Microbotryum saponariae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Pucciniomycotina; Microbotryomycetes; Microbotryales; Microbotryaceae; Microbotryum

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10396 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2X0NA48|A0A2X0NA48_9BASI BZ3500_MvSof-1268-A1-R1_Chr7-3g09567 protein OS=Microbotryum saponariae OX=289078 GN=BZ3500_MVSOF-1268-A1-R1_CHR7-3G09567 PE=4 SV=1
MM1 pKa = 8.06DD2 pKa = 4.53EE3 pKa = 5.44DD4 pKa = 3.93EE5 pKa = 4.52WSSASGDD12 pKa = 3.59VEE14 pKa = 4.74DD15 pKa = 4.44PQFRR19 pKa = 11.84PHH21 pKa = 5.48QVVRR25 pKa = 11.84IQLFDD30 pKa = 3.21QLYY33 pKa = 8.14YY34 pKa = 11.04VSDD37 pKa = 3.82VVIAKK42 pKa = 9.85SDD44 pKa = 3.61QCISTYY50 pKa = 10.92LFDD53 pKa = 3.43QLYY56 pKa = 8.5YY57 pKa = 10.94VSDD60 pKa = 3.82VVIAKK65 pKa = 10.12SDD67 pKa = 3.45EE68 pKa = 4.0

Molecular weight: 7.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2X0MU56|A0A2X0MU56_9BASI BZ3500_MvSof-1268-A1-R1_Chr3-3g06411 protein OS=Microbotryum saponariae OX=289078 GN=BZ3500_MVSOF-1268-A1-R1_CHR3-3G06411 PE=4 SV=1
MM1 pKa = 7.8ASLLRR6 pKa = 11.84NVFSLTSRR14 pKa = 11.84TRR16 pKa = 11.84FTSTRR21 pKa = 11.84PSPSSRR27 pKa = 11.84TTPTAWRR34 pKa = 11.84SPSASSISSTPSAASSWLLSRR55 pKa = 11.84PFSSTPLSQGLVRR68 pKa = 11.84KK69 pKa = 6.48PTKK72 pKa = 10.13IKK74 pKa = 10.95LKK76 pKa = 5.73THH78 pKa = 6.31KK79 pKa = 10.25GAAKK83 pKa = 9.84RR84 pKa = 11.84WFAIANGNFKK94 pKa = 10.52RR95 pKa = 11.84SQAGKK100 pKa = 10.3GVLSPTRR107 pKa = 11.84LNRR110 pKa = 11.84LGKK113 pKa = 9.18PAFARR118 pKa = 11.84PIEE121 pKa = 4.29KK122 pKa = 10.26KK123 pKa = 9.95KK124 pKa = 10.6LRR126 pKa = 11.84RR127 pKa = 11.84LMPYY131 pKa = 10.27AA132 pKa = 4.46

Molecular weight: 14.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10396

0

10396

5503069

34

5418

529.3

58.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.585 ± 0.023

1.216 ± 0.011

5.714 ± 0.018

5.7 ± 0.024

3.443 ± 0.012

6.758 ± 0.02

2.606 ± 0.011

4.203 ± 0.014

4.254 ± 0.021

9.222 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.805 ± 0.008

2.948 ± 0.011

6.494 ± 0.024

3.648 ± 0.015

6.98 ± 0.021

9.283 ± 0.034

6.411 ± 0.016

6.135 ± 0.015

1.322 ± 0.008

2.274 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski