72 GQEE577 pKa = 4.37 LIEE580 pKa = 4.2 KK581 pKa = 9.97 RR582 pKa = 11.84 NTAFGDD588 pKa = 3.62 YY589 pKa = 10.39 LRR591 pKa = 11.84 SLKK594 pKa = 10.82 DD595 pKa = 3.32 CGLPPTAFSLIEE607 pKa = 4.54 SIPVFQVLDD616 pKa = 3.98 RR617 pKa = 11.84 NQDD620 pKa = 3.91 LIKK623 pKa = 10.6 QAVIDD628 pKa = 3.97 LEE630 pKa = 4.54 GTNEE634 pKa = 3.91 EE635 pKa = 4.49 YY636 pKa = 10.96 ALSLDD641 pKa = 3.6 EE642 pKa = 4.68 SLSIGADD649 pKa = 3.2 LVVPDD654 pKa = 5.03 PDD656 pKa = 4.15 YY657 pKa = 11.65 IMSEE661 pKa = 3.92 RR662 pKa = 11.84 KK663 pKa = 9.1 SRR665 pKa = 11.84 LIIKK669 pKa = 10.05 QNAKK673 pKa = 8.54 TLATLLCAFEE683 pKa = 5.35 DD684 pKa = 3.98 PLIIIDD690 pKa = 6.08 DD691 pKa = 4.7 DD692 pKa = 4.46 DD693 pKa = 4.62 QDD695 pKa = 3.81 TCIEE699 pKa = 4.48 DD700 pKa = 5.15 DD701 pKa = 4.28 EE702 pKa = 6.42 DD703 pKa = 4.56 SEE705 pKa = 4.93 PDD707 pKa = 3.44 SLFQGLYY714 pKa = 10.49 DD715 pKa = 5.45 EE716 pKa = 5.11 EE717 pKa = 5.47 PDD719 pKa = 3.29 WSAMM723 pKa = 3.64
Molecular weight: 81.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.197
IPC2_protein 6.186
IPC_protein 6.262
Toseland 6.517
ProMoST 6.62
Dawson 6.59
Bjellqvist 6.576
Wikipedia 6.605
Rodwell 6.576
Grimsley 6.649
Solomon 6.59
Lehninger 6.59
Nozaki 6.854
DTASelect 7.015
Thurlkill 7.044
EMBOSS 7.015
Sillero 6.971
Patrickios 4.457
IPC_peptide 6.605
IPC2_peptide 6.751
IPC2.peptide.svr19 6.789
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
723
723
723
723.0
81.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.426 ± 0.0
1.245 ± 0.0
5.947 ± 0.0
6.639 ± 0.0
3.873 ± 0.0
6.501 ± 0.0
1.936 ± 0.0
8.022 ± 0.0
6.777 ± 0.0
11.48 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.936 ± 0.0
4.011 ± 0.0
4.841 ± 0.0
3.32 ± 0.0
5.394 ± 0.0
8.575 ± 0.0
5.118 ± 0.0
4.979 ± 0.0
2.075 ± 0.0
2.905 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here